Abstract
Guard-cell protoplasts were isolated by enzymic digestion of the epidermis peeled from the abaxial surface of leaves from Commelina communis L. The protoplasts were separated from mesophyll-cell protoplasts and other contaminants by density-gradient centrifugation, and the purity of the preparations carefully and quantitatively assessed by light microscopy. The preparations of guard-cell protoplasts were then compared with mesophyll-cell protoplasts in terms of the activity of photosystem II as assessed by a) the light-induced evolution of oxygen under both steady-state and flashing light and b) the characteristics of photosystem-II chlorophyll fluorescence. In all experiments, clear photosystem-II activity was found in guard-cell protoplasts, although some subtle distinctions between guard-cell and mesophyll-cell protoplasts were found. The contribution of any contaimination by mesophyll-cell chlorophyll to guard-cell-protoplast signals was estimated to be less than 3% in all cases. The results indicate that photosystem II is present and active in guard cells of Commelina.
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Abbreviations
- DCMU:
-
3-(3′, 4′-dichlorophenyl)-1,1-dimethylurea
- GCP:
-
guard-cell protoplasts
- GCC:
-
guard-cell chloroplasts
- MCP:
-
mesophyll-cell protoplasts
- MCC:
-
mesophyllcell chloroplasts
- PS:
-
photosystem
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Hipkins, M.F., Fitzsimons, P.J. & Weyers, J.D.B. The primary processes of photosystem II in purified guard-cell protoplasts and mesophyll-cell protoplasts from Commelina communis L.. Planta 159, 554–560 (1983). https://doi.org/10.1007/BF00409145
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DOI: https://doi.org/10.1007/BF00409145