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Determination of carbohydrates in fermentation processes by high-performance liquid chromatography

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Summary

HPLC is a universal, fast, accurate and selective method for the quantification of carbohydrates during fermentation processes. HPLC is not affected by complex constituents of fermentation media, such as meat extract, soybean meal or distillers solubles. The detection limit of the different investigated carbohydrates by refractive index monitoring ranges between 20 and 40 mg/l using a cation-exchange resin and between 50 and 100 mg/l using amino- or diol-bonded phases.

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Authors and Affiliations

  1. LB Mikrobiologie/Antibiotika, Universität Tübingen, Auf der Morgenstelle 28, D-7400, Tübingen, Federal Republic of Germany

    Armin Plaga, Joachim Stümpfel & Hans-Peter Fiedler

Authors
  1. Armin Plaga
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  2. Joachim Stümpfel
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  3. Hans-Peter Fiedler
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Offprint requests to: H.-P. Fiedler

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Plaga, A., Stümpfel, J. & Fiedler, HP. Determination of carbohydrates in fermentation processes by high-performance liquid chromatography. Appl Microbiol Biotechnol 32, 45–49 (1989). https://doi.org/10.1007/BF00164821

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  • DOI: https://doi.org/10.1007/BF00164821

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