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Plant Uncoupling Mitochondrial Protein Activity in Mitochondria Isolated from Tomatoes at Different Stages of Ripening

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Abstract

In the present study we have observed a higher state of coupling in respiring mitochondriaisolated from green as compared to red tomatoes (Lycopersicon esculentum, Mill.). Greentomato mitochondria produced a membrane potential (ΔΨ) high enough to phosphorylate ADP,whereas in red tomato mitochondria, BSA and ATP were required to restore ΔΨ to the levelof that obtained with green tomato mitochondria. This supports the notion that such uncouplingin red tomato mitochondria is mediated by a plant uncoupling mitochondrial protein (PUMP;cf. Vercesi et al., 1995). Nevertheless, mitochondria from both green and red tomatoes exhibitedan ATP-sensitive linoleic acid (LA)-induced ΔΨ decrease providing evidence that PUMP isalso present in green tomatoes. Indeed, proteoliposomes containing reconstituted green or redtomato PUMP showed LA uniport and LA-induced H+ transport. It is suggested that the higherconcentration of free fatty acids (PUMP substrates) in red tomatoes could explain the lowercoupling state in mitochondria isolated from these fruits.

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Author notes

  1. Iseli L. Nantes

    Present address: Centro Interdisciplinar de Investigação Bioquímica, Universidade de Mogi das Cruzes, CEP 08780-911, Mogi das Cruzes, SP, Brazil

Authors and Affiliations

  1. Department of Membrane Transport Biophysics, Institute of Physiology, Prague, Czech Republic

    Alexandre D. T. Costa & Petr Ježek

  2. Departamento de Patologia Clínica (NMCE), Faculdade de Ciências Meédicas, Universidade Estadual de Campinas, CEP, 13083-970, Campinas, SP, Brazil

    Iseli L. Nantes, Petr Ježek & Anibal E. Vercesi

  3. Centro de Biologia Molecular e Engenharia Gene;aatica, Universidade Estadual de Campinas, CEP 13083 - 970, Campinas, SP, Brazil

    Adílson Leite & Paulo Arruda

Authors
  1. Alexandre D. T. Costa
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  2. Iseli L. Nantes
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  3. Petr Ježek
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  4. Adílson Leite
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  5. Paulo Arruda
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  6. Anibal E. Vercesi
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Correspondence to Anibal E. Vercesi.

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Costa, A.D.T., Nantes, I.L., Ježek, P. et al. Plant Uncoupling Mitochondrial Protein Activity in Mitochondria Isolated from Tomatoes at Different Stages of Ripening. J Bioenerg Biomembr 31, 527–533 (1999). https://doi.org/10.1023/A:1005408809619

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