This paper introduces a new method for compressing images in energy-starved systems, like satellites, unmanned aerial vehicles, and Internet of Things nodes, which is based on coordinated group signal transformation (CGST). The transformation algorithm is a type of difference coding and may be classified as a non-transform-based image-compression method. CGST simplifies the difference signal conversion scheme using a single group codec for all signals. It considers color channels as correlated signals of a multi-channel communication system. The performance of CGST was evaluated using a dataset of 128 × 128 pixel images from satellite remote sensing systems. To adapt CGST to image compression, some modifications were introduced to the algorithm, such as fixing the procedure of the difference signals calculation to prevent any “zeroing” of brightness and supplementing the group codec with a neural network to improve the quality of restored images. The following types of neural networks were considered: fully connected, recurrent, convolution, and convolution in the Fourier space. Based on the simulation results, fully connected neural networks are recommended if the goal is to minimize processing delay time. These networks have a response time of 13 ms. Conversely, suppose the priority is to improve quality in cases where delays are not critical. In that case, convolution neural networks in the Fourier space should be used, providing an image compression ratio of 4.8 with better minimum square error and Mikowsky norm values than JPEG with the same compression ratio. Full article

Histocompatibility testing is pivotal in any renal transplantation workup, aimed at enhancing prospective donor recipient compatibility and improving transplant outcomes. The evolution and advancement of histocompatibility testing, particularly HLA typing, have significantly improved its precision. This study outlines the historical progression from serologic to DNA-based HLA typing, emphasizing the role of HLA proteins in immune response. Anti-HLA antibodies, targeting HLA proteins, pose challenges in renal transplantation. Monitoring and managing these antibodies are critical for renal transplant success. Complement-dependent cytotoxicity crossmatch and flow cytometry crossmatch are essential techniques for assessing donor–recipient compatibility. Panel-reactive antibody assesses antibodies against a panel of donor antigens, often HLA. Higher PRA levels (percentage) complicate donor matching, requiring specialized protocols. Virtual crossmatch evaluates recipient anti-HLA antibodies against potential donors through synthetic beads. This approach predicts crossmatch outcomes by comparing antibody profiles, offering a valuable tool for the risk assessment of renal transplantation. Despite advancements, a comprehensive understanding of alloreactive immune responses requires a combination of assays, emphasizing the importance of a multifaceted approach in histocompatibility testing. This is an attempt to compile the relevant information, providing a basis for comparison in a clear and foundational format for histocompatibility testing laboratories. Full article

There is no practical and at the same time objective colour system available for describing cultivated roses (Rosa L. cultivars). For this reason, a new colour classification system was developed which is colorimetrically balanced and appropriate for algorithmic colour identification; however, it is also suitable for field-work. The system is based on the following colorimetric criteria: (A) Each colour category is characterised by a measured petal colour in the CIE L*a*b* standard as the centroid of the category. (B) The CIEDE2000 colour differences between the adjacent centroid colours are limited (5 < ΔE₀₀ < 7). (C) The maximal colour difference between the measured colours in a category is also limited (to 12.12 ΔE₀₀). (D) A measured petal colour can only be classified into an existing category if the colour difference from the centroid colour of the given category is less than 5.81 ΔE₀₀, otherwise a new category is required. (E) A category is only considered non-redundant if it has at least one measured petal colour that cannot be classified elsewhere. (F) The classification of the petal colours is based on the least colour difference from the centroid colours. As a result, 133 colour categories were required for describing all the 8139 petal colours of the rose cultivars of the Budatétény Rose Garden (Hungary). Each colour category has the following parameters: standardised colour name, the colorimetric parameters of the centroid, grouping, RHS colour chart coding, and reference cultivars, which are described in the article. Full article

Potamanthidae belongs to the superfamily Ephemeroidea but has no complete mt genome released in the NCBI (except for two unchecked and one partial mt genome). Since the sister clade to Potamanthidae has always been controversial, we sequenced seven mt genomes of Potamanthidae (two species from Rhoenanthus and five species from Potamanthus) in order to rebuild the phylogenetic relationships of Potamanthidae in this study. The divergence time of Potamanthidae was also investigated by utilizing five fossil calibration points because of the indeterminate origin time. In addition, because Rhoenanthus coreanus and Potamanthus luteus are always in low-temperature environments, we aimed to explore whether these two species were under positive selection at the mt genome level. Amongst the 13 PCGs, CGA was used as the start codon in COX1, whereas other genes conformed to initiating with an ATN start codon. From this analysis, UUA (L), AUU (I), and UUU (F) had the highest usage. Furthermore, the DHU arm was absent in the secondary structure of S1 in all species. By combining the 13 PCGs and 2 rRNAs, we reconstructed the phylogenetic relationship of Potamanthidae within Ephemeroptera. The monophyly of Potamanthidae and the monophyly of Rhoenanthus and Potamanthus were supported in the results. The phylogenetic relationship of Potamanthidae + (Ephemeridae + Polymitarcyidae) was also recovered with a high prior probability. The divergence times of Potamanthidae were traced to be 90.44 Mya (95% HPD, 62.80–121.74 Mya), and the divergence times of Rhoenanthus and Potamanthus originated at approximately 64.77 Mya (95% HPD, 43.82–88.68 Mya), thus belonging to the late Pliocene Epoch or early Miocene Epoch. In addition, the data indicated that R. coreanus was under negative selection and that ATP8 and ND2 in Potamanthidae had a high evolutionary rate. Full article

Background: Chronic constipation, a prevalent gastrointestinal complaint, exhibits rising incidence and diverse clinical implications, especially among the aging population. This study aims to assess colonoscopy performance in chronic constipation across age groups, comprehensively evaluating diagnostic yield and comparing results with average-risk controls. Methods: A retrospective analysis was conducted on 50,578 colonoscopy procedures performed over 12 years, including 5478 constipated patients. An average-risk control group (n = 4100) was included. Data extracted from electronic medical records covered demographics, operational aspects, and colonoscopy findings. The primary outcome measures included the diagnosis rate of colorectal cancer (CRC), polyp detection rate (PDR), and inflammatory bowel disease (IBD) diagnoses in constipated patients versus controls, with age-based and multivariate analyses. Results: Constipated patients exhibiting lower rates of adequate bowel preparation (92.7% vs. 85.3%; p < 0.001) and a lower cecal intubation rate. No significant variances between CRC and PDR were observed between constipated and controls, except for a potential of a slightly elevated CRC risk in constipated patients older than 80 (2.50% vs. 0% in controls; p = 0.07). Multivariate analysis demonstrated, across all age groups, that constipation did not confer an increased risk for CRC or polyp detection. Younger constipated patients exhibited a higher rate of IBD diagnoses (1.7% vs. 0.1% in controls; p < 0.001). Conclusions: Constipation did not confer an increased risk for CRC or polyps, among any age groups, except for a potential signal of elevated CRC risk in patients older than 80; additionally, it was associated with higher rates of IBD in younger patients. Full article

Adequate experimental animal models play an important role in an objective assessment of the effectiveness of medicines and functional foods enriched with biologically active substances. The aim of our study was a comparative assessment of the effect of consumption of 1 or 2% cholesterol with and without regular (two times a week), moderate running exercise on the main biomarkers of lipid and cholesterol metabolism, as well as the intestinal microbiota of male Wistar rats. In experimental rats, a response of 39 indicators (body weight, food consumption, serum biomarkers, liver composition, and changes in intestinal microbiota) was revealed. Total serum cholesterol level increased 1.8 times in animals consuming cholesterol with a simultaneous increase in low-density lipoprotein cholesterol (2 times) and decrease in high-density lipoprotein cholesterol (1.3 times) levels compared to the control animals. These animals had 1.3 times increased liver weight, almost 5 times increased triglycerides level, and more than 6 times increased cholesterol content. There was a tendency towards a decrease in triglycerides levels against the background of running exercise. The consumption of cholesterol led to a predominance of the Bacteroides family, due to a decrease in F. prausnitzii (1.2 times) and bifidobacteria (1.3 times), as well as an increase in Escherichia family (1.2 times). The running exercise did not lead to the complete normalization of microbiota. Full article

The Galilee region of Israel boasts a rich ethnic diversity within its Arab population, encompassing distinct Muslim, Christian, Druze, and Bedouin communities. This preliminary exploratory study uniquely examined potential ethnic and gender differences in ischemic stroke characteristics across these Arab subgroups, which are seldom investigated separately in Israel and are typically studied as a homogeneous “Arab” sector, despite significant variations in their ethnicity, culture, customs, and genetics. The current study aimed to comparatively evaluate stroke characteristics, including recurrence rates, severity, and subtypes, within and across these distinct ethnic groups and between genders. When examining the differences in stroke characteristics between ethnic groups, notable findings emerged. The Bedouin population exhibited significantly higher rates of recurrent strokes than Muslims (M = 0.55, SD = 0.85 vs. M = 0.25, SD = 0.56; p < 0.05). Large vessel strokes were significantly more prevalent among Christians (30%) than Druze (9.9%; p < 0.05). Regarding gender differences within each ethnic group, several disparities were observed. Druze women were six times more likely to experience moderate to severe strokes than their male counterparts (p < 0.05). Interestingly, Druze women also exhibited a higher representation of cardio-embolic stroke (19.8%) compared with Druze men (4.6%; p < 0.001). These findings on the heterogeneity in stroke characteristics across Arab ethnic subgroups and by gender underscore the need to reconsider the approach that views all ethnic groups comprising the Arab sector in Israel as a homogeneous population; instead, they should be investigated as distinct communities with unique stroke profiles, requiring tailored culturally aware community-based prevention programs and personalized therapeutic models. The identified patterns may guide future research to develop refined, individualized, and preventive treatment approaches targeting the distinct risk factors, healthcare contexts, and prevention needs of these diverse Arab populations. Full article

The olive fruit fly, known as Bactrocera oleae (Rossi) (Diptera: Tephritidae), is causing substantial economic losses in olive crops worldwide. Studying the activity patterns of the insect may expand our knowledge to eventually adopt more sustainable and effective pest control approaches. In the present study, we investigated the impact of food and water deprivation on the mobility of olive fruit flies using a modified version of the LAM25 system (locomotor activity monitor)—Trikinetics, an automated locomotor activity electronic device. Both male and female flies at four different age groups, reared on olives in the laboratory, were individually placed in glass tubes. Their locomotor activity was recorded every minute by three monitors within the digital device over a three-day period. Our observations revealed that adults exhibited significantly reduced movement during nighttime compared to daytime. The greatest mobility was observed during the period of 15:00 to 20:59. Additionally, younger flies demonstrated higher levels of mobility compared to older ones. Flies subjected to both food and water deprivation exhibited higher mobility compared to the control group. These insights offer valuable insights for enhancing pest management strategies aimed at controlling olive fruit flies adopting a more sustainable approach. Full article

Hemicellulose extracted by alkali treatment is of interest because of the advantages of its intact sugar structure and high degree of polymerization. However, the hemicellulose extracted by alkali treatment contained more lignin fragments and the presence of a lignin–carbohydrate complex (LCC), which affected the isolation and purification of hemicellulose and its comprehensive utilization. Therefore, the evaluation of the LCC structure of different types of lignocellulosic resources is of great significance. In this study, the LCC structures of hardwoods and Gramineae were enriched in alkaline systems. Information on the composition, structural proportions, and connection patterns of LCC samples was discussed. The similarities and differences between the LCC structures of different units of raw materials were comparatively studied. The results indicated that the monosaccharide fractions were higher in the LCC of Gramineae compared to hardwoods. The composition of the lignin fraction was dominated by G and S units. The phenyl glycosidic (PhGlc) bond is the predominant LCC linkage under alkali-stabilized conditions. In addition, Gramineae PhGlc types are more numerous compared to hardwoods. The results of the study provide insights into the differences in the chemical composition and structural features of LCC in different plants and provide important guidance for the optimization of the process of purifying hemicellulose. Full article

Cereal grains and pulses are staple foods worldwide, being the primary supply of energy, protein, and fiber in human diets. The current practice of milling and fractionation yields large quantities of byproducts and waste, which are largely downgraded and end up as animal feeds or fertilizers. This adversely affects food security and the environment, and definitely implies an urgent need for a sustainable grain processing system to rectify the current issues, particularly the management of waste and excessive use of water and energy. The current review intends to discuss the limitations and flaws of the existing practice of grain milling and fractionation, along with potential solutions to make it more sustainable, with an emphasis on wheat and peas as common fractionation crops. This review discusses a proposed sustainable grain processing system for the fractionation of wheat or peas into flour, protein, starch, and value-added components. The proposed system is a hybrid model that combines dry and wet fractionation processes in conjunction with the implementation of three principles, namely, integration, recycling, and upcycling, to improve component separation efficiency and value addition and minimize grain milling waste. The three principles are critical in making grain processing more efficient in terms of the management of waste and resources. Overall, this review provides potential solutions for how to make the grain processing system more sustainable. Full article

The medicinal plant tulsi (Ocimum sanctum L.) is acknowledged for its invigorating and healing properties that enhance resilience to stress in various human and animal models by modulating antioxidant compounds. While extensive research has documented these effects in humans, the adaptogenic potential of tulsi in stressful in vitro plant systems has not been explored. This study aimed to elucidate the adaptogenic properties of tulsi leaf extract on the in vitro regeneration of tobacco leaf explants through an investigation of the indoleamines at different developmental stages. Shoot regeneration from leaf explants on the medium supplemented with tulsi extract (20%) was compared to the control, and the differences in indoleamine compounds were analyzed using ultra-performance liquid chromatography. Treatment of the explants with the extract resulted in an almost two-fold increase in the number of regenerants after four weeks of culture, and 9% of the regenerants resembled somatic embryo-like structures. The occurrence of browning in the extract-treated explants stopped on day 10, shoots began to develop, and a significant concentration of tryptamine and N-acetyl-serotonin accumulated. A comparative analysis of indoleamine compounds in intact and cut tobacco leaves also revealed the pivotal role of melatonin and 2-hydroxymelatonin functioning as antioxidants during stress adaptation. This study demonstrates that tulsi is a potent adaptogen that is capable of modulating plant morphogenesis in vitro, paving the way for further investigations into the role of adaptogens in plant stress biology. Full article

Sepsis is a life-threatening condition that affects 1.2 million children annually. Although there are several criteria for diagnosing this condition, signs are often nonspecific, and identifying sepsis is challenging. In this context, presepsin (P-SEP) seems to be a promising new biomarker since its plasma levels increase earlier than other sepsis-related proteins and its measurement is faster. We enrolled 157 minors who presented to the Pediatric Emergency Department of Agostino Gemelli Hospital with fever and suspected sepsis. Biochemical, anamnestic, and clinical data were collected. Viral agents were identified as the causative factor in 64 patients, who had an average P-SEP value of 309.04 pg/mL (SD ± 273.2), versus an average P-SEP value of 526.09 pg/mL (SD ± 657) found in 27 bacterial cases (p value: 0.0398). Four cases of overt sepsis had an average P-SEP value of 3328.5 pg/mL (SD ± 1586.6). The difference in P-SEP levels in viral versus bacterial infections was found to be statistically significant; therefore, P-SEP may have a central role in the evaluation of febrile children, helping clinicians distinguish between these two etiologies. Furthermore, amongst the cases of confirmed sepsis, P-SEP was always greater than 2000 pg/mL, while C-reactive protein and procalcitonin values appeared lower than what was considered significant. Full article

Patients with predominantly antibody deficiencies (PADs) display hypogammaglobulinemia with a high prevalence of infections, along with autoimmune manifestations, benign and malignant lymphoproliferation and granulomatous disease. It is noteworthy that PAD patients, even those with defects in the same causative genes, display a variable clinical phenotype, suggesting that additional genetic polymorphisms, located in either immune-related or non-immune-related genes, may affect their clinical and laboratory phenotype. In this context, we analyzed 80 PAD patients, including 70 with common variable immunodeficiency (CVID) for SERPINA1 defects, in order to investigate the possible contribution to PAD clinical phenotype. Ten CVID patients carried heterozygous pathogenic SERPINA1 defects with normal alpha-1 antitrypsin levels. Interestingly, the presence of the Z allele (rs28929474), which was found in three patients, was significantly associated with liver disease; hepatic complications were also observed in patients carrying the p.Leu23Gln (rs1379209512) and the p.Phe76del (rs775982338) alleles. Conversely, no correlation of SERPINA1 defective variants with respiratory complications was observed, although patients with pathogenic variants exhibit a reduced probability of developing autoimmune diseases. Therefore, we recommend SERPINA1 genetic analysis in PAD in order to identify patients with a higher risk for liver disease. Full article

Although cardiac resynchronization therapy (CRT) reduces morbidity and mortality and reverses left ventricular (LV) remodeling in heart failure patients with LV electrical dyssynchrony, induced proarrhythmia has been reported. The mechanism of CRT-induced proarrhythmia remains under debate. In this case report, a description of how LV pacing induced polymorphic ventricular tachycardia immediately after the initiation of CRT has been reported. By changing the pacing configuration using a multipoint pacing stimulation, we can assume that induced ventricular tachycardia is related to the reentry mechanism facilitated by the unidirectional block. As a result, a multipoint pacing (MPP) configuration near the scar area can avoid the onset of a unidirectional block with the establishment of the reentry phenomenon, thus avoiding induced VTs. Full article

The high dimensionality of hyperspectral images (HSIs) brings significant redundancy to data processing. Band selection (BS) is one of the most commonly used dimensionality reduction (DR) techniques, which eliminates redundant information between bands while retaining a subset of bands with a high information content and low noise. The wild horse optimizer (WHO) is a novel metaheuristic algorithm widely used for its efficient search performance, yet it tends to become trapped in local optima during later iterations. To address these issues, an enhanced wild horse optimizer (IBSWHO) is proposed for HSI band selection in this paper. IBSWHO utilizes Sobol sequences to initialize the population, thereby increasing population diversity. It incorporates Cauchy mutation to perturb the population with a certain probability, enhancing the global search capability and avoiding local optima. Additionally, dynamic random search techniques are introduced to improve the algorithm search efficiency and expand the search space. The convergence of IBSWHO is verified on commonly used nonlinear test functions and compared with state-of-the-art optimization algorithms. Finally, experiments on three classic HSI datasets are conducted for HSI classification. The experimental results demonstrate that the band subset selected by IBSWHO achieves the best classification accuracy compared to conventional and state-of-the-art band selection methods, confirming the superiority of the proposed BS method. Full article

The screening of all non-radioactive metals without lanthanides for thermochemical hydrogen storage was performed based on physical chemistry calculations. The thermodynamic data were collected from the NIST (National Institute of Standards and Technology) public data repository, which was followed by calculations regarding the change in enthalpy, entropy, Gibbs free energy and equilibrium reaction temperature. The results were critically evaluated based on thermodynamic parameters, viable metals were identified, and their hydrogen storage densities and energy–enthalpy ratios were evaluated. The elements viable for controlled thermochemical hydrogen storage via the reversible reduction and oxidation of metal oxides and metals are manganese (Mn), iron (Fe), molybdenum (Mo) and tungsten (W). Manganese has the largest theoretical potential for hydrogen storage with reversible reduction and oxidation of metal oxides and metals. The second candidate is iron, while the other two (Mo and W) have much lower potential. More research efforts should be dedicated to experimental testing of the identified metals (Mn, Fe, Mo and W) and their different oxides for thermochemical hydrogen storage capabilities both on laboratory and pilot scales. Ferromanganese alloy(s) might also prove itself as an efficient and affordable thermochemical hydrogen storage material. Our theoretical investigation expanded the knowledge on thermochemical hydrogen storage and is accompanied with a brief literature review revealing the lack of experimental studies, especially on oxidation of metals with water vapor occurring during the hydrogen release phase of the cycle. Consequently, accurate modelling of transport, kinetics and other phenomena during hydrogen storage and release is scarce. Full article

A total of 281 coleopteran species from 41 families were recorded from different sites of an abandoned cut-over peatland designated as the Carbon Measurement Supersite in Kaliningrad Oblast. This beetle assemblage is considered a baseline (pre-impact) faunal assemblage for further investigations during the ‘before–after’ (BA) or ‘before–after control-impact’ (BACI) study on a peatland that is planned to be rewetted. The spontaneously revegetated peatland has a less specialised beetle assemblage than at an intact raised bog. Tyrphobiontic species are completely absent from the peatland, while some tyrphophiles (5.3% of the total beetle fauna) are still found as remnants of the former raised bog communities. The predominant coenotic coleopteran group is tyrphoneutral generalists from various non-bog habitats (72.9%). The species composition is associated with the vegetation structure of the disturbed peatland (fragmentary Sphagnum cover, lack of open habitats, and widespread birch coppice or tree stand), which does not correspond to that of a typical European raised bog. The sampled coleopteran assemblage is divided into several relative ecological groups, whose composition and peculiarities are discussed separately. Possible responses to the rewetting measurements in different coleopteran groups are predicted and briefly discussed. A complex assemblage of stenotopic peatland-specialised tyrphophiles (15 spp.) and the most abundant tyrphoneutral generalists (31 spp.) were assigned as indicators for the environmental monitoring of peatland development. Full article

To reveal the mechanism of hydraulic fracture initiation and propagation under the conditions of multiple perforations during horizontal well fracturing, we creatively conducted dual-hole fracturing experiments on small rock samples and established a two-dimensional model of a single cluster with multiple perforations in a horizontal well based on the extended finite element method using the fluid–solid coupling equation, which was combined with the basic theory of damage mechanics. The biggest difference from previous research is that this model does not consider the hypothesis of stress shadows and only focuses on studying the initiation and propagation of multiple perforations in one cluster. We studied the effects of perforation parameters, stress state, and injection flow rate on the initiation and propagation of hydraulic fractures using this model. The experimental and simulation results indicate that under multi-perforation conditions, the number of fractures depends on the number of perforations. The simulation results show that when the spacing between perforations increases or the number of perforations reduces, the initiation time of perforation is advanced and the interference between fractures weakens, which is conducive to the initiation and propagation of hydraulic fractures. As the stress difference increases, the initiation time of perforation becomes earlier and the deflection angle of the outermost fractures becomes smaller, which is conducive to the parallel expansion of the fractures. Moreover, although this has little impact on the morphology of fractures with the rise in flow rate in simulation, it is beneficial for improving the initiation and propagation speed of fractures. The length of fractures also increases significantly at the same time point. In addition, both the experiments and simulations revealed that an increase in the flow rate could accelerate the initiation time of fractures. The proposed model can guide fracturing construction to optimize the design of perforation spacing during horizontal well fracturing, which can contribute to reducing development costs and improving the final production. Full article

The distribution of liverworts in the North Pacific is still poorly understood. Scapania umbrosa, which is rare in Asia, was first recorded during the study of the liverwort flora of the Commander Islands (the westernmost Aleutians). The results of the molecular genetic analysis not only confirmed that the specimen belonged to Scapania umbrosa but also demonstrated the genetic specificity of the discovered population in comparison with the European, Siberian, and Alaskan populations. The island population of Scapania umbrosa is likely a recently diverged isolate of the species. Previously reported data on the distribution of Scapania umbrosa in the Russian Far East from Kamchatka and Kunashir (VBGI Herbarium), as well as from Sakhalin and Southern Primorye (reported in GBIF), are based on misidentifications. Morphological descriptions, distinguishing characters, line drawings, and photographs of Scapania umbrosa based on the collected material are provided. Full article

In the process of the large-scale hydraulic fracturing of a shale gas field in the Weiyuan area of Sichuan province, the quantitative description and evaluation of hydraulic fracture expansion morphology and the three-dimensional distribution law are the key points of evaluation of block fracturing transformation effect. Many scholars have used the finite element method, discrete element method, grid-free method and other numerical simulation methods to quantitatively characterize hydraulic fractures, but there are often the problems that the indoor physical simulation results are much different from the actual results and the accuracy of most quantitative studies is poor. Considering rock mechanics parameters and based on the displacement discontinuity method (DDM), a single-stage multi-cluster fracture propagation model of horizontal well was established. The effects of Young’s modulus, Poisson’s ratio, the in situ stress difference, the approximation angle, the perforation cluster number and the perforation spacing on the formation of complex fracture networks and on the geometrical parameters of hydraulic fractures were simulated. The research results can provide theoretical reference and practical guidance for the optimization of large-scale fracturing parameters and the quantitative post-fracturing evaluation of horizontal wells in unconventional reservoirs such as shale gas reservoirs. Full article

Epidemiologic studies have established that mpox (formerly known as monkeypox) outbreaks worldwide in 2022–2023, due to Clade IIb mpox virus (MPXV), disproportionately affected gay, bisexual, and other men who have sex with men. More than 35% and 40% of the mpox cases suffer from co-infection with HIV and sexually transmitted infections (STIs) (e.g., Chlamydia trachomatis, Neisseria gonorrhoeae, Treponema pallidum, and herpes simplex virus), respectively. Bacterial superinfection can also occur. Co-infection of MPXV and other infectious agents may enhance disease severity, deteriorate outcomes, elongate the recovery process, and potentially contribute to the morbidity and mortality of the ensuing diseases. However, the interplays between MPXV and HIV, bacteria, other STI pathogens and host cells are poorly studied. There are many open questions regarding the impact of co-infections with HIV, STIs, or bacterial superinfections on the diagnosis and treatment of MPXV infections, including clinical and laboratory-confirmed mpox diagnosis, suboptimal treatment effectiveness, and induction of antiviral drug resistance. In this review article, we will discuss the progress and knowledge gaps in MPXV biology, antiviral therapy, pathogenesis of human MPXV and its co-infection with HIV, STIs, or bacterial superinfections, and the impact of the co-infections on the diagnosis and treatment of mpox disease. This review not only sheds light on the MPXV infection and co-infection of other etiologies but also calls for more research on MPXV life cycles and the molecular mechanisms of pathogenesis of co-infection of MPXV and other infectious agents, as well as research and development of a novel multiplex molecular testing panel for the detection of MPXV and other STI co-infections. Full article

Depression is associated with pathological changes and metabolic abnormalities in multiple brain regions. The simultaneous comprehensive and in situ detection of endogenous molecules in all brain regions is essential for a comprehensive understanding of depression pathology, which is described in this paper. A method based on desorption electrospray ionization mass spectrometry imaging (DESI-MSI) technology was developed to classify mouse brain regions using characteristic lipid molecules and to detect the metabolites in mouse brain tissue samples simultaneously. The results showed that characteristic lipid molecules can be used to clearly distinguish each subdivision of the mouse brain, and the accuracy of this method is higher than that of the conventional staining method. The cerebellar cortex, medial prefrontal cortex, hippocampus, striatum, nucleus accumbens-core, and nucleus accumbens-shell exhibited the most significant differences in the chronic social defeat stress model. An analysis of metabolic pathways revealed that 13 kinds of molecules related to energy metabolism and purine metabolism exhibited significant changes. A DESI-MSI method was developed for the detection of pathological brain sections. We found, for the first time, that there are characteristic changes in the energy metabolism in the cortex and purine metabolism in the striatum, which is highly important for obtaining a deeper and more comprehensive understanding of the pathology of depression and discovering regulatory targets. Full article