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Regeneration of plants from leaf explants of Cucumis melo cv. Pusa Sharbati

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Abstract

Leaves of three different sizes excised from 14, 21, 28 and 35-day-old seedlings of Cucumis melo were cultured on a MS medium supplemented with a range and combination of growth regulators. Maximum shoot differentiation from the leaf explants occurred in the combined presence of BAP and 2iP at equimolar concentration of 1 μM. Regeneration potential of leaves declined with increasing size of the leaves and the age of the donor seedlings. For elongation the shoots were transferred to MS+BAP [1 μM]. Such shoots were rooted with 75% frequency on MS+IAA [0.5 μM]. The plants have been established in pots.

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Abbreviations

BM:

Basal Medium

MS:

Murashige and Skoog

BAP:

6-Benzyl amino-purine

2iP:

6- V, V -dimethylallylamino purine

IAA:

Indole-3-acetic acid

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Authors and Affiliations

  1. Department of Botany, University of Delhi, 110007, Delhi, India

    Rekha Kathal, S. P. Bhatnagar & Sant S. Bhojwani

Authors
  1. Rekha Kathal
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  2. S. P. Bhatnagar
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  3. Sant S. Bhojwani
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Communicated by I. K. Vasil

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Kathal, R., Bhatnagar, S.P. & Bhojwani, S.S. Regeneration of plants from leaf explants of Cucumis melo cv. Pusa Sharbati. Plant Cell Reports 7, 449–451 (1988). https://doi.org/10.1007/BF00269535

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  • DOI: https://doi.org/10.1007/BF00269535

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