Within the framework of economic globalisation, ports serve as critical junctures in international trade and play a vital role. However, as infrastructure is closely linked to the natural environment, ports are highly susceptible to the impacts of meteorological disasters. Therefore, a comprehensive assessment of the risks posed by meteorological hazards to ports, establishing corresponding early warning mechanisms, and adopting reasonable response and recovery strategies, is paramount in ensuring the safe operation of ports and maintaining the stability of international trade. This study has comprehensively analysed historical data and identified the pre-established loss stratification system, improving the theoretical construct of “expected loss”. Additionally, this research has innovatively integrated the idea of preventative factors aligned with risk indicators. A quantitative algorithm was used to factor in the preventative factors within the computational procedure, deriving the weights pertinent to each risk indicator. This research aimed to reduce the subjectivity inherent in the weighting assignment process through such an approach, thereby enhancing disaster risk assessment’s scientific rigour and reliability. Moreover, it underscores the critical role of adaptive urban planning in enhancing the resilience of crucial economic nodes like ports, thereby contributing to the broader objectives of sustainable urban development. Full article

This study evaluates the effects of dietary Clostridium butyricum on growth performance and intestinal health in triploid rainbow trout (Oncorhynchus mykiss). Administered in a 12-week trial, five isonitrogenous and isolipidic feeds contained different C. butyricum levels [G1 (0), G2 (1.6 × 10⁶ CFU/g), G3 (1.2 × 10⁷ CFU/g), G4 (1.1 × 10⁸ CFU/g), and G5 (1.3 × 10⁹ CFU/g)]. Significant enhancements in growth performance, including improved feed conversion ratios and specific growth rates in the G4 group, were indicative of enhanced nutrient utilization, corroborated by optimal digestive enzyme activity levels. Antioxidant capabilities were also enhanced in the G4 group, indicated by increased serum superoxide dismutase and catalase activities, along with a significant decrease in malondialdehyde levels. Gut microbiota analysis indicated a probiotic concentration-dependent modulation of microbial communities, with a marked enrichment in beneficial bacterial phyla like Firmicutes in the G4 group. This microbial shift correlated with significant downregulations in immune-related gene expressions, including interleukins (IL-1β, IL-8), NF-κB, MyD88, and TNF-α, highlighting an activated immune response. Correspondingly, serum lysozyme and immunoglobulin M contents were significantly elevated in the G4 group. Challenge tests with Aeromonas salmonicida exhibited the higher disease resistance of fish fed the G4 diet. In conclusion, the study shows the potential of dietary C. butyricum, especially at 1.1 × 10⁸ CFU/g, in enhancing the growth, health, and disease resistance of triploid rainbow trout through modulating gut microflora and stimulating immune responses. Full article

The low availability of phosphorus has become a common problem worldwide. Phosphorus is essential for phenotypic morphology and ginsenoside synthesis. However, the effects of Pi stress on ginseng phenotype and ginsenoside synthesis remain unclear. Phenotypic analyses and transcriptomics revealed the phenotypic construction and regulation of differential genes involved in the physiological metabolism of ginseng under low-Pi stress. Root length and stem length were found to be significantly inhibited by phosphate-deficiency stress in the half-phosphate (HP) and no-phosphate (NP) treatment groups; however, the number of fibrous roots, which are regulated by phytohormones, was found to increase. In ginseng leaves, the indexes of physiological stress, superoxide anion (221.19 nmol/g) and malonaldehyde (MDA) (0.05 μmol/min/g), reached the maximum level. Moreover, chlorophyll fluorescence images and chlorophyll content further confirmed the inhibition of ginseng photosynthesis under low-Pi stress. A total of 579 and 210 differentially expressed genes (DEGs) were shared between NP and total phosphate (TP) and HP and TP, respectively, and only 64 common DEGs were found based on the two comparisons. These DEGs were mainly related to the synthesis of phosphate transporters (PHTs), phytohormones, and ginsenosides. According to KEGG analyses, four DEGs (Pg_s 0368.2, Pg_s3418.1, Pg_s5392.5 and Pg_s3342.1) affected acetyl-CoA production by regulating glycometabolism and tricarboxylic acid cycle (TCA). In addition, related genes, including those encoding 13 PHTs, 15 phytohormones, and 20 ginsenoside synthetases, were screened in ginseng roots under Pi-deficiency stress. These results indicate that changes in the ginseng phenotype and transcriptional regulation of DEGs are involved in the Pi-deficiency stress environment of ginseng, thereby providing new information regarding the development of ginseng for low-Pi tolerance. Full article

Spring frost is a perennial and widespread problem across many cool climatic and high-elevation winegrowing regions of the world. Vitis vinifera L. cv. Pinot noir is an early budding cultivar; thus, it is particularly susceptible to late-spring frost damage. In late April 2022, an advective frost event occurred throughout Western Oregon winegrowing regions and subsequently damaged a substantial number of commercial vineyards. Growers often are unsure of how to manage grapevines after a frost event. Limited research has shown little-to-no effect of pruning vs. non-pruning strategies on vine yield and productivity. In addition, pruning a frost-affected vineyard incurs additional labor costs that may offset the cost–benefit balance for the grower. Therefore, in this experiment, the effect of two different post-frost pruning treatments (cane pruning and spur pruning) on vine yield, berry composition, and vine vegetative growth were tested. No effect of post-frost pruning treatments on vine yield, berry composition, and vine vegetative characteristics was observed. Cluster numbers, cluster weights, and berries per cluster only differed between cane- vs. spur-pruned vines. Therefore, leaving frost-affected vines alone and a scaled-back vineyard management practice could be practical for economic reasons. Full article

Origanum vulgare L. is ethnomedicinally valuable against various diseases. In Romania, attention for the oregano extracts such as infusions, decoctions, or tinctures, which are very popular among consumers, is constantly increasing, mainly as an important therapeutic alternative. Therefore, this study was undertaken to evaluate the comparative cytotoxic and genotoxic effects of aqueous and hydroalcoholic extracts of local oregano using a sustainable method such as the Allium cepa assay. Two aqueous oregano extracts obtained by infusion (I01) and decoction (D02) and two hydroalcoholic extracts (E03—water/ethanol 80:20 v/v; E04—water/ethanol 60:40 v/v) were used in this study. Before performing the Allium cepa test, a phytochemical screening carried out using fast and efficient analytical methods (electrometry, colorimetry, UV-Vis spectrometry, and high-performance thin-layer chromatography/HPTLC) allowed the qualitative differences in the chemical profile of the investigated oregano extracts to be highlighted. The aqueous and hydroalcoholic oregano extracts were tested on root meristems of Allium cepa and the cytotoxicity and genotoxicity parameters evaluated were the mitotic index (MI) and chromosomal aberration (CA). The results revealed a decrease in MI for each analyzed sample, with hydroethanolic extract E04 showing the most significant effect on MI (9.66%, 3 times less than that of the control sample), followed by the D02 sample obtained by decoction. Chromosomal aberrations such as the ana-telophase with bridges, expelled chromosomes, or delayed chromosomes were observed in all four extracts. The frequency of cells with CA was higher in the case of samples treated with hydroalcoholic extracts compared to aqueous extracts. The experimental extraction conditions influenced the mitotic index, the varieties of identified chromosomal aberrations, and their frequency. Therefore, based on the result obtained in this study, it may be concluded that the Oregano vulgare L. extracts present cytotoxic and genotoxic effects on onion cells. The Allium cepa assay proves to be an easy-to-handle method, with reliable results, minimal cost, and environmental friendliness for the evaluation of the cytotoxic and genotoxic effects of oregano extracts. Full article

Jatropha seed cake (JSC) derived from Jatropha curcas seeds is a by-product of biodiesel production and, due to its high protein content, has been considered as a potential animal feed ingredient. However, the presence of toxic compounds such as phorbol esters and other anti-nutritional factors limits its use in animal feeding. Several detoxification approaches have been used to tackle these constraints and this review aims to summarize the recent advances in JSC treatment aiming to enhance its potential as an animal feedstuff. The review first provides an overview of the structure and composition of phorbol esters and other anti-nutritional compounds, discussing its toxic effects on different animal species. It then explores several detoxification methodologies giving special emphasis to its effects on the nutritional composition of JSC and on the use of the treated substrate as a feed ingredient in fish, poultry, pigs, and ruminants, highlighting their growth performance, nutrient utilization, and animal health issues. Overall, the review concludes that these treatments hold great potential for the detoxification and utilization of JSC as an animal feed ingredient. However, further research is needed to optimize the treatment conditions, evaluate the economic feasibility, and assess the long-term effects of treated JSC on animal health and product quality. Full article

This study examines stock market response (SMR) to the Japanese tourism industry (TI) after the government’s announcement of travel subsidies (TRSs) during the COVID-19 pandemic in 2020, using a sample comprising 80 listed Japanese firms in the TI and an event study method (ESM) to determine the impact of government policy responses (GPRs) to the pandemic. This study found that investors in the TI reacted positively to the announcement of subsidies; this positive effect persisted for 50 trading days after the announcement but was weaker for transportation firms. The results suggest that TRSs are important for the TI, with a stronger link to travel-related firms, such as airlines and travel agencies, hotels, and amusement services. However, investors in the TI reacted negatively to policies that directly addressed the pandemic, such as social distance policies (SDPs). These results are robustly confirmed when we measure abnormal returns by using a three-factor model. The results offer useful insights for policymakers and practitioners aiming to mitigate economic loss from disasters such as the COVID-19 pandemic. Full article

Retinal vessel segmentation plays a crucial role in medical image analysis, aiding ophthalmologists in disease diagnosis, monitoring, and treatment guidance. However, due to the complex boundary structure and rich texture features in retinal blood vessel images, existing methods have challenges in the accurate segmentation of blood vessel boundaries. In this study, we propose the texture-driven Swin-UNet with enhanced boundary-wise perception. Firstly, we designed a Cross-level Texture Complementary Module (CTCM) to fuse feature maps at different scales during the encoding stage, thereby recovering detailed features lost in the downsampling process. Additionally, we introduced a Pixel-wise Texture Swin Block (PT Swin Block) to improve the model’s ability to localize vessel boundary and contour information. Finally, we introduced an improved Hausdorff distance loss function to further enhance the accuracy of vessel boundary segmentation. The proposed method was evaluated on the DRIVE and CHASEDB1 datasets, and the experimental results demonstrate that our model obtained superior performance in terms of Accuracy (ACC), Sensitivity (SE), Specificity (SP), and F1 score (F1), and the accuracy of vessel boundary segmentation was significantly improved. Full article

Cell therapy has proven to be a promising treatment for a range of neurological disorders, including Parkinson Disease, drug-resistant epilepsy, and stroke, by restoring function after brain damage. Nevertheless, evaluating the true effectiveness of these therapeutic interventions requires a deep understanding of the functional integration of grafted cells into existing neural networks. This review explores a powerful arsenal of molecular techniques revolutionizing our ability to unveil functional integration of grafted cells within the host brain. From precise manipulation of neuronal activity to pinpoint the functional contribution of transplanted cells by using opto- and chemo-genetics, to real-time monitoring of neuronal dynamics shedding light on functional connectivity within the reconstructed circuits by using genetically encoded (calcium) indicators in vivo. Finally, structural reconstruction and mapping communication pathways between grafted and host neurons can be achieved by monosynaptic tracing with viral vectors. The cutting-edge toolbox presented here holds immense promise for elucidating the impact of cell therapy on neural circuitry and guiding the development of more effective treatments for neurological disorders. Full article

Quantitative neuromuscular monitoring, as extolled by clinical guidelines, is advocated to circumvent the complications associated with neuromuscular blockers (NMBs), such as residual neuromuscular block (rNMB). Nonetheless, the worldwide utilization of such methods remains undesirable. Phonomyography (PMG) boasts the advantages of convenience, stability, and multi-muscle recording which may be a promising monitoring method. The purpose of this preliminary study is conducting a feasibility analysis and an effectiveness evaluation of a PMG prototype under general anesthesia. A prospective observational preliminary study was conducted. Twenty-five adults who had undergone none-cardiac elective surgery were enrolled. The PMG prototype and TOF-Watch SX simultaneously recorded the pharmacodynamic properties of single bolus rocuronium at the ipsilateral adductor pollicis for each patient. For the primary outcome, the time duration to 0.9 TOF ratio of the two devices reached no statistical significance (p > 0.05). For secondary outcomes, the multi-temporal neuromuscular-monitoring measurements between the two devices also reached no statistical significance (p > 0.05). What is more, both the Spearman’s and Pearson’s correlation tests revealed a strong correlation across all monitoring periods between the PMG prototype and TOF-Watch SX. Additionally, Bland–Altman plots demonstrated a good agreement between the two devices. Thus, the PMG prototype was a feasible, secure, and effective neuromuscular-monitoring technique during general anesthesia and was interchangeable with TOF-Watch SX. Full article

The high prevalence of postpartum depression makes it necessary for midwives and nurses to implement prenatal interventions for expectant mothers. The current study aims to investigate and highlight the importance of the digital training of nurses in order to help women mitigate the symptoms of postpartum depression and protect infants. To approach this, we conducted a bibliometric analysis to address the study’s main objective. Articles were retrieved from the Scopus database for the timeframe 2000–2023. Data analysis was conducted using the statistical programming language R (version R-4.4.) and the bibliometric software VOSviewer (version 1.6.20) and Biblioshiny (version 4.1.4), focused on year, journal, and country. For this investigation, we selected a total of 31 MeSH keywords and sub-headings that exhibited significant frequencies. We consistently used six significant clusters of MeSH keywords. We obtained a total of 585 articles from the Scopus database that were major contributors to the field of PPD, as evidenced by their extensive publication of research articles and their influential role in the domain. The studies included a thorough analysis of depression research, the use of scales for diagnosing and screening PPD, psychological studies related to PPD, and the exploration of causes, mechanisms, outcomes, and genetic factors. Our study’s results demonstrate a steady and significant increase in the availability of information on PPD. Importantly, the novelty of the current study lies in highlighting the need for a transition in the ways in which nurses and midwives are trained to mitigate postpartum disease by integrating emerging technologies into their practices. The knowledge provided here has the potential to serve as a foundation for future advancements in obstetric psychology, both presently and in the future. Full article

Human papillomavirus (HPV)-related oropharyngeal squamous cell carcinoma (OPSCC) presents unique challenges and opportunities for treatment, particularly regarding de-escalation strategies to reduce treatment morbidity without compromising oncological outcomes. This paper examines the role of Transoral Robotic Surgery (TORS) as a de-escalation strategy in managing HPV-related OPSCC. We conducted a comprehensive literature review from January 2010 to June 2023, focusing on studies exploring TORS outcomes in patients with HPV-positive OPSCC. These findings highlight TORS’s potential to reduce the need for adjuvant therapy, thereby minimizing treatment-related side effects while maintaining high rates of oncological control. TORS offers advantages such as precise tumor resection and the ability to obtain accurate pathological staging, which can guide the tailoring of adjuvant treatments. Some clinical trials provide evidence supporting the use of TORS in specific patient populations. The MC1273 trial demonstrated promising outcomes with lower doses of adjuvant radiotherapy (RT) following TORS, showing high locoregional tumor control rates and favorable survival outcomes with minimal side effects. ECOG 3311 evaluated upfront TORS followed by histopathologically directed adjuvant therapy, revealing good oncological and functional outcomes, particularly in intermediate-risk patients. The SIRS trial emphasized the benefits of upfront surgery with neck dissection followed by de-escalated RT in patients with favorable survival and excellent functional outcomes. At the same time, the PATHOS trial examined the impact of risk-adapted adjuvant treatment on functional outcomes and survival. The ongoing ADEPT trial investigates reduced-dose adjuvant RT, and the DART-HPV study aims to compare standard adjuvant chemoradiotherapy (CRT) with a reduced dose of adjuvant RT in HPV-positive OPSCC patients. These trials collectively underscore the potential of TORS in facilitating treatment de-escalation while maintaining favorable oncological and functional outcomes in selected patients with HPV-related OPSCC. The aim of this scoping review is to discuss the challenges of risk stratification, the importance of HPV status determination, and the implications of smoking on treatment outcomes. It also explores the evolving criteria for adjuvant therapy following TORS, focusing on reducing radiation dosage and volume without compromising treatment efficacy. In conclusion, TORS emerges as a viable upfront treatment option for carefully selected patients with HPV-positive OPSCC, offering a pathway toward treatment de-escalation. However, selecting the optimal candidate for TORS-based de-escalation strategies is crucial to fully leverage the benefits of treatment de-intensification. Full article

Subgradient methods are frequently used for optimization problems. However, subgradient techniques are characterized by slow convergence for minimizing ravine convex functions. To accelerate subgradient methods, special linear non-orthogonal transformations of the original space are used. This paper provides an overview of these transformations based on Shor’s original idea. Two one-rank linear transformations of Euclidean space are considered. These simple transformations form the basis of variable metric methods for convex minimization that have a natural geometric interpretation in the transformed space. Along with the space transformation, a search direction and a corresponding step size must be defined. Subgradient Fejer-type methods are analyzed to minimize convex functions, and Polyak step size is used for problems with a known optimal objective value. Convergence theorems are provided together with the results of numerical experiments. Directions for future research are discussed. Full article

Solar-terrestrial interaction is a dynamic process that manifests itself in the ionosphere. Interplanetary (IP) shocks or solar wind dynamic pressure pulses can generate enhanced brightening in dayside aurora. Foreshock transients are capable of inducing pressure changes, larger in magnitude than solar wind pressure pulses, which also contribute to intensifying dayside aurora. These pressure variations can accelerate particles into the ionosphere, generating field-aligned currents that produce magnetic impulse events and enhanced dayside auroral activity with periods of increased brightening. This study presents several dayside auroral brightening events that are not associated with IP shocks or solar wind dynamic pressure pulses. The dayside auroral brightening events are associated with a green (557.7 nm) to red (630.0 nm) ratio which is greater than 15. These extreme brightening events (EBEs) begin on the eastern or western end of a pre-existing dayside auroral arc. Periodic pulses of enhanced brightening are correlated with large sharp increases in the X-component (points toward the north-geographic pole) from ground magnetometers in the IMAGE network. EBEs occur predominately before magnetic noon and with X-component signatures from high-latitude stations. Ground-based data were obtained from the Kjell Henriksen Observatory in Longyearbyen and the IMAGE magnetometer network. Full article

Aspect-based sentiment analysis (ABSA), which aims to extract aspects and corresponding opinions from sentences and determine aspect sentiment polarity, has been widely studied in recent years. Most approaches focus on the subtasks of ABSA and deal with them in the pipeline method or end-to-end method. However, these approaches ignore the semantic information of the labels and the correlation between the labels. In this study, we process various ABSA tasks in a unified generative framework and use instruction prompts to guide the generative model to learn the relationships between different sentiment elements, accurately identify the sentiment elements in sentences, and improve the performance of the model in few-shot learning. Experimental results on several benchmark datasets show that our approach achieves significant performance gains. Among them, for the aspect term extraction and sentiment classification task on the Laptop 14 dataset, our method improves the F1 score by 4.08% and 1.87% on fully supervised learning compared to the GAS model and PARA model, respectively. In few-shot learning, we can achieve 80% of the fully supervised learning performance using one-tenth of the dataset. Our method can effectively address the problem of data shortage in low-resource environments. Full article

This work focuses on the efficient quantization of convolutional neural networks (CNNs). Specifically, we introduce a method called non-uniform uniform quantization (NUUQ), a novel quantization methodology that combines the benefits of non-uniform quantization, such as high compression levels, with the advantages of uniform quantization, which enables an efficient implementation in fixed-point hardware. NUUQ is based on decoupling the quantization levels from the number of bits. This decoupling allows for a trade-off between the spatial and temporal complexity of the implementation, which can be leveraged to further reduce the spatial complexity of the CNN, without a significant performance loss. Additionally, we explore different quantization configurations and address typical use cases. The NUUQ algorithm demonstrates the capability to achieve compression levels equivalent to 2 bits without an accuracy loss and even levels equivalent to ∼1.58 bits, but with a loss in performance of only ∼0.6%. Full article

Recently, the role of trace elements in the pathophysiology of obesity, insulin resistance (IR), and metabolic diseases has been explored. In this cross-sectional study, we aimed to assess the association of overweight, obesity, and cardiometabolic traits with serum copper (Cu) levels in 346 Mexican adults. Serum Cu level was measured by inductively coupled plasma mass spectrometry (ICP-MS). Anthropometrical data were collected, and biochemical parameters were measured. The triglyceride-glucose (TyG) index was used as a surrogate marker to evaluate IR. Overweight and obesity status was positively associated with the serum Cu level (β = 19.434 ± 7.309, p = 0.008). Serum Cu level was observed to have a positive association with serum triglycerides level (β = 0.160 ± 0.045, p < 0.001) and TyG (β = 0.001 ± 0.001, p < 0.001). Additionally, high serum Cu level was positively associated with overweight and obesity status (odds ratio [OR] = 1.9, 95% confidence interval [95% CI] 1.1–3.4, p = 0.014), hypertriglyceridemia (OR = 3.0, 95% CI 1.7–5.3, p < 0.001), and IR (OR = 2.6, 95% CI 1.4–4.6, p = 0.001). In conclusion, our results suggest that overweight, obesity, hypertriglyceridemia, and IR are positively associated with serum Cu levels in Mexican adults. Full article

Nano-adjuvant vaccines could induce immune responses and enhance immunogenicity. However, the application and manufacturing of nano-adjuvant is hampered by its challenging scale-up, poor reproducibility, and low security. Therefore, the present study aimed to optimize the preparation nanoparticles (NPs) using FDA-approved biopolymer materials poly(lactic acid) (PLA) and cationic lipid didodecyl-dimethyl-ammonium bromide (DDAB), develop the scale-up process, and evaluate the stability and biosafety of it. The optimum preparation conditions of DDAB/PLA NPs on a small scale were as follows: DDAB amount of 30 mg, aqueous phase volume of 90 mL, stirring rate at 550 rpm, and solidifying time of 12 h. Under the optimum conditions, the size of the NPs was about 170 nm. In scale-up preparation experiments, the vacuum rotary evaporation of 6 h and the Tangential flow ultrafiltration (TFU) method were the optimum conditions. The results suggested that DDAB/PLA NPs exhibited a uniform particle size distribution, with an average size of 150.3 ± 10.4 nm and a narrow polydispersity index (PDI) of 0.090 ± 0.13, coupled with a high antigen loading capacity of 85.4 ± 4.0%. In addition, the DDAB/PLA NPs can be stored stably for 30 days and do not have side effects caused by residual solvents. For biosafety, the acute toxicity experiments showed good tolerance of the vaccine formulation even at a high adjuvant dose. The local irritation experiment demonstrated the reversibility of muscular irritation, and the repeated toxicity experiment revealed no significant necrosis or severe lesions in mice injected with the high-dose vaccine formulation. Overall, the DDAB/PLA NPs exhibit potential for clinical translation as a safe candidate vaccine adjuvant. Full article

Background: Since metabolic diseases and atherosclerotic vascular events are firmly associated, herein we investigate changes in central microcirculation and atherosclerosis-related body fat distribution in patients with type 2 diabetes mellitus and obesity. Methods: Resting brain perfusion single-photon emission computed tomography (SPECT) imaging with Technetium-99m hexamethylpropylene amine oxime ([^99mTc]Tc-HMPAO SPECT) was performed, and the breath-holding index (BHI) and carotid intima-media thickness (cIMT) were measured to characterise central microcirculation. Besides CT-based abdominal fat tissue segmentation, C-peptide level, glycaemic and anthropometric parameters were registered to search for correlations with cerebral blood flow and vasoreactivity. Results: Although no significant difference was found between the resting cerebral perfusion of the two patient cohorts, a greater blood flow increase was experienced in the obese after the breath-holding test than in the diabetics (p < 0.05). A significant positive correlation was encountered between resting and provocation-triggered brain perfusion and C-peptide levels (p < 0.005). BMI and cIMT were negatively correlated (rho = −0.27 and −0.23 for maximum and mean cIMT, respectively), while BMI and BHI showed a positive association (rho = 0.31 and rho = 0.29 for maximum and mean BHI, respectively), which could be explained by BMI-dependent changes in fat tissue distribution. cIMT demonstrated a disproportional relationship with increasing age, and higher cIMT values were observed for the men. Conclusions: Overall, C-peptide levels and circulatory parameters seem to be strong applicants to predict brain microvascular alterations and related cognitive decline in such patient populations. Full article

This paper aims to discuss the microvascular reconstruction of the orbit and adjacent regions and to propose a checklist to aid the reconstructive surgeon in this challenging undertaking. The discussion is based on a literature review that includes 32 retrospective case series, 5 case reports published in the last 34 years in PubMed, and 3 textbook chapters. Additionally, it relies on the senior author’s expertise, described in a case series, and two case reports published elsewhere. Classifications and treatment algorithms on microvascular orbit reconstruction generally disregard patient-related factors. A more holistic approach can be advantageous: patient-related factors, such as age, comorbidities, prognosis, previous interventions, radiotherapy, and the wish for maximal dental rehabilitation and a prosthetic eye, have the same importance as defect-related considerations and can inform the choice of a reconstructive option. In this manuscript, we examine defect- and patient-related factors and new technologies, provide a checklist, and examine future directions. The checklist is intended as a tool to aid in the decision-making process when reconstructing the orbital region with microvascular flaps. Full article

An insect neuroactive helix ring peptide called U₁₁-MYRTX-Tb1a (abbreviated as U₁₁) from the venom of the ant, Tetramorium bicarinatum. U₁₁ is a 34-amino-acid peptide that is claimed to be one of the most paralytic peptides ever reported from ant venoms acting against blowflies and honeybees. The peptide features a compact triangular ring helix structure stabilized by a single disulfide bond, which is a unique three-dimensional scaffold among animal venoms. Pharmacological assays using Drosophila S2 cells have demonstrated that U₁₁ is not cytotoxic but instead suggest that it may modulate potassium channels via the presence of a functional dyad. In our work described here, we have tested this hypothesis by investigating the action of synthetically made U₁₁ on a wide array of voltage-gated K and Na channels since it is well known that these channels play a crucial role in the phenomenon of paralysis. Using the Xenopus laevis oocyte heterologous expression system and voltage clamp, our results have not shown any modulatory effect of 1 μM U₁₁ on the activity of Kv1.1, Kv1.3, Kv1.4, Kv1.5, Shaker IR, Kv4.2, Kv7.1, Kv10.1, Kv11.1 and KQT1, nor on DmNav and BgNav. Instead, 10 μM U₁₁ caused a quick and irreversible cytolytic effect, identical to the cytotoxic effect caused by Apis mellifera venom, which indicates that U₁₁ can act as a pore-forming peptide. Interestingly, the paralytic dose (PD₅₀) on blowflies and honeybees corresponds with the concentration at which U₁₁ displays clear pore-forming activity. In conclusion, our results indicate that the insecticidal and paralytic effects caused by U₁₁ may be explained by the putative pore formation of the peptide. Full article

Service-oriented architectures have become increasingly prevalent in internet application design, leading to a proliferation of services. Managing and composing these web services pose a classic non-deterministic polynomial-time hard problem. The web service composition problem (WSCP) based on the input–output model (IOM) involves the automatic composition of services without relying on a pre-defined workflow. Multi-objective evolutionary algorithms offer a solution that goes beyond simple weighted average calculations for quality of service, catering to diverse user needs in service composition. This paper introduces a multi-objective heuristic algorithm based on the SPEA2 called MSPEA2+. Specific modifications are incorporated in MSPEA2+ to support the breeding, crossover, and mutation processes, tailored for IOM-based WSCP. Validating the algorithm performance through finding suitable combination results for each task in the WSC-08 dataset, our experiments showed that MSPEA2+ has better iterative efficiency than existing multi-objective methods and outperforms them in terms of quality of service. MSPEA2+ achieved the lowest IGD value across all seven tasks, an improvement of 81.7% IGD value compared with the SPEA2 algorithm in WSC-0804. Full article