Accessing Lysozyme Nucleation with a Novel Dynamic Light Scattering Detector

Dynamic light scattering was employed to investigate the behaviour of nucleating hen egg-white lysozyme solutions. For these studies a novel fiber-optic based microprobe that suppresses multiple light scattering and contributions from large clusters in the spectra by backscattering detection was employed. The time evolution of small lysozyme clusters was found to obey classical nucleation at the initial stages of the reaction.