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Topographical studies on intestinal microvillous leucine β-naphthylamidase on the outer membrane surface

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Summary

The location of leucine β-naphthylamidase on the outer surface of the microvillous membrane of rabbit small intestine was examined by analyzing the interaction of antibodies against leucine β-naphthylamidase or another microvillous enzyme, sucrase-isomaltase complex, with microvillous vesicles having different relative amounts of these enzymes, in respect to vesicle agglutination, inhibition of enzyme activity, and electron-microscopic morphology. The results obtained indicate that leucine β-naphthylamidase, or at least its antigenic sites, protrude about 10 nm from the outer surface of the microvillous membrane.

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Authors and Affiliations

  1. Research Institute of Environmental Medicine, Nagoya University, Aichi Cancer Center Research Institute, 464, Nagoya, Aichi, Japan

    Yoshiki Takesue & Yoshimi Nishi

  2. Laboratory of Ultrastructure Research, Aichi Cancer Center Research Institute, 464, Nagoya, Aichi, Japan

    Yoshiki Takesue & Yoshimi Nishi

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  1. Yoshiki Takesue
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  2. Yoshimi Nishi
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Takesue, Y., Nishi, Y. Topographical studies on intestinal microvillous leucine β-naphthylamidase on the outer membrane surface. J. Membrain Biol. 39, 285–296 (1978). https://doi.org/10.1007/BF01869895

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  • DOI: https://doi.org/10.1007/BF01869895

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