Abstract
The PV subfamily of Alu repeats in human DNA is largely composed of recently inserted members. Here we document additional members of the PV subfamily that are found in chimpanzee but not in the orthologous loci of human and gorilla, confirming the relatively recent and independent expansion of this Alu subfamily in the chimpanzee lineage. As further evidence for the youth of this Alu subfamily, one PV Alu repeat is specific to Pan troglodytes, whereas others are present in Pan paniscus as well. The A-rich tails of these Alu repeats have different lengths in Pan paniscus and Pan troglodytes. The dimorphisms caused by the presence and absence of PV Alu repeats and the length polymorphisms attributed to their A-rich tails should provide valuable genetic markers for molecular-based studies of chimpanzee relationships. The existence of lineage-specific Alu repeats is a major sequence difference between human and chimpanzee DNAs.
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References
Batzer MA, Deininger PL (1991) A human-specific subfamily of Alu sequences. Genomics 9:481–487
Batzer MA, Kilroy GE, Richard PE, Shaikh TH, Desselle TD, Hoppens CL, Deininger PL (1990) Structure and variability of recently inserted Alu family members. Nucleic Acids Res 18:6793–6798
Economou EP, Bergen AW, Warren AC, Antonarakis SE (1990) The polydeoxyadenylate tract of Alu repetitive elements is polymorphic in the human genome. Proc Natl Acad Sci USA 87:2951–2954
Friezner-Degen SJ, Rajput B, Reich E (1986) The tissue plasminogen-activator gene. J Biol Chem 261:6972–6985
Hellmann-Blumberg U, Hintz MF, Gatewood JM, Schmid CW (1993) Developmentally regulated demethylation of human Alu repeats. Mol Cell Biol (in press)
Jurka J, Smith T (1988) A fundamental division in the Alu family of repeated sequences. Proc Natl Acad Sci USA 85:4775–4778
Koop BF, Miyamoto MM, Embury JE, Goodman M, Czelusniak J, Slightom JL (1986) Nucleotide sequence and evolution of the orangutan epsilon globin gene region and surrounding Alu repeats. J Mol Evol 24:94–102
Leeflang EP, Liu W, Hashimoto C, Choudary PV, Schmid CW (1992) Phylogenetic evidence for multiple Alu source genes. J Mol Evol 35:7–16
Leeflang EP, Chesnokov IN, Liu W, Schmid CW (1993) Phylogenetic isolation of a human Alu founder gene: drift to new subfamily identity. J Mol Evol 37:559–565
Matera AG, Hellmann U, Hintz MF, Schmid CW (1990a) Recently transposed Alu repeats result from multiple source genes. Nucleic Acids Res 18:6019–6023
Matera AG, Hellmann U, Schmid CW (1990b) A transpositionally and transcriptionally competent Alu subfamily. Mol Cell Biol 10:5424–5432
Mietus-Snyder M, Charmley P, Korf B, Ladias JAA, Gatti RA, Karathanasis SK (1990) Genetic linkage of the human apolipoprotein AI-CIII-AIV gene cluster and the neural cell adhesion molecule (NCAM) gene. Genomics 7:633–637
Muratani K, Hada T, Yamamoto Y, Kaneko T, Shigeto Y, Ohue T, Furuyama J, Higashino K (1991) Inactivation of cholinesterase gene by Alu insertion: possible mechanism for human gene transposition. Proc Natl Acad Sci USA 88:11315–11319
Ryan SC, Dugaiczyk A (1989) Newly arisen DNA repeats in primate phylogeny. Proc Natl Acad Sci USA 86:9360–9364
Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning, Vol III, Appendix B: 1–28. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY
Sawada I, Schmid CW (1986) Primate evolution of the alpha-globin gene cluster and its Alu-like repeats. J Mol Biol 192: 693–709
Sawada I, Willard C, Shen C-KJ, Chapman B, Wilson AC, Schmid CW (1985) Evolution of Alu family repeats since the divergence of human and chimpanzee. J Mol Evol 22:316–322
Schmid CW (1991) Human Alu subfamilies and their methylation revealed by blot hybridization. Nuc Acids Res 20:5613–5617
Schmid CW, Shen C-KJ (1985) The evolution of interspersed repetitive DNA sequences in mammals and other vertebrates. In: MacIntyre RJ (ed) Molecular evolutionary genetics. Plenum, New York, pp 323–358
Stoppa-Lyonnet D, Carter PE, Meo T, Tose M (1990) Clusters of intragenic Alu repeats predispose the human C1 inhibitor locus to deleterious rearrangements. Proc Natl Acad Sci USA 87:1551–1555
Wallace MR, Andersen LB, Saulino AM, Gregory PE, Glover TW, Collins FS (1991) A de novo Alu insertion results in neurofibromatosis type 1. Nature 353:864–865
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Correspondence to: C.W. Schmid
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Leeflang, E.P., Chesnokov, I.N. & Schmid, C.W. Mobility of short interspersed repeats within the chimpanzee lineage. J Mol Evol 37, 566–572 (1993). https://doi.org/10.1007/BF00182742
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DOI: https://doi.org/10.1007/BF00182742