Abstract
A quantitative ultrastructural study was performed with samples taken throughout a layer of the purple sulfur bacterium Chromatium minus in Lake Cisó (Spain). Ultrathin sections of cells were analyzed by transmission electron microscopy, in order to study the size, number and volume of intracytoplasmic membranes (ICM), sulfur globules and poly-β-hydroxybutyrate (PHB) granules per unit volume of cell. Important differences were seen between cells from the top (receiving 60 μE · m−2 · s−1 at noon) on the one hand, and cells from the peak and bottom parts of the bacterial layer (receiving less than 1 μE · m−2 · s−1) on the other hand. The amount of ICM per cell increased as a function of depth being about three times higher in bottom cells than in top cells. Neither statistically significant differences in cell size, nor in numbers of sulfur globules were found, but the ultrastructure changed with depth. Finally, the most important changes throughout depth were detected in PHB granules. Top cells had 0.5% of their volume occupied by PHB granules, whereas in the bottom cells the corresponding value was 12.2%. These changes were due to the number of PHB granules per unit volume of cell since globule size was constant.
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Abbreviations
- ECM:
-
intracytoplasmic membrane systems
- PHB:
-
poly-β-hydroxybutyrate
- Bchl:
-
bacteriochlorophyll
- SED:
-
sphere equivalent diameter
References
Anderson TF (1951) Techniques for the preservation of three dimensional structure in preparing specimens for the electron microscope. Trans NY Acad Sci 13: 130–135
Breznak JA, Potrikus CJ, Pfennig N, Ensign JC (1978) Viability and endogenous substrates used during starvation survival of Rhodospirillum rubrum. J Bacteriol 134: 381–388
Broch-Due M, Ormerod JG, Strand F (1978) Effect of light intensity on vesicle formation in Chlorobium. Arch Microbiol 116: 269–274
Cohen-Bazire G, Pfennig N, Kunisawa R (1964) The fine structure of green bacteria. J Cell Biol 22: 207–225
Esteve I, Montesinos E, Abellá C, Guerrero R (1980) Changes in the ultrastructure of the purple sulfur bacteria Chromatium minus depending on the variation of light in a natural habitat. Electron Microscopy 2: 456–457
Fuller RC, Conti SF, Menlin DB (1963) The structure of the photosynthetic apparatus in the green and purple sulfur bacteria. In: San Pietro A, Vernon LP (eds) Bacterial photosynthesis. Antioch Press, Yellow Springs, Ohio, pp 71–87
Golecki JR, Schumacher A, Drews G (1980) The differentiation of the photosynthetic apparatus and the intracytoplasmic membrane in cells of Rhodopseudomonas capsulata upon variation of light intensity. Europ J Cell Biol 23: 1–5
Golterman HL, Clymo RS, Ohnstad AM (1978) Methods for physical and chemical analysis of fresh waters. IBP Handbook no. 8, Blackwell Scientific Publications, Oxford
Guerrero R, Montesinos E, Esteve I, Abellá C (1980) Physiological adaptation and growth of purple and green sulfur bacteria in a meromictic lake as compared to a holomictic lake. In: Dokulil M, Metz H, Jewson D (eds) Shallow lakes. Dr W Junk Publishers, The Hague, pp 161–171
Guerrero R, Montesinos E, Pedrós-Alió C, Esteve I, Mas J, Van Gemerden H, Hofman PAG, Bakker JF (1985) Phototrophic sulfur bacteria in two Spanish lakes: Vertical distribution and limiting factors. Limnol Oceanogr 30: 919–931
Holmqvist O (1979) Evidence of discontinuity between the cytoplasmic and intracytoplasmic membranes in Rhodopseudomonas sphaeroides. A study with ferrous gluconate as a tracer substance in electron microscopy and x-ray microanalysis. FEMS Microbiol Lett 6: 37–40
Holt SC, Marr AG (1965a) Location of chlorophyll in Rhodospirillum rubrum. J Bacteriol 89: 1402–1406
Holt SC, Marr AG (1965b) Isolation and purification of the intracytoplasmic membranes of Rhodospirillum rubrum. J Bacteriol 89: 1413–1418
Merrick JM (1978) Metabolism of reserve materials. In: Clayton RK, Sistrom WR (eds) The photosynthetic bacteria. Plenum Press, New York, pp 199–219
Montesinos E (1987) Change in size of Chromatium minus cells in relation to growth rate, sulfur content, and photosynthetic activity: a comparison of pure cultures and field populations. Appl Environ Microbiol 53: 864–871
Montesinos E, Esteve I (1984) Effect of algal shading on the net growth and production of phototrophic sulfur bacteria in lakes of the Banyoles karstic area. Verh Internat Verein Limnol 22: 1102–1105
Nie NH, Hull CH, Jenkins JG, Streinbrenner K, Bent DH (1975) Statistical package for the social sciences. 2nd edn. McGraw-Hill Book Co, New York
Oelze J (1983) Structure of phototrophic bacteria; development of the photosynthetic apparatus. In: Ormerod JG (ed) The phototrophic bacteria. Anaerobic life in the light. Blackwell Scientific Publications, Oxford, pp 8–34
Oelze J, Drews G (1972) Membranes of photosynthetic bacteria. Biochim Biophys Acta 265: 209–239
Pfennig N, Trüper HG (1974) The phototrophic bacteria. In: Buchanan RE, Gibbons NE (eds) Bergey's Manual of Determinative Bacteriology, 8th edn., Williams and Wilkins Co, Baltimore, pp 24–75
Pierson BK, Castenholz RW (1974) Studies of pigments and growth in Chloroflexus aurantiacus, a phototrophic filamentous bacterium. Arch Microbiol 100: 283–305
Remsen CC (1978) Comparative subcellular architecture of photosynthetic bacteria. In: Clayton RK, Sistrom WR (eds) The photosynthetic bacteria. Plenum Press, New York, pp 31–60
Reynolds ES (1963) The use of lead citrate at high pH as an electronopaque stain in electron microscopy. J Cell Biol 17: 208–212
Smith GI, Kamen MD (1970) Variable cellular composition of Chromatium in growing cultures. Arch Mikrobiol 73: 1–18
Sokal RR, Rohlf FJ (1981) Biometry. The principles and practice of statistics in biological research. WH Freeman and Co., San Francisco
Sprague SG, Staehelin LA, Di Bartolomeis MJ, Fuller RC (1981) Isolation and development of chlorosomes in the green bacterium Chloroflexus aurantiacus. J Bacteriol 147: 1021–1031
Stanier RY, Doudoroff M, Kunisawa R, Contopoulou R (1959) The role of organic substrates in bacterial photosynthesis. Proc Natl Acad Sci USA 45: 1246–1260
Trentini WC, Starr MP (1967) Growth and ultrastructure of Rhodomicrobium vannielli as a function of light intensity. J Bacteriol 93: 1699–1708
Trüper HG (1978) Sulfur metabolism. In: Clayton RK, Sistrom WR (eds) The photosynthetic bacteria. Plenum Press, New York, pp 677–687
Trüper HG, Schlegel HG (1964) Sulfur metabolism in Thiorhodaceae. I. Quantitative measurements on growing cells of Chromatium okenii. Antonie van Leeuwenhoek J Microbiol Serol 30: 225–238
Van Gemerden H (1968) On the ATP generation by Chromatium in darkness. Arch Mikrobiol 64: 118–124
Van Gemerden H (1980) Survival of Chromatium vinosum at low light intensities. Arch Microbiol 125: 115–121
Van Gemerden H, Beeftink HH (1983) Ecology of phototrophic bacteria. In: Ormerod JG (ed) The phototrophic bacteria: Anaerobic life in the light. Blackwell Scientific Publications, Oxford, pp 146–184
Van Gemerden H, Montesinos E, Mas J, Guerrero R (1985) Diel cycle of metabolism of phototrophic purple sulfur bacteria in Lake Cisó (Spain). Limnol Oceanogr 30: 932–943
Weible ER (1979) Stereological methods. Vol 1. Practical methods for biological morphometry. Academic Press, London
Zimmerman R, Meyer-Reil LA (1974) A new method for fluorescence staining of bacterial populations on membrane filters. Kieler Meeresforsch 30: 24–27
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Esteve, I., Montesinos, E., Mitchell, J.G. et al. A quantitative ultrastructural study of Chromatium minus in the bacterial layer of Lake Cisó (Spain). Arch. Microbiol. 153, 316–323 (1990). https://doi.org/10.1007/BF00248999
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DOI: https://doi.org/10.1007/BF00248999