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Influence of beta-aminoproprionitrile (BAPN) on cell growth and elastic fiber formation in cultures of auricular chondrocytes

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Summary

Auricular chondrocytes isolated from 4-day-old rabbits and grown in vitro for 14 days, proliferated rapidly and produced a conspicuous network of elastic fibers. Beta-aminoproprionitrile (BAPN), which in vivo inhibits cross-linking of elastin, decreased the formation of elastic fibers at a concentration of 10–20 μg/ml and prevented formation at 40 μg/ml. At a concentration of 5 μg/ml only the so-called patches of elastin appeared to be absent. The inhibitory effect of BAPN on cell growth did not exceed 10%, which indicates that BAPN is only slightly harmful to auricular chondrocytes and can safely be used in studies on elastin deposition by these cells in vitro.

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Author notes

  1. S. Moskalewski

    Present address: Department of Histology and Embryology, School of Medicine, Chalubinskiego 5, PL-02-004, Warsaw, Poland

Authors and Affiliations

  1. Laboratory of Cell Biology and Histology, University of Leiden, Rijnsburgerweg 10, NL-2333 AA, Leiden, The Netherlands

    S. Moskalewski, C. H. Langeveld & J. P. Scherft

Authors
  1. S. Moskalewski
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  2. C. H. Langeveld
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  3. J. P. Scherft
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Moskalewski, S., Langeveld, C.H. & Scherft, J.P. Influence of beta-aminoproprionitrile (BAPN) on cell growth and elastic fiber formation in cultures of auricular chondrocytes. Experientia 39, 1147–1148 (1983). https://doi.org/10.1007/BF01943153

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  • DOI: https://doi.org/10.1007/BF01943153

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