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Identification and characterization of suppressor of cytokine signaling 1 (SOCS-1) homologues in teleost fish

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Abstract

The suppressor of cytokine signaling 1 (SOCS-1), as the name implies, is a member of proteins that regulate cytokine signaling pathways by inhibiting the events of key tyrosine phosphorylation on cytokine receptors and signaling molecules such as Janus kinase family members. Although mammalian SOCS-1 homologues were characterized in several species, no similar research work has been reported in fish yet. In this paper, we initially cloned the SOCS-1 genes from Tetraodon nigroviridis and Danio rerio, and identified other two SOCS-1 genes from Fugu rubripes and Gasterosteus aculeatus. The results showed that the fish SOCS-1-encoding genes consist of two exons and a single intron, a typical characteristic of SOCS family in gene organization. Moreover, two alternatively spliced transcripts that encoded 220 and 196 amino acids were obtained in T. nigroviridis, proving the distinct existence of alternative splicing in fish SOCS-1 different from higher vertebrates. By reverse transcriptase polymerase chain reaction (PCR) and real-time quantitative PCR, gene expression studies indicated that both two alternatively spliced transcripts of Tetraodon SOCS-1 were expressed extensively in major tissues as we examined and their corresponding expression levels could be strikingly raised at 3 h postinjection with lipopolysaccharide, which strongly suggested that SOCS-1 proteins in fish might be involved in inflammatory responses. This is the first report of cloning and characterization of SOCS-1 complementary deoxyribonucleic acids and genes in fish.

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Acknowledgment

This work was supported by grants from the National Basic Research Program of China (973) (2006CB10805), the National Natural Science Foundation of China (30371096, 30571423), Zhejiang Natural Science Foundation (Z305039), the Science and Technology Program of Zhejiang (2006C12038, 2006C23045, 2005E10001), and Specialized Research Fund for the Doctoral Program of Higher Education (20030335040).

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  1. College of Life Sciences, Zhejiang University, Hangzhou, 310058, China

    Hong-Jian Jin, Li-Xin Xiang & Jian-Zhong Shao

  2. College of Life Sciences, China Jiliang University, Hangzhou, 310018, China

    Hong-Jian Jin

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  1. Hong-Jian Jin
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Jin, HJ., Xiang, LX. & Shao, JZ. Identification and characterization of suppressor of cytokine signaling 1 (SOCS-1) homologues in teleost fish. Immunogenetics 59, 673–686 (2007). https://doi.org/10.1007/s00251-007-0232-8

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