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Combining analytical approaches for better lignocellulosic biomass degradation: a way of improving fungal enzymatic cocktails?

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Abstract

Purpose

In this study, a combinatory approach was undertaken to assay the efficiency of fungal enzymatic cocktails from different fermentation conditions to degrade different lignocellulosic biomasses with the aim of finely characterizing fungal enzymatic cocktails.

Methods

Enzymatic assays (AZO and pNP-linked substrates and ABTS) were used to assess the composition of the fungal enzymatic cocktails for cellulase, xylanase and laccase activities. Comparisons were made with a new range of chromogenic substrates based on complex biomass (CBS substrates). The saccharification efficiency of the cocktails was evaluated as a quantification of the sugar monomers released from the different biomasses after incubation with the enzymatic cocktails.

Results

The results obtained showed striking differences between the AZO and pNP-linked substrates and the CBS substrates for the same enzymatic cocktails. On AZO and pNP-linked substrates, different hydrolysis profiles were observed between the different fungi species with Aspergillus oryzae being the most efficient. However, the results on CBS substrates were more contrasted depending on the biomass tested. Altogether, the results highlighted that assessing laccase activities and taking into account the complexity of the biomass to degrade were key in order to provide the best enzymatic cocktails.

Conclusion

The complementary experiments performed in this study showed that different approaches needed to be taken in order to accurately assess the ability of an enzymatic cocktail to be efficient when it comes to lignocellulosic biomass degradation. The saccharification assay proved to be essential to validate the data obtained from both simple and complex substrates.

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source as a measure of Carbon Catabolite Repression. Results are displayed as absorbances ± the standard deviation around the mean of three biological replicates

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Acknowledgements

This work was supported by the Alibiotech project that is financed by the European Union, the French State and the French Region of Hauts-de-France. The REALCAT platform is benefiting from a Governmental subvention administrated by the French National Research Agency (ANR) within the frame of the ‘Future Investments’ program (PIA), with the contractual reference ‘ANR-11-EQPX-0037’. The Hauts-de-France Region and the FEDER as well as the Centrale Innovation Fondation are thanked for their financial contribution to the acquisition of the equipment of the platform.

Funding

This study were funded by région hauts-de-france, feder and centrale innovation fondation.

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Authors and Affiliations

  1. UMRT BioEcoAgro 1158, Univ. Lille, INRAE, Univ. Liège, UPJV, JUNIA, Univ. Artois, Univ. Littoral Côte d’Opale, ICV – Institut Charles Viollette, 59000, Lille, France

    Roxane Raulo, Egon Heuson, Renato Froidevaux & Vincent Phalip

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  1. Roxane Raulo
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  2. Egon Heuson
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  3. Renato Froidevaux
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  4. Vincent Phalip
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Correspondence to Vincent Phalip.

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Raulo, R., Heuson, E., Froidevaux, R. et al. Combining analytical approaches for better lignocellulosic biomass degradation: a way of improving fungal enzymatic cocktails?. Biotechnol Lett 43, 2283–2298 (2021). https://doi.org/10.1007/s10529-021-03201-2

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  • DOI: https://doi.org/10.1007/s10529-021-03201-2

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