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A candidate of organum vasculosum of the lamina terminalis with neuronal connections to neurosecretory preoptic nucleus in eels

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Abstract

Systemic angiotensin II (Ang II) is a dipsogen in terrestrial vertebrates and seawater teleosts. In eels, Ang II acts on the area postrema, a sensory circumventricular organ (CVO) and elicits water intake but other sensory CVOs have not yet been found in the eel forebrain. To identify sensory CVOs in the forebrain, eels were peripherally injected with Evans blue, which immediately binds to albumin, or a rabbit IgG protein. Extravasation of these proteins, which cannot cross the blood–brain barrier (BBB), was observed in the brain parenchyma of the anteroventral preoptic recess (PR) walls. Fenestrated capillaries were observed in the parenchymal margin of the ventral wall of the PR, confirming a deficit of the BBB in the eel forebrain. Immunostaining for tyrosine hydroxylase (TH) and choline acetyltransferase (ChAT) detected neurons in the lateral region of the anterior parvocellular preoptic nucleus (PPa), which were strongly stained by BBB-impermeable N-hydroxysulfosuccinimide. In the periventricular region of the PPa, many neurons incorporated biotinylated dextran amine conjugated to fluorescein, a retrograde axonal tracer, injected into the magnocellular preoptic nucleus (PM), indicating neuronal connections from the PPa to the PM. The mammalian paraventricular and supraoptic nuclei, homologous to the teleost PM, receive principal neuronal projections from the organum vasculosum of the lamina terminalis (OVLT). These results strongly suggest that the periventricular subpopulation of the PPa, which is most likely to be a component of the OVLT, serves as a functional window of access for systemic signal molecules such as Ang II.

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Acknowledgments

The authors thank Prof. Y. Furukawa (Hiroshima University) and Dr. M. Ando (The University of Tokyo) for valuable suggestions. This work was supported in part by a Grant-in-Aid for Scientific Research (A) (no. 23247010 to Takei Y) from JSPS.

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Authors and Affiliations

  1. Laboratory of Integrative Physiology, Graduate School of Integrated Arts & Sciences, Hiroshima University, 1-7-1 Kagamiyama, Higashi-hiroshima, Hiroshima, 739-8521, Japan

    Takao Mukuda

  2. Laboratory of Neurobiology, Graduate School of Integrated Arts & Sciences, Hiroshima University, Hiroshima, Japan

    Sawako Hamasaki & Yuka Koyama

  3. Laboratory of Physiology, Atmosphere and Ocean Research Institute, The University of Tokyo, Chiba, Japan

    Yoshio Takei

  4. Division of Medical Morphology, Department of Anatomy, Tottori University Faculty of Medicine, Tottori, Japan

    Toshiyuki Kaidoh

  5. Division of Morphological Analysis, Department of Anatomy, Tottori University Faculty of Medicine, Tottori, Japan

    Takao Inoué

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  1. Takao Mukuda
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  2. Sawako Hamasaki
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  3. Yuka Koyama
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  4. Yoshio Takei
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Correspondence to Takao Mukuda.

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Mukuda, T., Hamasaki, S., Koyama, Y. et al. A candidate of organum vasculosum of the lamina terminalis with neuronal connections to neurosecretory preoptic nucleus in eels. Cell Tissue Res 353, 525–538 (2013). https://doi.org/10.1007/s00441-013-1663-1

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  • DOI: https://doi.org/10.1007/s00441-013-1663-1

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