General cytotoxicity was assayed for ceramic (Al2O3, ZrO2/Y2O3, AIN, B4C, BN, SiC, Si3N4, TiB4, TiC, TiN) diamond and graphite powders, using 3T3 Balb/c permanent cell lines. Neutral red test was carried out in order to establish cell viability. Further investigations were undertaken on human differentiated cells (human umbilical venous endothelial cells): cell behaviour (MTT assay, total cell protein content) and differentiation (immunofluorescence) were studied. In both cases, no cytotoxic effect has been noticed. All the impurities contained at low concentration in these powders do not seem to present any effect. The correlation which has been previously observed between cytotoxicity-cell culture response and blood haemolysis for polymers has not been established here for ceramic powders. We conclude that all the ceramic powders tested here and therefore the corresponding bulk ceramics or ceramic coatings do not induce any cytotoxic effect.
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References
T. KANEMANRU, New Mater. Jpn, Jan (1990) 12.
A. BOLZ and M. SCHALDACH, Artificial Organs 14 (1990) 260.
I. DION, X. ROQUES, Ch. BAQUEY, E. BAUDET, B. BASSECATHALINAT and N. MORE, Biomed. Mater. Eng. 3 (1993) 51.
Y. MITAMURA, T. MIKAMI, T. YUTA, T. MATSUMOTO, T. SHIMOOKA, E. OKAMOTO, N. EIZUKA and K. YAMAGUCHI, Trans. Amer. Soc. Artificial Intern. Organs 22 (1986) 444.
I. DION, Ch. BAQUEY, B. CANDELON and J. R. MONTIES, Int. J. Artificial Organs 15 (9) (1992) 437.
C. J. KIRKPATRICK, Med. Device Technol. Sept–Oct. (1990) 38.
M. F. HARMAND, L. BORDENAVE, R. BAREILLE, A. NAJI, R. JEANDOT, F. ROUAIS and D. DUCASSOU, J. Biomater. Sci. Polymer. Ed. 2 (1991) 67.
I. DION, M. LAHAYE, R. SALMON, P. V. HUONG, L. TRUT, J. P. CAZORLA, F. ROUAIS, Ch. BAQUEY, J. R. MONTIES and P. HAVLIK, J. Mater. Sci. Mater. Med. (in press).
I. DION, M. LAHAYE, R. SALMON, Ch. BAQUEY, J. R. MONTIES and P. HAVLIK, Biomaterials 14 (2) (1993) 107.
E. A. JAFFE, R. L. NACHMAN, C. G. BECHER and C. R. MINICK, J. Clin. Invest. 52 (1973) 2745.
L. BORDENAVE, F. LEFEBVRE, R. BAREILLE, F. ROUAIS, Ch. BAQUEY and M. RABAUD, Biomaterials 13 (7) (1992) 439.
Association Française de Normalisation (AFNOR), Matériel médicochirurgical, biocompatibilité des implants chirurgicaux, Méthodes d'extraction, S93F, Doc. 12, PR S90 701 (1987).
C. R. PARISH and A. MULLBACHER, J. Immunol. Meth. 58 (1983) 225.
T. MOSMANN, J. Immunol. Meth. 65 (1983) 55.
V. H. LOWRY, N. J. ROSEBROUGH, A. L. FARR and R. J. RANDALL, J. Biol. Chem. 193 (1951) 265.
D. V. LINDLEY, in “Introduction to probability and statistics from a Baypesian viewpoint”, Part I, Probability (Cambridge University Press, 1965), p. 135.
D. SGOURAS and R. DUNCAN, J. Mater. Sci. Mater. Med. 1 (1990) 61.
A. NAJI, Thèse, Université de Bordeaux I, no 271 (1988).
E. O. DILLINGHAM, N. WEBB, W. H. LAWRENCE and J. AUTIAN, J. Biomed. Mater. Res. 9 (1975) 569.
ISOL/TR 7405 (1984) (F) Standard.
C. J. KIRKPATRICK and C. MITTERMAYER, J. Mater. Sci. Mater. Med. 1 (1990) 9.
A. NAJI and M. F. HARMAND, Biomaterials 12 (1991) 690.
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Dion, I., Bordenave, L., Lefebvre, F. et al. Physico-chemistry and cytotoxicity of ceramics. J Mater Sci: Mater Med 5, 18–24 (1994). https://doi.org/10.1007/BF00121148
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DOI: https://doi.org/10.1007/BF00121148