Cardoso, Isabel; Meissner, Anneke; Sawicki, Anja; Bartsch, Inka; Valentin, Klaus; Steinhagen, Sophie; Buck, Bela Hieronymus; Hofmann, Laurie C (2023): Salinity as a tool for optimizing the Antioxidant Activity of Ulva lacinulata [dataset]. PANGAEA, https://doi.org/10.1594/PANGAEA.956988
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Abstract:
In the paper "Salinity as a tool for strain selection in recirculating land-based production of Ulva spp. from germlings to adults" (Cardoso et al. 2023) the antioxidant activity (AA) experiment aimed to test a previously selected strain, Ulva lacinulata from the NE-Atlantic, and evaluate the variations in AA under different salinity treatments. We hypothesized that by reducing the salinity level in the recirculating land-based system, it would be possible to optimize and increase the quality of the biomass being produced before it being harvested. The NE-Atlantic seaweed was collected in the Óbidos Lagoon, Portugal in January 2021 and was cultivated in laboratory conditions in the Alfred Wegener Institute (AWI), Bremerhaven, Germany. To recreate the conditions of a large-scale system, the strain was grown previously and during the experiment in a medium of artificial seawater (Seequasal-Salz, Seequasal Salz Production and Trade GmbH, Münster, Germany) enriched with a cheaper commercial fertilizer Blaukorn (COMPO SANA®, Germany). Twelve 1 L beakers were filled with artificial seawater at 4 different salinities (10, 15, 20 and 30 PSU), measured with a refractometer (Atago, Japan) (n = 3). Six discs (2 cm) of U. lacinulata were placed in each beaker and, at different times (0 h, 3 h, 24 h, 120 h, 192 h and 240 h) 1 disc was collected from each beaker to evaluate the antioxidant activity. The AA was determined by the ABTS Radical Cation Decolourisation Assay based on Re et al., 1999, and a Trolox standard curve was created by measuring the absorption of different Trolox concentrations in ethanol after being mixed with ABTS. The absorption of the samples at 734 nm was analysed with a microplate reader (Infinite 200 Microplate Reader, Tecan Trading AG, Männedorf, Switzerland) and the AA results obtained were given in Trolox Equivalent (in µg/mL).
Keyword(s):
Related to:
Cardoso, Isabel; Meissner, Anneke; Sawicki, Anja; Bartsch, Inka; Valentin, Klaus; Steinhagen, Sophie; Buck, Bela Hieronymus; Hofmann, Laurie C (2023): Comparison of the nutrient concentration between Artificial and Natural Seawater. PANGAEA, https://doi.org/10.1594/PANGAEA.956857
Cardoso, Isabel; Meissner, Anneke; Sawicki, Anja; Bartsch, Inka; Valentin, Klaus; Steinhagen, Sophie; Buck, Bela Hieronymus; Hofmann, Laurie C (2023): Salinity as a tool for strain selection in recirculating land-based production of Ulva spp. as adults. PANGAEA, https://doi.org/10.1594/PANGAEA.956990
Cardoso, Isabel; Meissner, Anneke; Sawicki, Anja; Bartsch, Inka; Valentin, Klaus; Steinhagen, Sophie; Buck, Bela Hieronymus; Hofmann, Laurie C (2023): Salinity as a tool for strain selection in recirculating land-based production of Ulva spp. as germlings. PANGAEA, https://doi.org/10.1594/PANGAEA.956903
Cardoso, Isabel; Meissner, Anneke; Sawicki, Anja; Bartsch, Inka; Valentin, Klaus; Steinhagen, Sophie; Buck, Bela Hieronymus; Hofmann, Laurie C (2023): The impact of different nutrient sources in the of different Ulva strains. PANGAEA, https://doi.org/10.1594/PANGAEA.956989
References:
Cardoso, Isabel; Meiβner, Anneke; Sawicki, Anja; Bartsch, Inka; Valentin, Klaus; Steinhagen, Sophie; Buck, Bela Hieronymus; Hofmann, Laurie C (2023): Salinity as a tool for strain selection in recirculating land-based production of Ulva spp. from germlings to adults. Journal of Applied Phycology, https://doi.org/10.1007/s10811-023-02960-x
Re, Roberta; Pellegrini, Nicoletta; Proteggente, Anna; Pannala, Ananth; Yang, Min; Rice-Evans, Catherine (1999): Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radical Biology and Medicine, 26(9-10), 1231-1237, https://doi.org/10.1016/S0891-5849(98)00315-3
Project(s):
Coverage:
Latitude: 39.394977 * Longitude: -9.213583
Date/Time Start: 2021-04-01T12:00:00 * Date/Time End: 2021-04-01T12:00:00
Minimum DEPTH, water: 0 m * Maximum DEPTH, water: 0 m
Event(s):
Comment:
We have written confirmation by the Instituto da Conservação da Natureza e das Florestas (ICNF) in its function as ABS National Focal Point as well as Competent National Authority that although Portugal is party to the Nagoya Protocol no national legislation nor any regulatory requirements drawing from the Nagoya Protocol for access to genetic resources in mainland Portugal exist presently. As the samples for this project were collected in mainland Portugal, there are no applicable prior informed consent requirements. The Mediterranean Ulva material was isolated in 1986 (AWI culture number 1262) and in 1967 (AWI culture number 1290). Hence, the samples were taken before the Nagoya Protocol came into force in 2014. Although Regulation EU-No. 511/2014 does not apply accordingly, we complied with our due diligence by asking the Greek National Focal Point about national ABS permit requirements and were granted access with a research permit for flora (RECALL/Δ PΔ/12548/797) issued by the Ministry of The Environment & Energy- Gdd & Dp - Forest Protection Directorate).
Parameter(s):
# | Name | Short Name | Unit | Principal Investigator | Method/Device | Comment |
---|---|---|---|---|---|---|
1 | Event label | Event | Hofmann, Laurie C | |||
2 | Type of study | Study type | Hofmann, Laurie C | |||
3 | DATE/TIME | Date/Time | Hofmann, Laurie C | Geocode | ||
4 | LATITUDE | Latitude | Hofmann, Laurie C | Geocode | ||
5 | LONGITUDE | Longitude | Hofmann, Laurie C | Geocode | ||
6 | Origin | Origin | Hofmann, Laurie C | of sample | ||
7 | DEPTH, water | Depth water | m | Hofmann, Laurie C | Geocode – The NE-Atlantic species were collected by the principal investigator during low tide in a Lagoon were there was no water (at that specific time) | |
8 | Laboratory | Lab | Hofmann, Laurie C | |||
9 | Date/time start | Date/time start | Hofmann, Laurie C | of experiment | ||
10 | Date/time end | Date/time end | Hofmann, Laurie C | of experiment | ||
11 | Experiment duration | Exp duration | h | Hofmann, Laurie C | ||
12 | Species | Species | Hofmann, Laurie C | |||
13 | Accession number, genetics | Access no gen | Hofmann, Laurie C | NCBI; of tufA gene; Sanger sequencing of the purified amplicons was performed by the company Eurofins Genomics (Konstanz, Germany). Forward and reverse sequence reads were assembled in the DNA sequence analysis software Sequencher (version 4.1.4, Gene Codes Corporation). Using the BLAST function in GenBank, initial identifications using the specimens' tufA sequences were made. | ||
14 | Replicate | Repl | Hofmann, Laurie C | |||
15 | Treatment: salinity | T:sal | Hofmann, Laurie C | Refractometer, Atago, S-10E | PSU | |
16 | Temperature, water | Temp | °C | Hofmann, Laurie C | Temperature data logger, Ebro, EBI 20-T1 | |
17 | Treatment: light:dark cycle | T:L:D | hh:hh | Hofmann, Laurie C | ||
18 | Light intensity | Io | µmol/m2/s | Hofmann, Laurie C | Light meter, LI-COR Biosciences GmbH, LI-250A | |
19 | Nutrient | Nutrient | Hofmann, Laurie C | Fertilizer: Blaukorn (COMPO SANA®, Germany) | ||
20 | Concentration | Conc | ml/l | Hofmann, Laurie C | of nutrient | |
21 | Antioxidant capacity, in Trolox Equivalents | TEAC | µg/ml | Hofmann, Laurie C | Microplate reader, Tecan Trading AG, Infinite 200 | A Trolox standard curve was created by measuring the absorption of different Trolox concentrations (0 - 100 µg mL-1) in ethanol after being mixed with ABTS. The Trolox calibration curve was then used to calculate the AA of the samples in Trolox equivalents TE in µg mL-1. |
License:
Creative Commons Attribution 4.0 International (CC-BY-4.0)
Status:
Curation Level: Enhanced curation (CurationLevelC)
Size:
1147 data points