Nisin is used as a natural food preservative because of its broad-spectrum antimicrobial activity against Gram-positive bacteria. However, free nisin is susceptible to various factors that reduce its antimicrobial activity. Milk protein, a protein derived from milk, has self-assembly properties and is a good carrier of bioactive substances. In this study, lactoferrin–nisin nanoparticles (L-N), bovine serum albumin–nisin nanoparticles (B-N), and casein–nisin nanoparticles (C-N) were successfully prepared by a self-assembly technique, and then their properties were investigated. The studies revealed that lactoferrin (LF) and nisin formed L-N mainly through hydrophobic interactions and hydrogen bonding, and L-N had the best performance. The small particle size (29.83 ± 2.42 nm), dense reticular structure, and good thermal stability, storage stability, and emulsification of L-N laid a certain foundation for its application in food. Further bacteriostatic studies showed that L-N enhanced the bacteriostatic activity of nisin, with prominent inhibitory properties against Listeria monocytogenes, Staphylococcus aureus, and Bacillus cereus, which mainly disrupted the cell membrane of the bacteria. The above results broaden our understanding of milk protein–nisin nanoparticles, while the excellent antibacterial activity of L-N makes it promising for application as a novel food preservative, which will help to improve the bioavailability of nisin in food systems. Full article

Glycogen synthase kinase-3β (GSK3β) not only plays a crucial role in regulating sperm maturation but also is pivotal in orchestrating the acrosome reaction. Here, we integrated single-molecule long-read and short-read sequencing to comprehensively examine GSK3β expression patterns in adult Diannan small-ear pig (DSE) testes. We identified the most important transcript ENSSSCT00000039364 of GSK3β, obtaining its full-length coding sequence (CDS) spanning 1263 bp. Gene structure analysis located GSK3β on pig chromosome 13 with 12 exons. Protein structure analysis reflected that GSK3β consisted of 420 amino acids containing PKc-like conserved domains. Phylogenetic analysis underscored the evolutionary conservation and homology of GSK3β across different mammalian species. The evaluation of the protein interaction network, KEGG, and GO pathways implied that GSK3β interacted with 50 proteins, predominantly involved in the Wnt signaling pathway, papillomavirus infection, hippo signaling pathway, hepatocellular carcinoma, gastric cancer, colorectal cancer, breast cancer, endometrial cancer, basal cell carcinoma, and Alzheimer’s disease. Functional annotation identified that GSK3β was involved in thirteen GOs, including six molecular functions and seven biological processes. ceRNA network analysis suggested that DSE GSK3β was regulated by 11 miRNA targets. Furthermore, qPCR expression analysis across 15 tissues highlighted that GSK3β was highly expressed in the testis. Subcellular localization analysis indicated that the majority of the GSK3β protein was located in the cytoplasm of ST (swine testis) cells, with a small amount detected in the nucleus. Overall, our findings shed new light on GSK3β’s role in DSE reproduction, providing a foundation for further functional studies of GSK3β function. Full article

High-risk termites in wood imported to the Republic of Korea are currently treated with methyl bromide (MB), which has ozone-depleting properties and is highly toxic. This study evaluated the effectiveness of sulfuryl fluoride (SF) as a quarantine treatment against Reticulitermes speratus Kolbe (Blattodea: Rhinotermitidae) in wood, along with its wood sorption and penetration capacity. The LCt₅₀ and LCt₉₉ values for SF were 30.87 and 42.53 mg h/L at 23 °C and 151.62 and 401.9 mg h/L at 5 °C, respectively. The SF Ct values did not significantly differ between dry and wet wood at loading ratios of 10%, 30%, and 50% at both 5 °C and 23 °C (p > 0.05). In a closed wooden cube, the LCt₅₀ and LCt₉₉ for SF for R. speratus were 31.59 and 53.34 mg h/L, respectively, indicating an excellent wood penetration ability. SF caused 100% termite mortality with a 90% loading ratio in the scale-up trials (500 L). The SF concentration during ventilation decreased below the threshold limit value (TLV) of 5 ppm within 30 min, confirming that the working conditions were safe. This study provides a basis for the use of SF as an alternative to MB for the treatment of termites in wood. Full article

(1) Background: Osteoarthritis (OA) is the most common joint disease in the world. It is chronic, systemic, progressive and disabling. Orthobiologics have the potential to positively alter the course of this disease. Therefore, the aim of this study is to evaluate the efficacy of SVF/ACP in the treatment of advanced osteoarthritis of the knee in an unfiltered patient population. We hypothesize that this therapy can improve the symptoms associated with osteoarthritis of the knee. We also hypothesize that there are patient-related factors that influence the efficacy of therapy. (2) Methods: Two hundred and thirteen patients with moderate to severe OA of the knee and SVF/ACP injection were recruited for this study. Patients were excluded if they did not provide informed consent or were not receiving SVF/ACP therapy. Pain, function, symptoms and quality of life were assessed using standardized scores (KOOS, WOMAC) before and after treatment. (3) Results: The VAS pain score was significantly reduced by at least 30% (p < 0.001). Knee function, as measured by the KOOS daily activity and sport scores, showed significant increases of 21% and 45%, respectively, at 6 months (p < 0.04). (4) Conclusions: Treatment of knee OA with SVF/ACP injection positively modifies the disease by significantly reducing pain and improving function. Full article

Serous effusion cytology is a pivotal diagnostic and staging tool in clinical pathology, valued for its simplicity and cost-effectiveness. Staining techniques such as Giemsa and Papanicolaou are foundational, yet the search for rapid and efficient alternatives continues. Our study assesses the efficacy of an in-house-developed BlueStain, a toluidine blue variant, within the International System for Reporting Serous Fluid Cytopathology (TIS), aiming to optimize diagnostic clarity and resource use. Materials and Methods: This section provides details on the cohort of 237 patients with serous effusions, the ethical approval process, sample collection, and staining procedures with BlueStain, Papanicolaou, and Giemsa. It also describes the microscopic evaluation criteria, scoring system, and statistical methods used to compare the stains. Results: BlueStain demonstrated notable performance, particularly in identifying malignant cells, presenting a competitive alternative to the Papanicolaou stain, which, despite higher quality indices in other categories, requires more resources and time. The study revealed that BlueStain might offer a valuable balance between quality and efficiency, especially in cases where rapid diagnostic turnaround is essential. Conclusions: Our findings suggest that BlueStain is a viable staining method in the context of serous effusions, capable of providing detailed cytomorphological analysis. While traditional stains hold their place for their established diagnostic clarity, BlueStain offers a rapid and resource-optimized alternative. The absence of definitive diagnostic criteria in the atypical category and the inherent sample heterogeneity underscores the necessity for adaptable staining methods like BlueStain. The study highlights the potential trade-offs between detail and practicality in staining techniques, advocating for further research into innovative methods that do not compromise diagnostic precision for cost and time efficiency. Full article

In this work, we report on the synthesis and investigation of new hybrid multifunctional iron oxide nanoparticles (IONPs) coated by coumarin-bound copolymer, which combine magneto- or photothermal heating with luminescent thermometry. A series of amphiphilic block copolymers, including Coum-C₁₁-PPhOx₂₇-PMOx₅₉ and Coum-C₁₁-PButOx₈-PMOx₄₂ bearing luminescent and photodimerizable coumarin moiety, as well as coumarin-free PPhOx₂₇-PMOx₅₇, were evaluated for their utility as luminescent thermometers and for encapsulating spherical 26 nm IONPs. The obtained IONP@Coum-C₁₁-PPhOx₂₇-PMOx₅₉ nano-objects are perfectly dispersible in water and able to provide macroscopic heating remotely triggered by an alternating current magnetic field (AMF) with a specific absorption rate (SAR) value of 240 W.g⁻¹ or laser irradiation with a photothermal conversion efficiency of η = 68%. On the other hand, they exhibit temperature-dependent emission of coumarin offering the function of luminescent thermometer, which operates in the visible region between 20 °C and 60 °C in water displaying a maximal relative thermal sensitivity (S_r) of 1.53%·°C⁻¹ at 60 °C. Full article

Background/Objectives: Cadaveric models have traditionally been a mainstay of dental and medical education worldwide since their inception. In Australia, educators at dental schools were among the first to use cadaveric porcine heads in formal teaching in oral surgery. This practice has since fallen out of favour in most modern dental curricula. The aim of this pilot study was to determine the utility of cadaveric porcine models for oral surgery training from a student perspective (Griffith University, Gold Coast, Australia). Methods: Thirty participants who were all third-year dental students attended a two-hour session comprising a 30 min lecture followed by a 90 min practical workshop. The lecture outlined the steps and supervision of students during the practical and was provided by a consultant maxillofacial surgeon. At the conclusion of the workshop, participants were asked to anonymously complete a printed questionnaire with eight questions related to their experience. Results: Prior to the workshop, two-thirds (61%) of participants felt that they had been taught the surgical procedure for raising mucoperiosteal flaps adequately in their dental school curriculum during their third year, although only 43% of students had assisted specialty residents in raising a mucoperiosteal flap and 14% reported having performed the procedure themselves. Almost all students (96%) agreed that the porcine model was useful for their dental education and that they would practice the exercise using the model again if provided with the opportunity. The questionnaire had a 93.33% completion rate. Conclusions: This pilot study indicates that porcine heads present a useful, low-cost adjunct in the learning of basic oral surgical procedures. Full article

The work proves that under conditions of instability of the second derivatives of the function in the minimization region, the estimate of the convergence rate of Newton’s method is determined by the parameters of the irreducible part of the conditionality degree of the problem. These parameters represent the degree of difference between eigenvalues of the matrices of the second derivatives in the coordinate system, where this difference is minimal, and the resulting estimate of the convergence rate subsequently acts as a standard. The paper studies the convergence rate of the relaxation subgradient method (RSM) with optimization of the parameters of two-rank correction of metric matrices on smooth strongly convex functions with a Lipschitz gradient without assumptions about the existence of second derivatives of the function. The considered RSM is similar in structure to quasi-Newton minimization methods. Unlike the latter, its metric matrix is not an approximation of the inverse matrix of second derivatives but is adjusted in such a way that it enables one to find the descent direction that takes the method beyond a certain neighborhood of the current minimum as a result of one-dimensional minimization along it. This means that the metric matrix enables one to turn the current gradient into a direction that is gradient-consistent with the set of gradients of some neighborhood of the current minimum. Under broad assumptions on the parameters of transformations of metric matrices, an estimate of the convergence rate of the studied RSM and an estimate of its ability to exclude removable linear background are obtained. The obtained estimates turn out to be qualitatively similar to estimates for Newton’s method. In this case, the assumption of the existence of second derivatives of the function is not required. A computational experiment was carried out in which the quasi-Newton BFGS method and the subgradient method under study were compared on various types of smooth functions. The testing results indicate the effectiveness of the subgradient method in minimizing smooth functions with a high degree of conditionality of the problem and its ability to eliminate the linear background that worsens the convergence. Full article

The goal of this study was to provide antioxidant and antibacterial properties to different types of fabrics via tannic acid (TA) covalent grafting. To that extent, TA was first methacrylated using glycidylmethacrylate. TA derivatives were characterized using infrared spectroscopy and ¹H NMR to assess the degree of acrylation. Antioxidant and antibacterial properties of TA were preserved after chemical modification. The coating process was studied using infrared spectroscopy (IR), weight gain, and radical scavenging activity (RSA) measurements. To covalently bond TA to raw polypropylene (PP) and PP coated with chitosan, photoinduced grafting was performed. The process was optimized and resulted in fabrics with enough tannic acid to provide strong antioxidant activity, with RSA ranging at 95%. The antibacterial activity was assessed against E. coli and S. aureus, the main strains responsible for nosocomial infections. Results revealed a substantial reduction of bacterial contamination for PP samples coated with chitosan, with stronger activity against E. coli, attributed to hydrophobic repellence. This study highlights the benefits of using tannic acid to obtain antioxidant and antibacterial fabrics. Full article

This study investigates panorama photogrammetry (PPh) as a potential method to collect massive 3D information for long-range deformation monitoring. Particularly, this study focuses on areas with measuring restrictions, i.e., inaccessible objects and distances above 0.6 km. Under these particular conditions, geodetic techniques based on Electromagnetic Distance Meters (EDMs) or Total Stations (TSs) can provide coordinates with a precision better than 1 cm, but only for a limited number of discrete points. For mass capture, Terrestrial Laser Scanning (TLS) is normally the preferred solution, but long-range instruments are expensive, and drawbacks such as weak return signals and non-automatic target recognition appear. As an alternative, PPh is investigated in the well-controlled area of La Muela in Cortes de Pallas, where images are automatically captured from geodetic pillars using a GigaPan device, processed, and then rigorously compared to TLS point clouds. The results obtained after integrating both techniques into a high-accuracy geodetic reference frame show that PPh and TLS provide similar precision to within approximately 4 cm in the range of 0.6–1.0 km. Therefore, considering cost-effectiveness and ease of use, the proposed method can be considered a low-cost alternative to TLS for long-range deformation monitoring. Full article

The antibacterial microcapsules were prepared by encapsulating Toddalia asiatica (L.) Lam. extracts with urea–formaldehyde resin. The orthogonal test was designed to investigate the effects of the mass ratio of core and wall materials (W_core:W_wall), emulsifier concentration, reaction temperature and reaction time on the yield rate and coverage rate of microcapsules, and to obtain the best preparation technology for microcapsules. The single-factor results indicated that the maximum influence factor was the W_core:W_wall of the microcapsules; the larger the W_core:W_wall, the easier the microcapsules were to agglomerate; and when the W_core:W_wall was 0.8:1, the coverage rate reached the maximum value of 11.0%. The waterborne topcoat paint film was prepared by adding the microcapsules in the same content. The yield rate, coverage rate, and microscopic morphology of Toddalia asiatica (L.) Lam. extract microcapsules were analyzed, as well as the effects of microcapsules on the microscopic morphology, optical properties, cold liquid resistance, mechanical properties and antibacterial properties of a waterborne topcoat paint film. Combining the optical properties, cold liquid resistance, physical properties, and antibacterial properties of the waterborne topcoat paint film, the comprehensive performance of the waterborne topcoat paint film with the W_core:W_wall of 0.8:1 was superior. The gloss was 8.07 GU, color difference ΔE was 9.21, visible light transmittance was 82.90%, resistance to citric acid, ethanol and detergent were grade 1, 2 and 2, respectively, elongation at break was 15.68%, and roughness was 3.407 µm. The antibacterial activity against Escherichia coli and Staphylococcus aureus were 42.82% and 46.05%, respectively. In this study, a waterborne topcoat paint film with a microcapsule-coated plant-derived antibacterial agent as the core was prepared, expanding the application prospect of plant-derived antibacterial microcapsules. Full article

Numerous studies have been performed on Helicobacter pylori infection because of the high death rate linked to this illness and gastric cancer. An update on the key developments in recent years in the investigation of Helicobacter pylori and gastric cancer is the goal of this review. Using the search term “Helicobacter pylori, gastric cancer”, the PubMed database was searched. Only papers published in 2024 fulfilled the inclusion criteria. Because case report papers were not part of our investigation, they satisfied the exclusion criteria. Most of the research on the variable genes of Helicobacter pylori is guided by genetics to determine potential treatments. Studies on clinical treatments for the eradication of H. pylori with promising therapeutic options are needed. We found the fewest studies related to the immunopathology of H. pylori infection, which is still unknown. In conclusion, priority should be given to this kind of research. Full article

Resveratrol is a natural polyphenol with important anti-inflammatory and antioxidant properties for treating cardiometabolic disorders. Therefore, the present meta-analysis aimed to review and investigate the oral resveratrol supplementation effects on metabolic syndrome (MetS) components. The bibliographic search was carried out in 2023 in the following databases: PubMed, Web of Science, and Scopus. Studies that investigated the oral resveratrol effects on the MetS parameters were included. Statistical analyses were performed using RevMan Software V.5.3. The main findings showed that resveratrol significantly decreased systolic and diastolic blood pressure while having no significant effects on waist circumference and high-density lipoprotein levels. In addition, glucose level was significantly decreased in the subgroup of studies reporting change from baseline means, although the overall effect was not statistically significant (p = 0.81), while triglyceride levels were increased after the treatment period. In conclusion, the present meta-analysis evidenced the potential therapeutic effect of resveratrol on improving some MetS features, especially regarding systolic blood pressure, diastolic blood pressure, and glucose reduction; however, the results are still borderline and sometimes controversial, which might be justified by the methodological and statistical heterogeneity of the studies, with the latter varying from 17 to 57%. Full article

In this work, comb homopolymers as well as comb-type copolymers of thermo-responsive oligo(ethylene glycol methyl ether methacrylate)s, OEGMAs, with various chain lengths (DEGMA, PEGMA₅₀₀, and PEGMA₉₅₀ containing 2, 9, or 19 repeating ethylene glycol units, respectively) were synthesized through free radical (co)polymerization. For the copolymers, either the functional hydrophobic glycidyl methacrylate (GMA) or the inert hydrophilic N,N-dimethylacrylamide (DMAM) were selected as comonomers. The self-assembly and thermo-responsive behavior of the products was investigated through Nile Red fluorescence probing, turbidimetry, and dynamic light scattering (DLS). Interestingly, it was found that all OEGMA-based homopolymers exhibit a tendency to self-organize in aqueous media, in addition to thermo-responsiveness. The critical aggregation concentration (CAC) increases with the number of repeating ethylene oxide units in the OEGMA macromonomers (CAC was found to be 0.003, 0.01, and 0.03% w/v for the homopolymers PDEGMA, PPEGMA₅₀₀, and PPEGMA₉₅₀, respectively). Moreover, the CAC of the copolymers in aqueous media is highly affected by the incorporation of hydrophobic GMA or hydrophilic DMAM units, leading to lower or higher values, respectively. Thus, the CAC decreases down to 0.003% w/v for the GMA-richest copolymer of PEGMA₉₅₀, whereas CAC increases up to 0.01% w/v for the DMAM-richest copolymer of DEGMA. Turbidimetry and DLS studies proved that the thermo-sensitivity of the polymers is governed by several parameters such as the number of repeating ethylene glycol groups in the side chains of the OEGMAs, the molar percentage of the hydrophobic or hydrophilic comonomers, along with the addition of salts in the aqueous polymer solutions. Thus, the cloud point of the homopolymer PDEGMA was found at 23 °C and it increases to 33.5 °C for the DMAM-richest copolymer of DEGMA. Lastly, the formation of a hydrogel upon heating aqueous mixtures of the GMA-comprising copolymers with silica nanoparticles overnight is strong evidence of the functional character of these polymers. Full article

Glomerular diseases (GDs), significant causes of end-stage kidney disease, are better understood through epidemiological studies based on kidney biopsies (KBs), which provide important insights into their prevalence and characteristics. This study aims to analyze the clinicopathological features of GDs diagnosed from 2008 to 2017 at Romania’s largest reference center. In this decade-long study, 1254 adult patients diagnosed with GDs were included. The local previously validated renal histopathological prognostic score was calculated for each KB using four histopathologic lesions: global glomerulosclerosis, tubular atrophy, interstitial fibrosis and fibrocellular/fibrous crescents. The mean patient age was 50 years, with a male predominance (57%). The primary referral reasons were nephrotic syndrome (46%), nephritic syndrome (37%), chronic kidney disease (12%), asymptomatic urinary abnormalities (4%), and acute kidney injury (1%). Immunoglobulin A nephropathy (IgAN) was the most frequently diagnosed GD (20%), aligning with frequencies reported in European registries. Diabetic glomerular nephropathy was the most common secondary GD (10%). It also presented the highest median renal histopathological prognostic score (2), indicating a poorer prognosis. Lower eGFR and higher proteinuria were independently associated with higher scores. This decade-long study highlights IgAN as the most frequent GD diagnosed by KB. Diabetic glomerular nephropathy was identified as the most common secondary GD. The renal histopathological prognostic score, notably high in diabetic glomerular nephropathy patients, was correlated with lower eGFR and higher proteinuria, underlining its clinical relevance. Full article

6-BA, a small molecule compound of cytokinins, has been proven to delay leaf senescence in different species, including Chinese flowering cabbage; however, its specific mechanism remains relatively unknown. In this study, the application of external 6-BA delayed leaf senescence in Chinese flowering cabbage, showing that 6-BA effectively prevented the decrease in the maximum quantum yield (Fv/Fm) and overall chlorophyll content and suppressed the expression of the senescence-associated gene BrSAG12 over a 7-day period of storage. Moreover, treatment with 6-BA decreased the respiratory rate, NAD(H) content, the activities of hexose phosphate isomerase (PHI), succinate dehydrogenase (SDH), cytochrome c oxidase (CCO), and ascorbic acid oxidase (AAO) using enzyme-linked immunosorbent assay, and the transcriptional abundance of related genes by real-time quantitative polymerase chain reaction. Furthermore, 6-BA also increased the activity and expression levels of glucose-6-phosphate dehydrogenase (G6PDH) and 6-phosphate gluconate dehydrogenase (6-PGDH). The group treated with 6-BA retained elevated levels of NADP (H), ATP, total ATPase, and nicotinamide adenine dinucleotide kinase (NADK) activity, as well as the expression of respiratory enzymes. Molecular docking indicated that 6-BA hinders the glycolysis pathway (EMP), tricarboxylic acid cycle (TCA), and cytochrome pathway (CCP), and sustains elevated levels of the pentose phosphate pathway (PPP) through interactions with the PHI, SDH, 6-PGDH, G6PDH, CCO, and AAO proteins, consequently delaying postharvest leaf senescence in Chinese flowering cabbage. Full article

The growing need for materials that are eco-friendly and sustainable in the industrial sector has shifted focus from synthetic fossil to natural fibers, alongside the utilization of recycled polymer textiles. This research introduces a novel method for using end-of-life textiles, such as polyester and polyamide fabrics, in the production of composite materials, aiming to lessen textile waste and enhance material longevity. The mechanical attributes of flax fabric (FF), flax–recycled polyamide fabric (F/RPA), and flax–recycled polyester fabric (F/RPES) composite laminates are assessed through tensile, flexural, interlaminar shear, and Charpy impact tests. The study revealed that the addition of end-of-life synthetic fibers improves tensile strength, while the trend in modulus values suggests that flax provides a high degree of stiffness to the composites, which is moderated by the addition of synthetic fibers. This effect is consistent across both tensile and flexural testing, although the impact on stiffness is more significant in bending. The inclusion of polyester fibers in the composite laminate resulted in significant enhancements, with an 11.1% increase in interlaminar shear maximum force, a 17.4% improvement in interlaminar shear strength, and a 67.1% rise in un-notch impact energy, compared to composites made with only flax fiber (FF). The microscopic examination uncovered the internal structure and demonstrated a clear, strong bond between the polyester and polyamide fiber layers with the flax fibers. Additionally, the life cycle assessment revealed that the F/RPES composite had less environmental impact than FF and F/RPA in all 18 categories analyzed. This indicates that the environmental footprint of producing F/RPES is smaller than that of both FF and F/RPA. Full article

Locoregionally advanced and metastatic melanoma represent a challenging clinical problem, but in the era of immune checkpoint blockade and intralesional and infusional therapies, more options are available for use. Isolated limb infusion (ILI) was first introduced in the 1990s for the management of advanced melanoma, followed by the utilization of isolated extremity perfusion (ILP). Following this, intralesional oncolytic viruses, xanthene dyes, and cytokines were introduced for the management of in-transit metastases as well as unresectable, advanced melanoma. In 2015, the Food and Drug Administration (FDA) approved the first oncolytic intralesional therapy, talimogene laherparepvec (T-VEC), for the treatment of advanced melanoma. Additionally, immune checkpoint inhibition has demonstrated efficacy in the management of advanced melanomas, and this improvement in outcomes has been extrapolated to aid in the management of in-transit metastatic disease. Finally, percutaneous hepatic perfusion (PHP), also approved by the FDA, has been reported to have a significant impact on the treatment of hepatic disease in uveal melanoma. While some of these treatments have less utility due to inferior outcomes as well as higher toxicity profiles, there are selective patient profiles for which these therapies carry a role. This review highlights intralesional and infusional therapies for the management of metastatic melanoma. Full article

This paper presents an overview of integrating new research outcomes into the development of a structural health monitoring strategy for the floating cover at the Western Treatment Plant (WTP) in Melbourne, Australia. The size of this floating cover, which covers an area of approximately 470 m × 200 m, combined with the hazardous environment and its exposure to extreme weather conditions, only allows for monitoring techniques based on remote sensing. The floating cover is deformed by the accumulation of sewage matter beneath it. Our research has shown that the only reliable data for constructing a predictive model to support the structural health monitoring of this critical asset is obtained directly from the actual floating cover at the sewage treatment plant. Our recent research outcomes lead us towards conceptualising an advanced engineering analysis tool designed to support the future creation of a digital twin for the floating cover at the WTP. Foundational work demonstrates the effectiveness of an unmanned aerial vehicle (UAV)-based photogrammetry methodology in generating a digital elevation model of the large floating cover. A substantial set of data has been acquired through regular UAV flights, presenting opportunities to leverage this information for a deeper understanding of the interactions between operational conditions and the structural response of the floating cover. This paper discusses the current findings and their implications, clarifying how these outcomes contribute to the ongoing development of an advanced digital twin for the floating cover. Full article

Opportunistic networks, an evolution of mobile Ad Hoc networks (MANETs), offer decentralized communication without relying on preinstalled infrastructure, enabling nodes to route packets through different mobile nodes dynamically. However, due to the absence of complete paths and rapidly changing connectivity, routing in opportunistic networks presents unique challenges. This paper proposes a novel probabilistic routing model for opportunistic networks, leveraging nodes’ meeting probabilities to route packets towards their destinations. Thismodel dynamically builds routes based on the likelihood of encountering the destination node, considering factors such as the last meeting time and acknowledgment tables to manage network overload. Additionally, an efficient message detection scheme is introduced to alleviate high overhead by selectively deleting messages from buffers during congestion. Furthermore, the proposed model incorporates cross-layer optimization techniques, integrating optimization strategies across multiple protocol layers to maximize energy efficiency, adaptability, and message delivery reliability. Through extensive simulations, the effectiveness of the proposed model is demonstrated, showing improved message delivery probability while maintaining reasonable overhead and latency. This research contributes to the advancement of opportunistic networks, particularly in enhancing connectivity and efficiency for Internet of Things (IoT) applications deployed in challenging environments. Full article

Catalase (CAT), glutathione peroxidase (GPx), and peroxiredoxin 2 (Prx2) can counteract the deleterious effects of oxidative stress (OS). Their binding to the red blood cell (RBC) membrane has been reported in non-immune hemolytic anemias (NIHAs). Our aim was to evaluate the relationships between CAT, GPx, and Prx2, focusing on their role at the RBC membrane, in hereditary spherocytosis (HS), sickle cell disease (SCD), β-thalassemia (β-thal), and healthy individuals. The studies were performed in plasma and in the RBC cytosol and membrane, evaluating OS biomarkers and the enzymatic activities and/or the amounts of CAT, GPx, and Prx2. The binding of the enzymes to the membrane appears to be the primary protective mechanism against oxidative membrane injuries in healthy RBCs. In HS (unsplenectomized) and β-thal, translocation from the cytosol to the membrane of CAT and Prx2, respectively, was observed, probably to counteract lipid peroxidation. RBCs from splenectomized HS patients showed the highest membrane-bound hemoglobin, CAT, and GPx amounts in the membrane. SCD patients presented the lowest amount of enzyme linkage, possibly due to structural changes induced by sickle hemoglobin. The OS-induced changes and antioxidant response were different between the studied NIHAs and may contribute to the different clinical patterns in these patients. Full article

Excessive emissions of carbon dioxide have led to the greenhouse effect and global warming. Reducing carbon dioxide into high-value-added chemicals through solar energy is a promising approach. Herein, a g-C₃N₄/TiO₂ heterojunction photocatalyst with efficient electron transfer is designed for photocatalytic CO₂ reduction. The CH₄ (18.32 µmol·h⁻¹·g⁻¹) and CO (25.35 µmol·h⁻¹·g⁻¹) evolution rates of g-C₃N₄/TiO₂ are higher than those of g-C₃N₄ and TiO₂. The enhanced photocatalytic CO₂ reduction performance is attributed to the efficient charge carrier transfer in the g-C₃N₄/TiO₂ heterojunction. The electron transfer route was verified by in situ irradiated X-ray photoelectron spectroscopy (XPS). The photocatalytic CO₂ reduction mechanism on g-C₃N₄/TiO₂ was investigated by in situ diffuse reflectance infrared Fourier transform spectroscopy (DRIFTS). This work provides a strategy for designing a polymer/metallic oxide heterojunction with efficient electron transfer for enhanced photocatalytic CO₂ reduction. Full article