Abstract
THE paper chromatography of deoxyribonucleosides has been described by several workers1–5. The compounds can be located on the chromatogram by examination under ultra-violet light1,6,7, by a microbiological method2,3,5 or by means of the Feulgen or Dische reactions4. A colour reaction between deoxyribonucleic acid and cysteine in sulphuric acid has been reported by Dische8,9. This has now been applied to the detection of deoxyribonucleosides on paper chromatograms. While all the deoxyribosides, as well as deoxyribose itself, give the reaction, neither ribose nor the ribonucleosides can be detected by this method.
Similar content being viewed by others
Article PDF
References
Hotchkiss, J. Biol. Chem., 175, 315 (1948).
Cuthbertson and Lester Smith, Biochem. J., 44, v (1949); 45, xii (1949).
Winsten and Eigen, J. Biol. Chem., 177, 989 (1949); 181, 109 (1949).
Buchanan, Dekker and Long, J. Chem. Soc., 3162 (1950).
Shive, Sibley and Rogers, J. Amer. Chem. Soc., 73, 867 (1951).
Markham and Smith, Nature, 163, 250 (1949); Biochem. J., 45, 294 (1949).
Holiday and Johnson, Nature, 163, 216 (1949).
Dische, Proc. Soc. Exp. Biol. Med., 55, 217 (1944).
Stumpf, J. Biol. Chem., 169, 367 (1947).
Carter, J. Amer. Chem. Soc., 72, 1466 (1950).
Dekker and Elmore, J. Chem. Soc., 2864 (1951).
Hanes and Isherwood, Nature, 164, 1107 (1949).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
BUCHANAN, J. Detection of Deoxyribonucleosides on Paper Chromatograms. Nature 168, 1091 (1951). https://doi.org/10.1038/1681091a0
Issue Date:
DOI: https://doi.org/10.1038/1681091a0
This article is cited by
-
Is the cytoplasm a site of nuclear DNA synthesis?
Nature (1974)
-
Antibodies recognise specific structures of triple-helical polynucleotides built on poly(A) or poly(dA)
Nature (1974)
-
Use of Acidic Degradation for the Determination of the Distribution of Pyrimidine Nucleotides in Deoxyribonucleic Acids
Nature (1966)
-
Enzymatic synthesis of 5-bromo-2’-deoxyuridine-2-14C and of 5-iodo-2’-deoxyuridine-2-14C and their incorporation into deoxyribonucleic acid (Allium cepa)
Biologia Plantarum (1964)
-
Deoxycytidine in Urine as an Indicator of Changes after Irradiation
Nature (1958)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.