Abstract
Streptomyces antibioticus Tü 6040 is the producer of simocyclinones, which belong to a novel family of angucyclinone antibiotics some of which show antitumor activities. Growth and antibiotic production is dependent on the medium composition, especially on the carbon and nitrogen source, and on the fermentation conditions. The best results with respect to antibiotic productivity were achieved using a chemically defined medium with glycerol and L-lysine as carbon and nitrogen source, respectively, in an airlift fermenter with minimised shear stress at low gas flow rates withour oxygen limitation. These conditions led to a homogeneous formation of pellets of 1–2 mm in diameter and guaranteed reproducible product yields of the main compound, simocyclinone D8, in the range of 300 mg/l.
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References
Asai T, Sawada H, Yamaguchi T, Suzuki M, Higashide E & Uchida M (1978) The effect of mechanical damage on the production of maridomycin by Streptomyces hygroscopicus. J. Ferm. Technol. 56: 374–379
Clark GJ, Langley D & Bushell ME (1995) Oxygen limitation can induce microbial secondary metabolite formation: investigations with miniature electrodes in shaker and bioreactor culture. Microbiology 144: 663–669
Dahod SK (1999) Raw materials selection and medium development for industrial fermentation processes. In: Demain AL & Davies JE (Eds) Manual of Industrial Microbiology and Biotechnology (pp 213–220). ASM Press, Washington
Dick O, Onken U, Sattler I & Zeeck A (1994) Influence of the increased dissolved oxygen concentration on productivity and selectivity in cultures of a colabomycin-producing strain of Streptomyces griseoflavus. Appl. Microbiol. Biotechnol. 41: 373–377
Fiechter A (1986) Bioprocess development. In: Vanek Z & Hostalek Z (Eds) Overproduction of Microbial Metabolites (pp 231–259). Butterworth Publishers, Stoneham
Hinman JW, Caron EL & Hoeksema H (1957) The structure of novobiocin. J. Am. Chem. Soc. 79: 3789–3800.
Hosobuschi M & Yoshikawa H (1999) Scale-up of microbial processes. In: Demain AL & Davies JE (Eds) Manual of Industrial Microbiology and Biotechnology (pp 236–239). ASM Press, Washington
Merchuk JC (1991) Shear effects on suspended cells. Adv. Biochem. Eng. 44: 65–95
Roman RV & Gavrilescu M (1994) Oxygen transfer efficiency in the biosynthesis of antibiotics in bioreactors with a modified Rushton turbine agitator. Acta Biotechnol. 14: 181–192
Posten CH & Cooney CL (1993) Growth of microorganisms. In: Rehm H-J & Reed G (Eds.) Biotechnology, Vol. 1 (pp 111–162). VCH Verlagsgesellschaft, Weinheim
Schimana J, Walker M, Zeeck A & Fiedler H-P (2000) Simocyclinones: diversity of metabolites is dependent on fermentation conditions. J. Industr. Microbiol. Biotechnol. (in press).
Sezaki M, Hara T, Ayukawa S, Takeuchi T, Okami Y, Hamada M, Nagatsu T & Umezawa H (1968) Studies on a new antibiotic pigment, aquayamycin. J. Antibiot. 21: 91–97
Strobel RJ & Sullivan GR (1999) Experimental design for improvement of fermentations. In: Demain AL and Davies JE (Eds) Manual of Industrial Microbiology and Biotechnology. (pp 80–93). ASM Press, Washington
Tamura S, Park Y, Toriyama M & Okabe M (1997) Change of mycelial morphology in tylosin production by batch culture of Streptomyces fradiae under various shear conditions. J. Ferm. Biotechnol. 83: 523–528
Tarbell DS, Carman RM, Chapman DD, Huffman KR & Mc Corkindale NJ (1960) The structure of fumagillin. J. Am. Chem. Soc. 82: 1005–1006
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Theobald, U., Schimana, J. & Fiedler, HP. Microbial growth and production kinetics of Streptomyces antibioticus Tü 6040. Antonie Van Leeuwenhoek 78, 307–313 (2000). https://doi.org/10.1023/A:1010282818272
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DOI: https://doi.org/10.1023/A:1010282818272