Summary
The structure of synchronized cells of Chlorella pyrenoidosa was investigated using the freeze-etching method.
The report presents some various cell stages showing especially successive aspects of chloroplasts, of the vacuolar system and of some membranous and lamellar structures (nuclear, partition and thylakoid membranes) during the life cycle of the algae.
Lamellar surfaces of chloroplasts are visible. These surfaces are beset with two classes of particles, which have an average diameter of 120 Å and 60 Å; the 120 Å particle are generally composed of 3 to 4 subunits having a diameter of 60 Å.
These particles were counted on the thylakoid surfaces, where they are the most numerous.
During the light period of the synchronous culture, the total number of particles/μ2 on lamellar surfaces remains almost constant: an average of 1500 to 2500 units/μ2 was observed.
During the dark period, on the other hand, a significative increase of the number of particle/μ2 occurs in mother cells 2 hrs. before autospore separation. The average values range from 2000 to 4000 units/μ2.
It appears that the highest concentrations of 120 and 60 Å particles on the thylakoid surfaces are found in cells in which the photosynthetic O2 evolution per unit of chlorophyll is minimum (according to the publications of other authors). High concentrations of particles seem to characterize the newly synthesized parts of the thylakoids.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
Bibliographie
Bisalputra, T., F. M. Ashton, and T. E. Weier: Role of dictyosomes in wall formation during cell division of Chlorella vulgaris. Amer. J. Bot. 53, 213–216 (1966).
Bourdu, R., et M. Lefort: Structure fine, observée en cryodécapage, des lamelles photosynthétiques de Cyanophycées endosymbiotiques: Glaucocystis nostochinearum Itzigs et Cyanophora paradoxa Korschikoff. C. R. Acad. Sci. (Paris) 265, 37–40 (1967).
Branton, D., and R. B. Park: Subunits in chloroplast lamellae. J. Ultrastruct. Res. 19, 283–303 (1967).
Gehardt, B.: Untersuchungen über Beziehungen zwischen Ascorbinsäure und Photosynthese. Planta (Berl.) 61, 101–129 (1964).
Gilet, R., G. Giraud et N. Hauswirth: Variations cytologiques subies par Chlorella pyrenoidosa au cours d'un cycle dans une culture synchrone. J. Micros. 6, 56a (1967).
Howell, S. H., and E. N. Moudrianakis: (a) Hill reaction site in chloroplast membranes: non-participation of the quantasome particle in photoreduction. J. molec. Biol. 27, 323–333 (1967).
——: (b) Function of the “quantasome” in photosynthesis: Structure and properties of membrane-bound particle active in the dark reactions of photophosphorylation. Proc. nat. Acad. Sci. (Wash.) 58, 1261–1268 (1967).
Lefort, M., et R. Bourdu: Analyse en cryodécapage des infrastructures cellulaires de Chlorella pyrenoidosa. J. Micros. 5, 60a (1966).
Lorenzen, H.: Synchronization of Chlorella with light-dark changes and periodical dilution of the population to a standard cell number. In: Synchrony in cell division and growth (E. Zeuthen, éd.), p. 571–578. New York-London-Sydney: Interscience Publ. 1964.
Moor, H.: Die Gefrier-Fixation lebender Zellen und ihre Anwendung in der Elektronenmikroskopie. Z. Zellforsch. 62, 546–580 (1964).
—, and K. Mühlethaler: Fine structure in frozen etched yeast cells. J. Cell Biol. 17, 609–628 (1963).
Mühlethaler, K.: The ultrastructure of the plastid lamellae. In: Biochemistry of chloroplasts, vol. I, p. 49–64 (T. W. Goodwin, éd.). London and New York: Academic Press 1966.
—, H. Moor, and S. W. Szarkowski: The ultrastructure of the chloroplast lamellae. Planta (Berl.) 67, 305–323 (1965).
Murakami, S., Y. Morimura, and A. Takamiya: Electron microscopic studies along cellular life cycle of Chlorella ellipsoidea. In: Microalgae and photosynthetic bacteria, p. 65–83. Tokyo: University Tokyo Press (1963).
Park, R. B.: Substructure of chloroplast lamellae. J. Cell Biol. 27, 151–161 (1965).
—, et J. Biggins: Quantasome: size and composition. Science 144, 1009–1010 (1964).
—, et S. Drury: The effects of daylength on quantasome structure and chloroplast photochemistry in Spinacia oleracea L. var. Early hybrid 7. In: Le Chloroplaste (C. Sironval), p. 328–334. Paris: Masson & Cie. 1967.
Pirson, A., et H. Lorenzen: Synchronized dividing algae. Ann. Rev. Plant Physiol. 17, 439–458 (1966).
Ruppel, H. G.: Untersuchungen über die zusammensetzung von Chlorella bei Synchronisation im Licht-Dunkel-Wechsel. Flora (Jena) 152, 113–138 (1962).
Senger, H., and N. I. Bishop: Quantum yield of photosynthesis in synchronous Scenedesmus cultures. Nature (Lond.) 214, 140–142 (1967).
Soeder, C. J.: Elektronenmikroskopische Untersuchung der Protoplastenteilung bei Chlorella fusca Shihira et Krauss. Arch. Mikrobiol. 50, 368–377 (1965).
Staehelin, A.: Die Ultrastruktur der Zellwand und des Chloroplasten von Chlorella. Z. Zellforsch. 74, 325–350 (1966).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Guérin-Dumartrait, E. Étude, en cryodécapage, de la morphologie des surfaces lamellaires chloroplastiques de Chlorella pyrenoidosa, en cultures synchrones. Planta 80, 96–109 (1968). https://doi.org/10.1007/BF00387194
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00387194