Abstract
THE production of antibodies after exposure to antigens in vitro has been reported1–4 to occur with immune competent cells from nonimmunized animals, but antibody production in response to specific antigens does not seem to have been observed with cultured cells of established human lines. We have now exposed cells of the human lymphoblastoid line P3-J (“Jijoye”), taken from patients with Burkitt's lymphoma, to Coliphage T2 in vitro, and have found that the exposed cells appear to produce a factor capable of neutralizing the phage, probably an antibody. Roswell Park Memorial Institute (RPMI) cell lines5 No. 41 and No. 212, derived from human osteosarcoma and mesothelioma, respectively, produced no such factor.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Fishman, M., J. Exp. Med., 114, 837 (1961).
Saunders, G. C., and King, D. W., Science, 151, 1390 (1966).
Tao, T., and Uhr, J. W., Science, 151, 1096 (1966).
Mishell, R. I., and Dutton, R. W., Science, 153, 1004 (1966).
Moore, G. E., and Koike, A., Cancer, 17, 11 (1964).
Cleland, W. W., Biochemistry, 3, 480 (1964).
Tanigaki, N., Yagi, Y., Moore, G. E., and J. Immunol., 97, 634 (1966).
Fahey, J. L., Finegold, I., Robson, A. S., and Manaker, R. A., Science, 152, 1259 (1966).
Cohen, E. P., Newcomb, R. W., and Crosby, L. K., J. Immunol., 95, 583 (1965).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
KAMEI, H., MOORE, G. Production of Phage-neutralizing Activity in vitro by Cells derived from Burkitt's Lymphoma. Nature 215, 860–861 (1967). https://doi.org/10.1038/215860a0
Received:
Issue Date:
DOI: https://doi.org/10.1038/215860a0
This article is cited by
-
Identification of lymphoid cells by the use of immunofluorescent staining with antiserum against cultured lymphoblastoid cells
The Japanese Journal of Surgery (1972)
-
Human hemapoietic cell lines: A progress report
In Vitro (1969)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.