Abstract
Two difficulties are frequently encountered when it is desired to grow pathogenic bacteria with active aeration, namely, foaming and wetting of the exhaust filter. Chemical or mechanical foam-breaking and heating systems can be used to avoid these problems but have many disadvantages. I have used them mainly on large-scale equipment1,2. However, it was found that a basically different approach had to be used for ordinary laboratory equipment. Since the method which has been developed may have wide applications, it will be described below.
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References
Hedén, C.-G., Malmgren, B., Sundstrom, K. E., and Tornqvist, B., Acta Path. Microbiol. Scand., 30, 284 (1952).
Hedén, C.-G., and Malmgren, B., Indust. Eng. Chem., 46, 1747 (1954).
Cooper, C. M., Fernstrom, G. A., and Miller, S. A., Indust. Eng. Chem., 36, 504 (1944).
Chain, E. B., Paladino, S., Callow, D. S., Ugolini, F., and Van der Sluis, J., Bull. World Health Org., 6, 73 (1952).
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HEDÉN, CG. Pulsating Aeration of Microbial Cultures. Nature 179, 324–325 (1957). https://doi.org/10.1038/179324b0
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DOI: https://doi.org/10.1038/179324b0
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