Summary
Concentrations of phosphoadenylate nucleotides and the adenylate energy charge ((ATP+1/2ADP)/(ATP+ADP+AMP)) have been suggested as sensitive integrating measures of the energy state of organisms. This synoptic study investigated the seasonal and spatial variation of phosphoadenylate concentrations and AEC in two freshwater bivalve molluscs, the paper-shell clam, Anodonta imbecillis and the asian clam, Corbicula fluminea. Concentrations of all three adenylates, as well as the total adenylate concentration and adenylate energy charge of both species varied seasonally. These fluctuations were closely related to reproductive periods in both species. Total adenylate concentrations and ATP concentrations were slightly negatively correlated with shell length in A. imbecillis but the ADP and AMP concentrations and AEC were not significantly correlated with shell length. In C. fluminea the AEC was negatively correlated were positively correlated with shell length. Neither species exhibited significant differences in AEC between two collection locations. When C. fluminea collected from the Savannah River were acclimated and fed in the laboratory their AEC increased significantly.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Agnew JS, Sedo RJ, Atkinson DE (1973) Relative independence of adenylate energy charge and adenylate pool level in E. coli. Abstract Fed Proc 32:575
Ansell AD (1977) The adenosene triphosphate content of some marine bivalve molluscs. J Exp Mar Biol Ecol 28:269–283
Atkinson DE (1977) Cellular Energy Metabolism and its Regulation. Academic Press, New York p 293
Balch N (1972) ATP content of Calanus finmarchicus. Limnol Oceanogr 17:906–908
Bamstedt V, Skjoldal HR (1976) Studies on the deepwater pelagic community of Korstjarden, western Norway. Adenosine phosphates and nucleic acids in Euchaeta norwegica (Copepod) in relation to its life cycle. Sarsia 60:63–80
Barr AJ, Goodnight JH, Sall JP (1979) SAS User's Guide. Raleigh, North Carolina: SAS Institute, Inc p 294
Beis I, Newsholme EA (1975) The contents of adenine nucleotides, phosphagens and some glycolytic intermediates in resting muscles from vertebrates and invertebrates. Biochem J 152:23–32
Britton JC, Morton B (1977) Corbicula in North America. The evidence reviewed and evaluated. In: JC Britton, JS Mattice, CE Murphy, and LW Newland (eds), Proceedings, First international Corbicula Symposium, Texas Christian University, Fort Worth, TX, October 13–15, 1977. Texas Christian University Research Foundation. Forth Worth, TX p 249–287
Britton JC, Coldiron DR, Evans Jr, Golightly C, O'Kane DD, Teneyck JR (1979) Reevaluation of the growth pattern in Corbicula fluminea (Muller). In: Proceedings, First International Corbicula Symposium, Texas Christian University, Forth Worth, TX, October 13–15, 1977 JC Britton, CE Mattice, CE Murphy, and LW Newland (eds), Texas Christian University Research Foundation. Forth Worth, TX p 177–192
Buikema AL, Sherberger SR, Knauer GW, Newbern LA, Reading JT, Cairns J (1979) Effects of simulated entrainment on the biology of a freshwater cladoceran. In: JH Thorp and JW Gibbons (eds), Energy and Environmental Stress in Aquatic Systems, DOE Symposium Series 48, CONF-771114 p 809–825
Bull RJ, O'Neill JJ (1975) Spectral changes in the respiratory chain of cerebral cortex slices. Correlation with the energy status of the tissue. Psychopharmacology Communications 1:109–115
Burress RM, Chandler JH Jr (1976) Use of the Asiatic Clam, Corbicula leana Prime, in Toxicity Tests. Prog Fish Cult 38:10
Capuzzo JM, Sasner JJ (1977) The effect of chromium on filtration rates and metabolic activity of Mytilis edulis L. and Mya arenaria L. In Physiological Responses of Marine Biota to Pollutants, FJ Vernberg, A Calabrese, FP Thurberg and WB Vernberg (eds), Academic Press, New York pp 225–237
Chapman AG, Fall L, Atkinson DE (1971) Adenylate energy charge in Escherichia coli during growth and starvation. J Bacteriol 108:1072–1086
Dickson GW (1980) Phosphoadenylate concentrations and adenylate energy charge in freshwater crayfish (Decapoda:Astacidae). Natural and stress related variation. PhD Dissertation, University of Georgia, Athens, Ga p 112
Giesy JP, Denzer SR, Duke CS, Dickson GW (1981a) Phosphoadenylate concentrations and energy charge in two fresh-water crustaceans: Response to physical and chemical stressors. Verh Internat Verein Limnol 21:173–188
Giesy JP, Duke CS, Bingham RD, Dickson GW, Leversee GJ (1981b) Changes in phosphoadenylate concentrations and energy charge in the Asian Clam (Corbicula fluminea) due to starvation or chronic cadmium exposure. Society of Environmental Toxicology and Chemistry (in press)
Hiltz DF, Dyer WJ (1970) Principal acid-soluble nucleotides in adductor muscle of the scallop (Placopecten magellanicus) and their degradation during postmortem storage on ice. J Fish Res Bd Canada 27:83–92
Innes WT (1966) Exotic Aquarium Fishes. Maywood New Jersey: Metaframe Co
Ivanovici AM Adenylate energy charge (1979) Potential value as a tool for rapid determination of toxicity effects. p 241–255 In Proc 5th Ann Aquatic Toxicity Workshop, Hamilton Ontario, November 7–9, 1978. Fish Mar Serv Tech Rep p 862
Ivanovici AM (1980) The adenylate energy charge in the estuarine mollusc, Pyruzus ebeninus. I: Laboratory studies of responses to salinity and temperature. Comp Biochem Physiol 66A:43–55
Ivanovici AM, Wiebe WJ (1980) For a working definition of “stress”: a review and critique In: GW Barrett and R Rosenberg (eds), Stress and Natural Ecosystems. Wiley and Sons, New York (in press)
Kawai DK (1953) Carbonic anhydrase in pearl oyster. Changes of the enzyme activity in relation to growth and seasons. Publis Seto Marine Biol Lab Kyoto Japan: Kyoto Univ 5:89–94
Lane CE, Tierney JQ (1951) Hydrodynamics and respiration in Teredo. Bull Marine Sci Gulf and Caribbean 1:104–110
Morrison DF (1967) Multivariate Statistical Methods. McGraw-Hill Book Co., New York, NY p 338
Pennak RW (1978) Fresh-water Invertebrates of the United States, second edition. John Wiley and Sons, New York p 803
Rainer SF, Ivanovici AM, Wadley VA (1979) Effect of reduced salinity on adenylate energy charge in three estuarine molluscs. Mari Biol 54:91–99
Selye H (1976) Stress in Health and Disease. Butterworth, Inc Boston, Ma, p 334
Sickel JB (1979) Population dynamics of Corbicula in the Altamaha River, Georgia. In Proceedings, First International Corbicula Symposium, Texas Christian University, Fort Worth, TX, October 13–15, 1977. JC Britton, JS Mattice, CE Murphy, and LW Newland (eds), Texas Christian University Research Foundation Fort Worth, TX p 69–80
Skjoldal HR, Bamstedt U (1976) Studies of the deepwater pelagic community of Korstjorden, western Norway. Adenosine phosphates and nucleic acids in Meganyctiphanes norvegica (Euphausiacea) in relation to the life cycle. Sarsia 61:1–14
Sokal RR, Rohlf FJ (1969) Biometry, the principles and practice of statistics in biological research. WH Freeman and Co, San Francisco p 386–387
Stolinge MR (1978) Tables of the studentized augmented range and applications to problems of multiple comparisons. J Amer Stat Assoc 73:656–660
Wilbur KM, Anderson NG (1950) Carbonic anhydrase and growth in the oyster and busycon. Biol Bull 98:19–24
Wilbur KM, Owen G (1964) Growth. In Physiology of Mollusca Vol 1, KW Wilbur and CM Yonge (eds), Academic Press New York p 211–242
Wylie V, Smith M (1964) Nucleotides of the Pacific oyster Crassostrea gigas (Thunberg). Can J Biochem 42:1347–1351
Zeuthen E (1947) Body size and metabolic rate in the animal kingdom with special regard to the marine micro-fauna. Compt Rend Trav Lab Carlsberg Ser Chim 26:17–161
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Giesy, J.P., Dickson, G.W. The effect of season and location on phosphoadenylate concentrations and adenylate energy charge in two species of freshwater clams. Oecologia 49, 1–7 (1981). https://doi.org/10.1007/BF00376890
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00376890