Summary
InEuglena gracilis Z, vitamin B12 deficiency arrests cell divisions in S/G 2 phase. After the addition of vitamin B12 to blocked cells, nuclear and cellular divisions begin to be induced between the 3rd and the 4th and between the 4th and the 5th hour respectively; the cell population is doubled after the 11th hour.
Addition of cycloheximide either with vitamin B12 or 2 to 6 hours later inhibits the resumption of divisions and blocks cell development in different stages between G 2, karyokinesis and cytokinesis. These results suggest that as a prerequisite protein-dependent steps are required at precise times during the reversibility of blocked cell divisions: at least sequential syntheses of protein concern a) formation of the mitotic spindle and replication of the pellicle; b) completion of the nuclear division; c) progression of the cleavage furrow.
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Bré, M.H., El Ferjani, E. & Lefort-Tran, M. Sequential protein-dependent steps in the cell cycle initiation and completion of division in vitamin B12 replenishedEuglena gracilis . Protoplasma 108, 301–318 (1981). https://doi.org/10.1007/BF02224425
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DOI: https://doi.org/10.1007/BF02224425