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DNA is present in the pyrenoid core of the siphonous green algae of the genusCaulerpa and yellow-green algae of the genusPseudodichotomosiphon

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Summary

The pyrenoids of a number of siphonous green and yellow-green algae have been examined for the presence of chloroplast DNA (chloroplast nucleoids). We have found a conspicuous localization of chloroplast DNA in the pyrenoid core ofCaulerpa lentillifera andC. fergusonii (siphonous green algae), andPseudodichotomosiphon constrictus (a siphonous yellow-green alga) using fluorescence microscopy after staining with the DNA specific fluorochrome 4′,6-diamidino-2-phenylindole (DAPI).

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References

  • Calvert HE, Dawes CJ, Borowitzka MA (1976) Phylogenetic relationships ofCaulerpa (Chlorophyta) based on comparative chloroplast ultrastructure. J Phycol 12: 149–162

    Google Scholar 

  • Coleman AW (1985) Diversity of plastid DNA configuration among classes of eukaryote algae. J Phycol 21: 1–16

    Google Scholar 

  • Ehara T, Ogasawara Y, Osafune T, Hase E (1990) Behaviour of chloroplast nucleoids during the cell cycle ofChlamydomonas reinhardtii (Chlorophyta) in synchronized culture. J Phycol 26: 317–323

    Google Scholar 

  • Esser K (1967) Elektronenmikroskopischer Nachweis von DNS in den Pyrenoiden vonStroptotheca thamesis. Z Naturforsch 22 b: 993

    Google Scholar 

  • Goodenough UW (1970) Chloroplast division and pyrenoid formation inChlamydomonas reinhardtii. J Phycol 6: 1–6

    Google Scholar 

  • Hansmann P, Falk H, Scheer U, Sitte P (1986) Ultrastructural localization of DNA in twoCryptomonas species by use of a monoclonal DNA antibody. Eur J Cell Biol 42: 152–160

    Google Scholar 

  • Holdsworth RH (1971) The isolation and partial characterization of the pyrenoid protein ofEremosphaera viridis. J Cell Biol 51: 499–513

    Google Scholar 

  • Hori T (1974) Electron microscope observations on the fine structure of the chloroplasts of algae. II. The chloroplasts of Caulerpa (Chlorophyceae). Int Rev Ges Hydrobiol 59: 239–245

    Google Scholar 

  • —, Ueda R (1967) Electron microscope studies on the fine structure of plastids in siphonous green algae with special reference to their phylogenetic relationships. Sci Rep Tokyo Kyoiku Daigaku Sect B 12: 225–244

    Google Scholar 

  • —, Kobara T, Chihara M (1979) Electron microscope observation onPseudodichotomosiphon constrictus with special reference to the systematic position of the genus. Jap J Phycol 27: 183–191

    Google Scholar 

  • —, Inouye I (1981) The ultrastructure of mitosis inCricosphaera roscoffensis var.haptonemofera (Prymnesiophyceae). Protoplasma 106: 121–135

    Google Scholar 

  • Kirk JTO, Tilney-Bassett RAE (1978) The plastids: their chemistry, structure, growth, and inheritance. Elsevier/North-Holland, Amsterdam

    Google Scholar 

  • Kobara T, Chihara M (1990) On the taxonomic position of a siphonous algaPseudodichotomosiphon constrictus. J Jap Bot 65: 13–20

    Google Scholar 

  • Kuchitsu K, Tsuzuki M, Miyachi S (1988) Characterization of the pyrenoid isolated from unicellular green algaChlamydomonas reinhardtii: particulate form of RuBisCo protein. Protoplasma 144: 17–24

    Google Scholar 

  • Kuroiwa T, Suzuki T (1980) An improved method for the demonstration of the in situ chloroplast nuclei in higher plants. Cell Struct Funct 5: 195–197

    Google Scholar 

  • — —, Ogawa K, Kawano S (1981) Chloroplast nucleus — general occurrence, number, size, shape and a model for amplification of chloroplast genome during chloroplast development. Plant Cell Physiol 22: 381–396

    Google Scholar 

  • Lacoste-Royal G, Gibbs SP (1987) Immunocytochemical localization of ribulose-1,5-bisphosphate carboxylase in the pyrenoid and thylakoid region of the chloroplast ofChlamydomonas reinhardtii. Plant Physiol 83: 602–606

    Google Scholar 

  • Miyamura S, Hori T (1989) Presence of DNA in the pyrenoid matrix of the siphonous green alga,Caulerpa okamurae Web. v. Bos. Plant Morphol 1: 19–22

    Google Scholar 

  • Nakamura S, Itoh S, Kuroiwa T (1986) Behaviour of chloroplast nucleus during chloroplast development and degeneration inChlamydomonas reinhardtii. Plant Cell Physiol 27: 775–784

    Google Scholar 

  • Satoh H, Okada M, Nakayama K, Miyaji K (1984) Purification and further characterization of pyrenoid proteins and ribulose-1,5-bisphosphate carboxylase-oxygenase from the green algaBryopsis maxima. Plant Cell Physiol 25: 1205–1214

    Google Scholar 

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Authors and Affiliations

  1. Institute of Biological Sciences, University of Tsukuba, Tsukuba-shi, 305, Ibaraki, Japan

    S. Miyamura & T. Hori

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  1. S. Miyamura
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  2. T. Hori
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Miyamura, S., Hori, T. DNA is present in the pyrenoid core of the siphonous green algae of the genusCaulerpa and yellow-green algae of the genusPseudodichotomosiphon . Protoplasma 161, 192–196 (1991). https://doi.org/10.1007/BF01322731

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  • DOI: https://doi.org/10.1007/BF01322731

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