Abstract
Intact mustard seedlings were treated with zeatin and photomorphogenetically active light in different ways: (1) hormone treatment preceding light treatment, (2) light treatment preceding hormone treatment, (3) hormone and light applied simultaneously. Under all experimental conditions the effect of the hormone treatment is multiplicative to the light effect with regard to the increase of cotyledon area. However, the hormone effect is additive to the light effect with regard to increases of the level of NADPH-dependent glyceraldehyde-3-phosphate dehydrogenase (EC 1.2.1.13) and carotenoid contents. Anthocyanin synthesis is inhibited by exogenous zeatin whereby the concentration response curves are similar, irrespective of the extent of anthocyanin formation mediated by light. However, an interaction was found in the sense that the responsiveness toward zeatin is decreased somewhat by the action of phytochrome. Our results show that the responsiveness to light (via the far-red-absorbing form of phytochrome; P fr) is not changed by a preceding or simultaneous hormone treatment. Moreover, the responsiveness of the plant to exogenously applied zeatin is not affected — except in anthocyanin synthesis — by a preceding or simultaneous light treatment. We conclude from our results that the action of phytochrome on the developmental processes is not related to cytokinin levels.
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Abbreviations
- GPD:
-
glyceraldehyde-3-phosphate dehydrogenase (NADP) (EC 1.2.1.13)
- P fr :
-
active (far-red-absorbing) form of phytochrome
- FR:
-
standardized far-red light
- RL:
-
standardized red light
References
Brüning, K., Drumm, H., Mohr, H. (1975) On the role of phytochrome in controlling enzyme levels in plastids. Biochem. Physiol. Pflanz. 168, 141–156
Bühler, B., Drumm, H., Mohr, H. (1978) Investigations on the role of ethylene in phytochrome-mediated photomorphogenesis. I. Anthocyanin synthesis. Planta 142, 109–117
Buschmann, C., Grumbach, K. (1981) The effect of phytochrome and white light of high and low intensity on the uptake and distribution of 14C-labelled kinetin in radish seedlings (Raphanus sativus). Physiol. Plant. 53, 518–522
DeGreef, J.A., Frédéricq, H. (1983) Photomorphogenesis and hormones. In: Encyclopedia of plant physiology, N.S., vol. 16: Photomorphogensis, pp. 401–427, Shorpshire, W., Mohr, H., eds. Springer, Berlin Heidelberg New York
Feierabend, J. (1969) Der Einfluß von Cytokininen auf die Bildung von Photosyntheseenzymen in Roggenkeimlingen. Planta 84, 11–28
Feierabend, J. (1981) Influence of cytokinin on plastid biogenesis in rye leaves. In: Metabolism and molecular activites of cytokinins. pp. 252–260, Guern, J., Péaud-Lenöel, C., eds. Springer, Berlin Heidelberg New York
Feierabend, J., de Boer, J. (1978) Comparative analysis of the action of cytokinin and light on the formation of ribulose-bisphosphate carboxylase and plastid biogenesis. Planta 142, 75–82
Ford, M.J., Kasemir, H., Mohr, H. (1981) The influence of phytochrome and kinetin on chlorophyll accumulation in mustard cotyledons: a two-factor analysis. Ber. Dtsch. Bot. Ges. 94, 35–41
Frosch, S., Mohr, H. (1980) Analysis of light-controlled accumulation of carotenoids in mustard (Sinapis alba L.) seedlings. Planta 148, 279–286
Iino, M. (1982) Inhibitory action of red light on the growth of the maize mesocotyl: evaluation of the auxin hypothesis. Planta 156, 388–395
Kaltofen, H. (1981) Koaktonsanalyse — eine Methodedes Zusammenwirkens zweier Einflußgrößen. Biol. Rundsch. 19, 155–173
Kandeler, R. (1974) Photomorphogenese. Die Rolle der Hormone. Ber. Dtsch. Bot. Ges. 87, 71–81
Kasemir, H., Mohr., H. (1982) Coaction of three factors controlling chlorophyll and anthocyanin synthesis Planta 156, 282–288
Keller, P.L., Coulter, M.W. (1982) The relationship of endogenous gibberellins to light-regulated stem elongation rates in dwarf and normal cultivars of Pisum sativum L. Plant Cell Physiol. 23, 409–416
Kochhar, V.K., Kochhar, S., Mohr, H. (1981) Action of light and kinetin on betalain synthesis in seedlings of Amaranthus caudatus: a two-factor analysis. Ber. Dtsch. Bot. Ges. 94, 27–34
Köhler, K.-H., Dörfler, M., Göhring, H. (1980) The influence of light on the cytokinin content of Amaranthus seedlings. Biol. Plant. 22, 128–134
Longo, C.P., Longo, G.P., Lampugnani, M., Rossi, G., Servettaz, O. (1981) Light and fusicoccin as tools for discriminating among responses of cotyledons to cytokins. In: Metabolism and molecular activities of cytokinins, pp. 261–265, Guern, J., Péaud-Lenöel, C., eds. Springer, Berlin Heidelberg New York
Moore, T.C. (1979) Biochemistry and physiology of plant hormones. Springer, New York Heidelberg Berlin
Ohya, T., Naito, K., Suzuki, H. (1981) Effect of benzyladenine on change in plastid membrane proteins of etiolated cucumber cotyledon during greening. Z. Pflanzenphysiol. 102, 167–172
Palmer, M.V., Horgan, R., Wareing, P.F. (1981) Cytokinin metabolism in Phaseolus vulgaris L. Planta 153, 297–302
Parthier, B., Lerbs, S., Klyachko, N.L. (1981) Plastogenesis and cytokinin action. Cytokinin and light interactions in plastid enzyme formation of detached Cucurbita cotyledons. In: Metabolism and molecular activities of cytokinins, pp. 275–286, Guern, J., Péaud-Lenöel, C., eds. Springer, Berlin Heidelberg New York
Pecket, R.C., Bassim, T.A.H. (1974) The effect of kinetin in relation to photocontrol of anthocyanin biosynthesis in Brassica oleracea. Phytochemistry 13, 1395–1399
Pfaff, W., Schopfer, P. (1980) Hormones are no causal links in phytochrome-mediated induction of phenylalanine ammonia-lyase in mustard (Sinapis alba, L.) cotyledons. Planta 150, 53–57
Schäfer, E. (1977) Kunstlicht und Pflanzenbau. In: Optische Strahlungsquellen, pp. 249–266, Albrecht, H., ed. Lexika, Grafenau
Schmidt, R., Mohr, H. (1982) Evidence that a mustard seedling responds to the amount of P fr and not to the P fr/P tot ratio. Plant Cell Environ. 5, 494–499
Shropshire, W., Mohr, H. (eds.) (1983) Photomorphogenesis. Encyclopedia of plant physiology, N.S., vol. 15: Photomorphogenesis. Springer, Berlin Heidelberg New York
Tong, Zhe, Lian Han-ping, Song Yan-ru, Tao Guo-quing, Chen Hui-ying, Tsui Cheng (1980) The relationship between the effects of cytokinin on the expansion and metabolism of excised cucumber cotyledons and water stress. Acta Bot. Sinica 22, 360–364
Walton, J.D., Ray, P.M. (1981) Evidence for receptor functior of auxin binding sites in maize Plant Physiol 68, 1334–1338
Wareing, P.F., Thompson, A.G. (1976) Rapid effects of red light on hormone levels. In: Light and plant development pp. 285–294, Smith, H., ed. Butterworths, London
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Tong, Z., Kasemir, H. & Mohr, H. Coaction of light and cytokinin in photomorphogenesis. Planta 159, 136–142 (1983). https://doi.org/10.1007/BF00392984
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DOI: https://doi.org/10.1007/BF00392984