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Release of thioredoxin from Saccharomyces cerevisiae with environmental stimuli: solubilization of thioredoxin with ethanol

  • Applied Microbial and Cell Physiology
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Abstract

Thioredoxin is crucial for the maintenance of the redox status of cells of all types. Mammalian thioredoxin is secreted from various types of cells, although the mechanism underlying has not yet been clarified. Previously, we demonstrated that thioredoxin was released from Saccharomyces cerevisiae after treatment with ethanol. In this paper, we show that as well as ethanol, low-pH shock and hypoosmotic shock release thioredoxin. Low-molecular-weight proteins in yeast cells were preferentially released by treatment with ethanol and low-pH shock. A cell wall integrity pathway seems partially involved in the hypoosmotic shock-induced release of thioredoxin. Considerable amounts of thioredoxin were present in the insoluble fractions of the cells, a portion of which was associated with lipid microdomains that are resistant to nonionic detergent at 4°C. The intracellular localization of thioredoxin may influence the efficiency of its release from yeast cells with ethanol.

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Acknowledgements

We thank Drs. M. Hall and Y. Ohya for plasmids. We are also grateful to T. Yasuoka for his technical assistance.

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Authors and Affiliations

  1. Laboratory of Molecular Microbiology, Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Uji, Kyoto, 611-0011, Japan

    Yoko Takeuchi, Wataru Nomura, Takumi Ohdate, Shingo Izawa & Yoshiharu Inoue

  2. Redox Bioscience Inc., Shimotsutsumi-cho, Sakyo-ku, Kyoto, 606-8396, Japan

    Shogo Tamasu & Kazuo Murata

  3. Institute for Viral Research, Kyoto University, Kawahara-cho, Sakyo-ku, Kyoto, 606-4002, Japan

    Hiroshi Masutani & Junji Yodoi

Authors
  1. Yoko Takeuchi
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  2. Wataru Nomura
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  3. Takumi Ohdate
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  4. Shogo Tamasu
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  5. Hiroshi Masutani
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  6. Kazuo Murata
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  7. Shingo Izawa
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  8. Junji Yodoi
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  9. Yoshiharu Inoue
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Correspondence to Yoshiharu Inoue.

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Takeuchi, Y., Nomura, W., Ohdate, T. et al. Release of thioredoxin from Saccharomyces cerevisiae with environmental stimuli: solubilization of thioredoxin with ethanol. Appl Microbiol Biotechnol 75, 1393–1399 (2007). https://doi.org/10.1007/s00253-007-0949-1

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  • DOI: https://doi.org/10.1007/s00253-007-0949-1

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