Abstract
Meiosis was examined in pollen mother cells of F1 hybrids made from crosses between wheat (Triticum aestivum) and lymegrass (Leymus arenarius and L. mollis). Fluorescence genomic in situ hybridization detected pairing between wheat and lymegrass chromosomes during prophase I and metaphase I. Such pairing, when resulting in bivalent formation, was likely to yield correct disjunction, and hence intergenomic recombination could be incorporated into the gametes. Bivalents in these hybrids, however, were more frequently formed between chromosomes of the same parental origin. Univalents were common, whereas multivalents were not clearly detected. Meiotic behaviour in some cells was not totally aberrant, and this may have accounted for the presence of normal pollen. The results are discussed in relation to intergenomic pairing, meiotic behaviour in wide-hybrids and genome relationships, including the Leymus genome origin.
Similar content being viewed by others
References
Anamthawat-Jónsson K, Schwarzacher T, Leitch AR, Bennett MD, Heslop-Harrison JS (1990) Discrimination between closely related Triticeae species using genomic DNA as probe. Theor Appl Genet 79: 721–728.
Anamthawat-Jónsson K, Reader S (1995) Pre-annealing of total genomic DNA probes for simultaneous genomic in situ hybridization. Genome 38: 814–816.
Anamthawat-Jónsson K, Bödvarsdóttir SK, Bragason BTh, Gudmundsson J, Martin PK, Koebner RMD (1997) Wide hybridization between wheat (Triticum L.) and lymegrass (Leymus Hochst.) Euphytica 93: 293–300.
Baum M, Lagudah ES, Appels R (1992) Wide crosses in cereals. Annu Rev Plant Physiol Plant Mol Biol 43: 117–143.
Blakeslee AF, Avery AG (1937) Methods of inducing doubling of chromosomes in plants. J Hered 28: 392–411.
Dewey RD (1984) The genomic system of classification as a guide to intergeneric hybridization with the perennial Triticeae. In: Gustafson JP, ed. Proceedings of the 16th Stadler Genetics Symposium. New York: Plenum Press, pp 209–279.
Fakuda K, Sakamoto S (1992) Studies on unreduced gamete formation in hybrids between tetraploid wheats and Aegilops squarrosa L. Hereditas 116: 253–255.
Gale MD, Miller TE (1987) The introduction of alien genetic variation in wheat. In: Lupton FGH, ed. Wheat Breeding: its Scientific Basis. London: Chapman & Hall, pp 173–210.
Hansen NJP, Andersen SB (1996) In vitro chromosome doubling potential of colchicine, oryzalin, trifluralin, and AMP in Brassica napus microspore culture. Euphytica 88: 159–164.
Heneen WH (1963) Cytology of the intergeneric hybrid Elymus arenarius × Secale cereale. Hereditas 49: 61–77.
Jauhar PP (1992) Chromosome pairing in hybrids between hexaploid bread wheat and tetraploid crested wheatgrass (Agropyron cristatum). Hereditas 116: 107–109.
Jauhar PP (1995) Morphological and cytological characteristics of some wheat × barley hybrids. Theor Appl Genet 90: 872–877.
Jiang J, Friebe B, Gill BS (1994) Recent advances in alien gene transfer in wheat. Euphytica 73: 199–212.
Jones GH (1987) Chiasmata. In: Moens PB, ed. Meiosis. Orlando: Academic Press, pp 213–244.
Jones GH, Vincent JE (1994) Meiosis in autopolyploid Crepis capillaris. II. Autotetraploids. Genome 37: 497–505.
Martinez M, Naranjo T, Cuadrado C, Romero C (1996) Synaptic behaviour of the tetraploid wheat Triticum timopheevii. Theor Appl Genet 93: 1139–1144.
Mello-Sampayo T (1971) Genetic regulation of meiotic chromosome pairing by chromosome 3D of Triticum aestivum. Nature New Biol 230: 22–23.
Nilsson NO, Sall T, Bengtson BO (1993) Chiasma and recombination data in plants: are they compatible? Trends Genet 9: 344–348.
Ockendon DJ, Gates PJ (1976) Reduced pollen viability in the onion (Allium cepa). New Phytol 76: 511–517.
Ørgaard M, Heslop-Harrison JS (1994) Investigations of genome relationships between Leymus, Psathyrostachys and Hordeum inferred by genomic DNA:DNA in situ hybridization. Ann Bot. 73: 195–203.
Pasakinskine I, Anamthawat-Jónsson K, Humphreys MW, Jones RN (1997) Novel diploids following chromosome elimination and somatic recombination in Lolium multiflorum × Festuca arundinacea hybrids. Heredity 78: 464–469.
Riley R, Chapman V (1958) Genetic control of the cytologically diploid behaviour of hexaploid wheat. Nature 182: 713–715.
Schertan H, Loidl J, Schuster T, Schweizer D (1992) Meiotic chromosome condensation and pairing in Saccharomyces cerevisiae studied by chromosome painting. Chromosoma 101: 590–595.
Schwarzacher T, Leitch AR (1994) Enzymatic treatment of plant material to spread chromosomes for in situ hybridization. In: Isaac PG, ed. Methods in Molecular Biology, Vol. 28. New Jersey: Humana Press, pp 153–160.
Schwarzacher T, Heslop-Harrison JS (1995) Molecular cytogenetic investigations of meiosis. In: Brandham PE, Bennett MD, eds. Kew Chromosome Conference IV. Kew: Royal Botanic Gardens, pp 407–416.
Schwarzacher T, Leitch AR, Bennett MD, Heslop-Harrison JS (1989) In situ localization of parental genomes in a wide hybrid. Ann Bot 64: 315–324.
Sybenga J (1996) Recombination and chiasmata: few but intriguing discrepancies. Genome 39: 473–484.
Thomas J, Chen Q, Howes N (1997) Chromosome doubling of haploids of common wheat with caffeine. Genome 40: 552–558.
Wallace AJ, Callow RS (1995a) Meiotic variation in an intergenomic autopolyploid series. I. Chiasma frequency. Genome 38: 122–132.
Wallace AJ, Callow RS (1995b) Meiotic variation in an intergenomic autopolyploid series. II. Pairing behaviour. Genome 38: 133–139.
Wang RRC, Hsiao C (1984) Morphology and cytology of interspecific hybrids of Leymus mollis. J Hered 75: 488–492.
Wang RRC, Jensen KB (1994) Absence of the J genome in Leymus species (Poaceae: Triticeae): evidence from DNA hybridization and meiotic pairing. Genome 37: 231–235.
Wilkinson MJ, Bennett ST, Clulow SA, Allainguillaume J, Harding K, Bennett MD (1995) Evidence for somatic translocation during potato dihaploid induction. Heredity 74: 146–152.
Weiner BM, Kleckner N (1994) Chromosome pairing via multiple interstitial interactions before and during meiosis in yeast. Cell 77: 977–991.
Zhang HB, Dvorak J (1991) The genome origin of tetraploid species of Leymus (Poaceae: Triticeae) inferred from variation in repeated nucleotide sequences. Am J Bot 78: 871–884.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Anamthawat-jónsson, K., Bödvarsdóttir, S.K. Meiosis of Wheat × Lymegrass Hybrids. Chromosome Res 6, 339–344 (1998). https://doi.org/10.1023/A:1009281911723
Issue Date:
DOI: https://doi.org/10.1023/A:1009281911723