Summary
The influence of fungi isolated from perennial ryegrass roots on the germination and development of seedlings of perennial ryegrass was investigated. The basic procedure employed was to sterilise the seed surface and then inoculate with fungi and plant in non-sterile soil. It was realised that the fungal isolate inoculated on to the sterile seed surface would not remain dominant in the root region of the host and would have an influence on the host which would decline with time from when the seed germinated. This was because it would have to face antagonism from the normal components of the root microflora present in the non-sterile soil.Trichoderma viride delayed the emergence of the seedlings and reduced the production of herbage, an observation consistent with results of some other investigators. A sterile hyaline fungus stimulated the emergence of the seedlings, but subsequent tests showed that the presence of the microflora of the seed coat, or the soil microflora, or the sterile hyaline fungus was effective in promoting the rate of germination of seed that had been surface sterilised. Leaching seed in water brought about an increase in the rate of seed germination, and it is suggested that there might be a germination inhibitor soluble in water present in the seed coat, which might be inactivated by saprophytic micro-organisms.
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References
Brian, P. W. and Grove, J. F., Gibberellic acid. Endeavour16, 161–171 (1957).
Butler, F. C., Saprophytic behaviour of some cereal root-rot fungi. I. Saprophytic colonisation of wheat straw. Ann. Applied Biol.40, 284–287 (1953).
Chesters, C. G. C., and Assawah, M. W., Koch's postulates applied to the microecology of fungi inhabiting root surfaces. Nature (London)178, 1062–1063 (1956).
Davies, F. R., Superiority of silver nitrate over mercuric chloride for surface sterilisation in the isolation ofOphiobolus graminis Sacc. Canad. J. Research C13, 168–173 (1935).
Eaton, F. M. and Rigler, N. E., Influence of carbohydrate levels and root surface microfloras on Phymatotrichum root-rot in cotton and maize plants. J. Agr. Research72, 137–161 (1946).
Edwards, E. T., The biological antagonism ofGibberella fujikuroi andGibberella fujikuroi var subglutinans toTrichoderma viride, with notes on the pathological effects of the latter fungus on maize. J. Australian Inst. Agr. Sci.6, 51–100 (1940).
Evenari, M., Germination inhibitors. Botan. Rev.15, 153–194 (1949).
Garrett, S. D., Biology of Root-infecting Fungi. Cambridge University Press (1956).
Jensen, J. L., Bacterial treatment of non-leguminous seeds as an agricultural practice. Australian J. Sci.4, 117–120 (1942).
Simmonds, P. M. and Ledingham, R. J., A study of the fungus flora of wheat roots. Sci. Agr.18, 49–59 (1937).
Toole, E. H., Hendricks, S. B., Borthwick, H. A. and Toole, V. K., Physiology of seed germination. Ann. Rev. Plant Physiol.7, 299–324 (1956).
Tveit, M. and Wood, R. K. S., The control of Fusarium blight in oat seedlings with antagonistic species ofChaetomium. Ann. Applied Biol.43, 538–552 (1955).
Waid, J. S., Distribution of fungi within the decomposing tissues of ryegrass roots. Trans. Brit. Mycol. Soc.40, 391–406 (1957).
Waksman, S. A., Microbiol Antagonism and Antibiotic Substances. New York: Commonwealth Fund (1947).
Wood, R. K. S. and Tveit, M., Control of plant diseases by use of antagonistic organisms. Botan. Rev.21, 441–492 (1955).
Wright, J. M., Biological control of a soil-borne Pythium infection by seedling inoculation. Plant and Soil8, 132–140 (1959b).
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Humphreys Jones, D.R., Waid, J.S. Influence of fungal isolates on germination and growth of perennial ryegrass. Plant Soil 19, 139–150 (1963). https://doi.org/10.1007/BF01347868
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DOI: https://doi.org/10.1007/BF01347868