Summary
The application of the semipermeable membrane technique in light microscopical demonstration of choline acetyltransferase is described. The method founds upon earlier developed lead salt techniques. Use of semipermeable membranes fully prevents any loss of enzyme by dissolvement or inactivation during fixation. Addition of NaCl to the incubation medium markedly increases the activity of choline acetyltransferase.
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The research reported in this paper was supported by the “Ministerium für Wissenschaft und Technik der DDR”
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Hüther, G., Luppa, H. The histochemical demonstration of choline acetyltransferase by semipermeable membrane technique. Histochemistry 49, 303–307 (1976). https://doi.org/10.1007/BF00496133
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DOI: https://doi.org/10.1007/BF00496133