Summary
Atrial natriuretic factor (ANF) binding sites have been shown to be present on human platelet membranes. We investigated the effect of an infusion of ANF 5 pmol·kg−1.min−1 on platelet aggregation in whole blood ex-vivo in 8 normal volunteers.
Spontaneous platelet aggregation, collagen (0.6–2 μg·ml−1)-induced or ADP (0.5–2.0 μM)-induced aggregation was not affected by ANF. Plasma aldosterone was however significantly attenuated by ANF.
These results show that a pharmacological dose of ANF does not affect platelet aggregation in man. These results suggest that the high plasma levels of ANF normally achieved in chronic heart failure or acute myocardial infarction are unlikely to contribute to the platelet hyperreactivity, often observed in these conditions.
References
Laragh JH (1985) Atrial natriuretic hormone, the renin-aldosterone-axis, and blood pressure-electrolyte homeostatis. N Engl J Med 313: 1330–1340
Leitman DC, Murad F (1987) Atrial natriuretic factor receptor hetereogeneity and stimulation of particulate guanylate cyclase and cyclic GMP accumulation. Endocrinol Metab Clin North Am 16: 79–105
Schiffrin EL, Deslongchamps M, Thibault G (1986) Platelet binding sites for atrial natriuretic factor in humans. Characterization and effects of sodium intake. Hypertension 8 [Suppl II]: II-6-II-10
Saniabadi AR, Lowe GD, Belch JJ, Forbes CD (1989) Platelet aggregation inhibitory effects of the new positive inotropic agents pimobendan and UD CG212 in whole blood. Cardiovas Res 23: 184–190
Saniabadi AR, Lowe G, Belch J, Barbenel JC, Forbes CD (1990) A platelet counting technique to study platelet aggregation in whole blood: application to pathologically low platelet counts. Platelets 1: 151–153
De Caterina R, Volpe M, Atlas SA, Weksler BB (1985) Effects of atrial natriuretic factor on human platelet function. Life Sci 37: 1395–1402
Loeb AL, Gear ARL (1988) Potentiation of platelet aggregation by atrial natriuretic peptide. Life Sci 43: 731–738
Schiffrin EL (1988) Decreased density of binding sites for atrial natriuretic peptide on platelets of patients with severe congestive heart failure. Clin Sci 74: 213–218
Hamet P, Tremblay J, Pang SC, Skuherska R, Schiffrin EL, Garcia R, Cantin M, Genest J, Palmour R, Ervin FR, Martin S, Goldwater R (1986) Cyclic GMP as mediator and biological marker of atrial natriuretic factor. J Hypertens 4 [Suppl 2]: S 49-S 56
Hamet P, Tremblay J, Pang SC, Garcia R, Thibault G, Gutkowska J, Cantin M, Genest J (1984) Effect of native and synthetic atrial natriuretic factor on cyclic GMP. Biochem Biophys Res Commun 123: 515–527
Raine AEG, Erne P, Burgisser E, Muller FB, Balli P, Burkart F, Buhler FR (1986) Atrial natriuretic peptide and atrial pressure in patients with congestive heart failure. N Engl J Med 315: 533–537
Tomoda H (1988) Atrial natriuretic peptide in acute myocardial infarction. Am J Card 62: 1122–1123
Jafri SM, Riddle JM, Raman SBK, Goldstein S (1986) Altered platelet function in patients with severe congestive heart failure. Henry Ford Hosp Med J 34: 156–159
Trip MD, Cats VM, Van Capelle FIL, Vreeken J (1990) Platelet hyperreactivity and prognosis in survivors of myocardial infarction. N Engl J Med 322: 1549–1554
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Lang, C.C., Lau, C.S., Belch, J.J. et al. Effect of atrial natriuretic factor on platelet function in whole blood ex-vivo in man. Eur J Clin Pharmacol 39, 589–591 (1990). https://doi.org/10.1007/BF00316102
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00316102