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Induction of lymphokine production by EAC and of blastogenesis by soluble mitogens during human B-cell activation

Nature volume 249, pages 834–837 (1974)Cite this article

Abstract

Two groups of human lymphocytes are distinguished on the basis of immune effector functions, membrane markers and in vitro mitogen reactivity. Thymus-derived (T) lymphocytes mediate cellular immunity either directly or by secreting pharmacologically active lymphokines1, and are implicated in facilitating antibody responses2. In contrast, bone marrow-derived (B) lymphocytes are thought to produce only antibodies. Studies in the guinea pig, however, indicate that, appropriately stimulated, B cells can produce macrophage migration inhibitory factor (MIF)3. We have now found that the erythrocyte–antibody (19S)–complement (EAC) rosetting method, used to obtain pure human peripheral blood B cells, activates these B cells to produce mononuclear cell chemotactic (CTX) and mitogenic (MF) lymphokines. Furthermore, we have investigated the mechanism of B cell activation by EAC and the effects of mitogenic lymphokines on human T and B lymphocytes.

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Author notes

  1. B. F. MACKLER

    Present address: University of Texas Dental Science Institute, PO Box 20068, Houston, Texas, 77025

Authors and Affiliations

  1. Laboratory of Microbiology and Immunology, National Institute of Dental Research, National Institutes of Health, Bethesda, Maryland, 20014

    B. F. MACKLER, L. C. ALTMAN, D. L. ROSENSTREICH & J. J. OPPENHEIM

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  1. B. F. MACKLER
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  2. L. C. ALTMAN
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  3. D. L. ROSENSTREICH
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  4. J. J. OPPENHEIM
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MACKLER, B., ALTMAN, L., ROSENSTREICH, D. et al. Induction of lymphokine production by EAC and of blastogenesis by soluble mitogens during human B-cell activation. Nature 249, 834–837 (1974). https://doi.org/10.1038/249834a0

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