Cardoso, Isabel; Meissner, Anneke; Sawicki, Anja; Bartsch, Inka; Valentin, Klaus; Steinhagen, Sophie; Buck, Bela Hieronymus; Hofmann, Laurie C (2023): Salinity as a tool for strain selection in recirculating land-based production of Ulva spp. as adults [dataset]. PANGAEA, https://doi.org/10.1594/PANGAEA.956990
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Abstract:
In the paper "Salinity as a tool for strain selection in recirculating land-based production of Ulva spp. from germlings to adults" (Cardoso et al. 2023) the relative growth rates of adult thalli of different Ulva species and strains were measured to evaluate the impact of different salinity treatments. We hypothesised that salinity impacts the of the seaweeds and that different strains are adapted to different salinities. With this data we aimed to use salinity as a tool for selecting the strain with the highest and determine the optimal salinity to grow it in the future in a recirculating land-based system. Four strains were tested (U. lacinulata and U. linza from the NE-Atlantic and U. lacinulata and U. flexuosa from the Mediterranean). The NE- Atlantic strains were collected in the Óbidos Lagoon, Portugal in January 2021 and were cultivated in laboratory conditions in the Alfred Wegener Institute (AWI), Bremerhaven, Germany. The Mediterranean strains belong to an AWI collection and were isolated in 1986 and 1987 (U. flexuosa and U. lacinulata, respectively). The cultivation of the Mediterranean species with the purpose of using them for experiments started in June 2021. To recreate the conditions of a large-scale system, the strains were grown previously and during the experiment in a medium of artificial seawater (Seequasal-Salz, Seequasal Salz Production and Trade GmbH, Münster, Germany) enriched with a cheaper commercial fertilizer Blaukorn (COMPO SANA®, Germany). A uniform amount of fresh thalli from each species and population were placed into 1 L glass beakers with salinities of 10, 15, 20 and 30 PSU (each condition n = 3) and cultivated over 3 weeks. The fresh weight was measured once a week by collecting the seaweed and removing the excess water with absorbent paper three times before weighing the samples (Sartorius, Germany). Every time, each sample was measured 3 times. Based on the fresh weight measured each week, it was possible to calculate the of each strain throughout the experiment.
Keyword(s):
Related to:
Cardoso, Isabel; Meissner, Anneke; Sawicki, Anja; Bartsch, Inka; Valentin, Klaus; Steinhagen, Sophie; Buck, Bela Hieronymus; Hofmann, Laurie C (2023): Comparison of the nutrient concentration between Artificial and Natural Seawater. PANGAEA, https://doi.org/10.1594/PANGAEA.956857
Cardoso, Isabel; Meissner, Anneke; Sawicki, Anja; Bartsch, Inka; Valentin, Klaus; Steinhagen, Sophie; Buck, Bela Hieronymus; Hofmann, Laurie C (2023): Salinity as a tool for optimizing the Antioxidant Activity of Ulva lacinulata. PANGAEA, https://doi.org/10.1594/PANGAEA.956988
Cardoso, Isabel; Meissner, Anneke; Sawicki, Anja; Bartsch, Inka; Valentin, Klaus; Steinhagen, Sophie; Buck, Bela Hieronymus; Hofmann, Laurie C (2023): Salinity as a tool for strain selection in recirculating land-based production of Ulva spp. as germlings. PANGAEA, https://doi.org/10.1594/PANGAEA.956903
Cardoso, Isabel; Meissner, Anneke; Sawicki, Anja; Bartsch, Inka; Valentin, Klaus; Steinhagen, Sophie; Buck, Bela Hieronymus; Hofmann, Laurie C (2023): The impact of different nutrient sources in the of different Ulva strains. PANGAEA, https://doi.org/10.1594/PANGAEA.956989
References:
Cardoso, Isabel; Meiβner, Anneke; Sawicki, Anja; Bartsch, Inka; Valentin, Klaus; Steinhagen, Sophie; Buck, Bela Hieronymus; Hofmann, Laurie C (2023): Salinity as a tool for strain selection in recirculating land-based production of Ulva spp. from germlings to adults. Journal of Applied Phycology, https://doi.org/10.1007/s10811-023-02960-x
Project(s):
Coverage:
Median Latitude: 39.980497 * Median Longitude: 6.872087 * South-bound Latitude: 39.394977 * West-bound Longitude: -9.213583 * North-bound Latitude: 40.566018 * East-bound Longitude: 22.957756
Date/Time Start: 2021-01-04T12:00:00 * Date/Time End: 2021-01-04T12:00:00
Minimum DEPTH, water: 0 m * Maximum DEPTH, water: 0 m
Event(s):
AWI_Bremerhaven_FunctEcologyLab * Latitude: 53.533401 * Longitude: 8.580547 * Date/Time Start: 2021-10-12T00:00:00 * Date/Time End: 2022-04-11T00:00:00 * Location: Bremerhaven, Germany * Method/Device: Laboratory experiment * Comment: Functional Ecology Laboratory
Comment:
We have written confirmation by the Instituto da Conservação da Natureza e das Florestas (ICNF) in its function as ABS National Focal Point as well as Competent National Authority that although Portugal is party to the Nagoya Protocol no national legislation nor any regulatory requirements drawing from the Nagoya Protocol for access to genetic resources in mainland Portugal exist presently. As the samples for this project were collected in mainland Portugal, there are no applicable prior informed consent requirements. The Mediterranean Ulva material was isolated in 1986 (AWI culture number 1262) and in 1967 (AWI culture number 1290). Hence, the samples were taken before the Nagoya Protocol came into force in 2014. Although Regulation EU-No. 511/2014 does not apply accordingly, we complied with our due diligence by asking the Greek National Focal Point about national ABS permit requirements and were granted access with a research permit for flora (RECALL/Δ PΔ/12548/797) issued by the Ministry of The Environment & Energy- Gdd & Dp - Forest Protection Directorate).
Parameter(s):
# | Name | Short Name | Unit | Principal Investigator | Method/Device | Comment |
---|---|---|---|---|---|---|
1 | Event label | Event | Hofmann, Laurie C | |||
2 | Type of study | Study type | Hofmann, Laurie C | |||
3 | DATE/TIME | Date/Time | Hofmann, Laurie C | Geocode | ||
4 | Sampling date | Sampling date | Hofmann, Laurie C | |||
5 | LATITUDE | Latitude | Hofmann, Laurie C | Geocode | ||
6 | LONGITUDE | Longitude | Hofmann, Laurie C | Geocode | ||
7 | Origin | Origin | Hofmann, Laurie C | of sample | ||
8 | DEPTH, water | Depth water | m | Hofmann, Laurie C | Geocode – Mediterranean species were not collected by the investigators in this work. These species were provided to us in 2021. The NE-Atlantic species were collected by the principal investigator during low tide in a Lagoon were there was no water (at that specific time) | |
9 | Laboratory | Lab | Hofmann, Laurie C | |||
10 | Date/time start | Date/time start | Hofmann, Laurie C | of experiment | ||
11 | Date/time end | Date/time end | Hofmann, Laurie C | of experiment | ||
12 | Experiment duration | Exp duration | h | Hofmann, Laurie C | ||
13 | Species | Species | Hofmann, Laurie C | |||
14 | Sample code/label | Sample label | Hofmann, Laurie C | |||
15 | Accession number, genetics | Access no gen | Hofmann, Laurie C | NCBI; of tufA gene; Sanger sequencing of the purified amplicons was performed by the company Eurofins Genomics (Konstanz, Germany). Forward and reverse sequence reads were assembled in the DNA sequence analysis software Sequencher (version 4.1.4, Gene Codes Corporation). Using the BLAST function in GenBank, initial identifications using the specimens' tufA sequences were made. | ||
16 | Replicate | Repl | Hofmann, Laurie C | |||
17 | Number of measurements | n | # | Hofmann, Laurie C | Repeated measurements | |
18 | Treatment: salinity | T:sal | Hofmann, Laurie C | Refractometer, Atago, S-10E | PSU | |
19 | Temperature, water | Temp | °C | Hofmann, Laurie C | Temperature data logger, Ebro, EBI 20-T1 | |
20 | Treatment: light:dark cycle | T:L:D | hh:hh | Hofmann, Laurie C | ||
21 | Light intensity | Io | µmol/m2/s | Hofmann, Laurie C | Light meter, LI-COR Biosciences GmbH, LI-250A | |
22 | Nutrient | Nutrient | Hofmann, Laurie C | Fertilizer: Blaukorn (COMPO SANA®, Germany) | ||
23 | Concentration | Conc | ml/l | Hofmann, Laurie C | of nutrient | |
24 | Biomass, wet mass | Biom wm | g | Hofmann, Laurie C | Analytical Balance, Sartorius, LA310S | 3 measurements were made one after the other to guarantee more accuracy in the fresh weight of the seaweed |
License:
Creative Commons Attribution 4.0 International (CC-BY-4.0)
Status:
Curation Level: Enhanced curation (CurationLevelC)
Size:
10656 data points
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