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The application of high performance liquid chromatography, coupled to nuclear magnetic resonance spectroscopy and mass spectrometry (HPLC-NMR-MS), to the characterisation of ibuprofen metabolites from human urine

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Summary

The use of HPLC-NMR-MS for the detection and identification of the metabolites of ibuprofen present in a solid phase extract of human urine is described. Gradient reversed-phase HPLC was used to separate the components present in the extract, which were then characterised by a combination of stopped-flow1H NMR and on line electrospray-MS. This approach led to the rapid identification of the known phase 1 human metabolites of ibuprofen, including hydroxy- and carboxy- metabolites, together with their respective glucuronide conjugates. In addition a probable artefact resulting from the dehydration of one of the side chainhydroxylated glucuronides was also identified.

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  1. Zeneca Pharmaceuticals, Mereside, Alderley Park, SK10 4TG, Macclesfield, Cheshire, UK

    E. Clayton, S. Taylor, B. Wright & I. D. Wilson

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  1. E. Clayton
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  2. S. Taylor
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  3. B. Wright
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  4. I. D. Wilson
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Clayton, E., Taylor, S., Wright, B. et al. The application of high performance liquid chromatography, coupled to nuclear magnetic resonance spectroscopy and mass spectrometry (HPLC-NMR-MS), to the characterisation of ibuprofen metabolites from human urine. Chromatographia 47, 264–270 (1998). https://doi.org/10.1007/BF02466530

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  • DOI: https://doi.org/10.1007/BF02466530

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