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Investigation of the applicability of Zernike moments to the classification of SDS 2D-PAGE maps

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Abstract

The aim of this work is to investigate the performance of multivariate classification techniques like partial least squares–discriminant analysis (PLS-DA) and linear discriminant analysis (LDA) when using Zernike moments as global image descriptors, in the classification of sodium dodecyl sulphate (SDS) two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) maps affected by different levels of deformation. Synthetic sets of images simulating real SDS 2D-PAGE maps were analysed in controlled conditions to obtain information on the robustness and limits of applicability of the classification techniques operating on the basis of a given image decomposition method.

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Correspondence to Emilio Marengo.

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Marengo, E., Cocchi, M., Demartini, M. et al. Investigation of the applicability of Zernike moments to the classification of SDS 2D-PAGE maps. Anal Bioanal Chem 400, 1419–1431 (2011). https://doi.org/10.1007/s00216-011-4851-4

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  • DOI: https://doi.org/10.1007/s00216-011-4851-4

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