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Amplification of the amyE-tmrB region on the chromosome in tunicamycin-resistant cells of Bacillus subtilis

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Summary

In a class of tunicamycin-resistant mutants (tmrA7) of Bacillus subtilis, the production of extracellular α-amylase is increase by about five fold. The tmrA7 characteristics (tunicamycin resistance and hyperproduction of extracellular α-amylase) can be transferred to recipient cells by transformation. In the transformants and the original tmrA7 mutant, typical amplification of the region from 4 kb upstream of the amyE gene to the tmrB gene on the chromosome was detected. The repeating unit, 16 kb in size, repeats tandemly about five and ten times in the mutant and transformants, respectively, and the α-amylase production is proportional to the copy number of the amyE gene. Simultaneous amplification of the tmrB gene, which is responsible for tunicamycin resistance in the multicopy state, and the α-amylase structural gene (amyE) seems to be the cause of the pleiotropy of the tmrA7 mutation.

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Author notes

  1. Shintaro Nomura & Gakuzo Tamura

    Present address: Department of Applied Biological Science, Science University of Tokyo, Yamazaki 2641, 278, Noda-shi, Chiba, Japan

Authors and Affiliations

  1. Department of Agricultural Chemistry, The University of Tokyo, 113, Bunkyo-ku, Tokyo, Japan

    Ken-ichi Hashiguchi, Akihiko Tanimoto, Koji Yoda, Shigeyoshi Harada, Masaki Mori, Akira Takatsuki, Makari Yamasaki & Gakuzo Tamura

  2. Institute of Applied Microbiology, The University of Tokyo, 113, Bunkyo-ku, Tokyo, Japan

    Shintaro Nomura

  3. Institute of Biological Sciences, University of Tsukuba, 305, Sakura, Ibaraki, Japan

    Kunio Yamane & Takashi Furusato

Authors
  1. Ken-ichi Hashiguchi
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  2. Akihiko Tanimoto
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  3. Shintaro Nomura
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  4. Kunio Yamane
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  5. Koji Yoda
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  6. Shigeyoshi Harada
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  7. Masaki Mori
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  8. Takashi Furusato
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  9. Akira Takatsuki
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  10. Makari Yamasaki
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  11. Gakuzo Tamura
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Communicated by M. Takanami

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Hashiguchi, Ki., Tanimoto, A., Nomura, S. et al. Amplification of the amyE-tmrB region on the chromosome in tunicamycin-resistant cells of Bacillus subtilis . Molec Gen Genet 204, 36–43 (1986). https://doi.org/10.1007/BF00330184

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  • DOI: https://doi.org/10.1007/BF00330184

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