Summary
Outwardly rectifying Cl− channels in cultured human Jurkat T-lymphocytes were activated by excising a patch of membrane using the inside-out (i/o) patch-clamp configuration and holding at depolarized voltages for prolonged periods of time (1–6 min at +80 mV, 20°C). The single-channel current at +80 mV was 4.5 ± 0.3 pA and at −80 mV, it was 1.0 ± 0.4 pA. After activation, the probability of being open (P 0)for the lymphocyte channel was voltage independent. Activation of the Cl− channel in lymphocytes was temperature dependent. Nineteen percent of i/o recordings from lymphocytes made at 20°C exhibited Cl− channel activity. In contrast, 49% of recordings made at 30°C showed channel activity. The number of channels in an active patch was not significantly different at the two temperatures. Channel activation in excised, depolarized patches also occurred 20-fold faster at 30°C than at 20°C. There was no marked change in the single-channel conductance at 30°C. Open-channel conductance was blocked by 200 μm indanyloxyacetic acid (IAA) or 1 mm SITS when applied to the intracellular side of the patch. The characteristics of this channel are similar to epithelial outwardly rectifying Cl− channels thought to be involved in fluid secretion
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Garber, S.S. Outwardly rectifying chloride channels in lymphocytes. J. Membarin Biol 127, 49–56 (1992). https://doi.org/10.1007/BF00232757
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DOI: https://doi.org/10.1007/BF00232757