Skip to main content
SpringerLink
Log in

Molecular identification and biodiversity of potential allergenic molds (Aspergillus and Penicilium) in the poultry house: first report

  • Brief communication
  • Published:
Aerobiologia Aims and scope Submit manuscript

Abstract

The production of poultry has proved to be a significant source of fungi. Penicillium and Aspergillus are among the most important species, because they can cause irritations, infections, allergies, spoilage of food and beverages and are able to produce dangerous mycotoxins. Detection and identification of the species in these genera is very significant, because it provides relevant information about the properties of the responsible strains. Therefore, their taxonomic was determined using a phenotypic and molecular (ITS and partial β-tubulin sequences) methods. Investigations were conducted in the poultry house located near Wrocław in Lower Silesia (Poland). Using morphological analyses, 215 fungal strains were identified. Among them, 56 belonged to the Penicilium and Aspergillus genera. The results obtained from sequence analysis corresponded well with the morphological identification. Classification at species level was possible in most cases using the sequence data. Most isolates belong to Penicilium genus, with the follow dominating species (P. chrysogenum, P. polonicum and P. olsonii).

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

References

  • Balajee, S. A., Borman, A. M., Brandt, M. E., Cano, J., Cuenca-Estrella, M., et al. (2008). Sequence-based identification of Aspergillus, Fusarium, and Mucorales species in the clinical mycology laboratory: Where are we and where we should we go from here. Journal of Clinical Microbiology, 47(4), 877–884.

    Article  Google Scholar 

  • Borneman, J., & Hartin, R. J. (2000). PCR primers that amplify fungal rRNA genes from environmental samples. Applied and Environmental Microbiology, 66, 4356–4360.

    Article  CAS  Google Scholar 

  • Cardoso, P., de Queiroz, M., Pereira, O., & de Araújo, E. (2007). Morphological and molecular differentiation of the proteinase producing fungi Penicilium expansum and Penicilium griseoroseum. Brazilian Journal of Microbiology, 38, 71–78.

    Article  Google Scholar 

  • Colombo, F., Vallone, L., Giaretti, M., & Dragoni, I. (2003). Identification of Penicilium aurantiogriseum species with method of polymerase chain reaction–restriction fragment length polymorphism. Food Control, 14, 137–140.

    Article  CAS  Google Scholar 

  • Fairs, A., Agbetile, J., Hargadon, B., Bourne, M., Monteiro, W. R., Brightling, C. E., et al. (2010). IgE sensitization to Aspergillus fumigatus is associated with reduced lung function in asthma. American Journal of Respiratory and Critical Care Medicine, 182, 1362–1368.

    Article  Google Scholar 

  • Gehlot, P., Purohit, D. K., & Singh, S. K. (2011). Molecular diagnostic of human pathogenic Aspergillus species. Indian Journal of Biotechnology, 10, 207–210.

    CAS  Google Scholar 

  • Gerrits, E., & Hoog, G. (1999). Variability and molecular diagnostics of the neurotropic species Cladophialophora bantiana. Studies in Mycology, 43, 151–162.

    Google Scholar 

  • Glass, N. L., & Donaldson, G. C. (1995). Development of primer sets designed for use with the PCR to amplify conserved genes from filamentous ascomycetes. Applied and Environmental Microbiology, 61(4), 1323–1330.

    CAS  Google Scholar 

  • Goodman, V., Müller, A., Gröger-Arndt, H., Schlosser, D., et al. (2011). Identification of spore specific allergens from Penicillium chrysogenum. Journal of Integrated OMICS, 1(2), 272–279.

    Google Scholar 

  • Hashimoto, K., Kawakami, Y., Asano, K., & Onji, Y. (2012). Mycotoxin production of some Aspergillus ochraceus and Aspergillus fumigatus isolated from the air. Mycotoxins, 62(1), 1–6.

    Article  CAS  Google Scholar 

  • Hawksworth, D. (1991). The fungal dimension of biodiversity: Magnitude, significance, and conservation. Mycological Research, 95, 641–655.

    Article  Google Scholar 

  • Hinrikson, H. P., Hurst, S. F., de Aguirre, L., & Morrison, C. J. (2005). Molecular methods for the identification of Aspergillus species. Medical Mycology, 43(1), 129–137.

    Article  Google Scholar 

  • Houbraken, J., Due, M., Varga, J., Meijer, M., Frisvad, J., & Samson, R. A. (2007). Polyphasic taxonomy of Aspergillus section Usti. Studies in Mycology, 59, 107–128.

    Article  CAS  Google Scholar 

  • Houbraken, J., Frisvad, J., & Samson, R. (2010). Taxonomy of Penicillium citrinum and related species. Fungal Diversity, 44, 117–133.

    Article  Google Scholar 

  • Houbraken, J., & Samson, R. (2011). Phylogeny of Penicillium and the segregation of Trichocomaceae into three families. Studies in Mycology, 70, 1–51.

    Article  CAS  Google Scholar 

  • Hung, W. T., Su, S. L., Siu, L. I., & Tsung, C. C. (2011). Rapid identification of allergenic and pathogenic molds in environmental air by an oligonucleotide array. BMC Infectious Diseases, 11, 91.

    Article  CAS  Google Scholar 

  • Junior, P. R. J., Yamamoto, A. C. A., Amadio, J. V. R., Martins, E. R., Leal, F. A., et al. (2012). Trichocomaceae: Biodiversity of Aspergillus spp and Penicillium spp residing in libraries. The Journal of Infection in Developing Countries, 6(10), 734–743.

    Google Scholar 

  • Knutsen, A. P., Bush, R. K., Demain, J. G., Denning, D. W., Dixit, A., et al. (2012). Fungi and allergic lower respiratory tract diseases. Journal of Allergy and Clinical Immunology, 129(2), 280–291.

    Article  Google Scholar 

  • Magnani, M., Fernandes, T., Prete, C. E. C., Homechim, M., Ono, E. Y. S., & Vilas-Boas, L. A. (2004). Identification of Aspergillus ssp. isolated from coffee beans. Scientia Agricola (Piracicaba, Braz.), 62(1), 45–49.

    Article  Google Scholar 

  • Matković, K., Vučemilo, M., Vinković, B., Pavičić, Ž., Matković, S., & Benić, M. (2009). Airborne fungi in a dairy barn with emphasis on microclimate and emission. Veterinarski Arhiv, 79(3), 207–218.

    Google Scholar 

  • Nichita, I., & Tirziu, E. (2008). Investigations on airborne fungi in poultry houses. Lucrări Stiintificate Medici Veterinară, XLI, 932–935.

    Google Scholar 

  • Park, H. S., Jung, K. S., Kim, S. O., & Kim, S. J. (1994). Hypersensitivity pneumonitis induced by Penicillium expansum in a home environment. Clinical and Experimental Allergy, 24(4), 383–385.

    Article  CAS  Google Scholar 

  • Samson, R. A., & Flannigan, B. (1994). Health implications of fungi in indoor environments. Air quality monographs. Amsterdam, The Netherlands: Elsevier.

    Google Scholar 

  • Samson, R. A., Seifert, K., Kuijpers, A., Houbraken, J., & Frisvad, J. C. (2004). Phylogenetic analysis of Penicilium subgenus Penicilium using partial B-tubuliny sequences. Studies in Mycology, 49, 175–200.

    Google Scholar 

  • Seifert, K., Samson, R., deWaard, R., Houbraken, J., Lesque, A., et al. (2007). Prospects for fungus identification using CO1 DNA barcodes, with Penicillium as a test case. Proceedings of the National Academy of Sciences, 104(10), 3901–3906.

    Article  CAS  Google Scholar 

  • Soliman, E. S., Sobeih, M. A. A., Ahmad, Z. H., Hussein, M. M., Abdel-Latiff, H., Moneim, A. A., et al. (2009). Seasonal epidemiological surveillance on bacterial and fungal pathogens in broiler farms in Egypt. International Journal of Poultry Science., 8(8), 720–727.

    Article  Google Scholar 

  • Tsui, C. K. M., Woodhall, J., Chen, W., Lévesque, C. A., Lau, A., Schoen, C. D., et al. (2011). Molecular techniques for pathogen identification and fungus detection in the environment. IMA Fungus, 2(2), 177–189.

    Article  Google Scholar 

  • Weber, S., Hofmann, A., Pilhofer, M., Wanner, G., et al. (2009). The diversity of fungi in aerobic sewage granules assessed by 18S rRNA gene and ITS sequence analyses. FEMS Microbiology Ecology, 68(2), 246–254.

    Article  CAS  Google Scholar 

Download references

Acknowledgments

We thank CBS-KNAW Fungal Biodiversity Centre, especially Prof. Dr. Dr. h.c. R. A. Samson-Head of Applied and Industrial Mycology for the possibility to work in his group and essential support. Martin Meijer is thanked for technical assistance.

Author information

Authors and Affiliations

  1. The Angelus Silesius State School of Higher Vocational Education, Wałbrzych, Poland

    Kinga Plewa-Tutaj & Elżbieta Lonc

  2. Department of Microbial Ecology and Environmental, Institute of Genetics and Microbiology, University of Wrocław, Wrocław, Poland

    Kinga Plewa-Tutaj & Elżbieta Lonc

Authors
  1. Kinga Plewa-Tutaj
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Elżbieta Lonc
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Kinga Plewa-Tutaj.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Plewa-Tutaj, K., Lonc, E. Molecular identification and biodiversity of potential allergenic molds (Aspergillus and Penicilium) in the poultry house: first report. Aerobiologia 30, 445–451 (2014). https://doi.org/10.1007/s10453-014-9339-1

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10453-014-9339-1

Keywords

Search

Navigation