Abstract
Papaver somniferum L. (opium poppy) cells were elicited with a Botrytis sp. homogenate and cultured by a semi-continuous process. Elicitation induced synthesis of sanguinarine and dihydrosanguinarine. Significant release of both alkaloids into the culture medium occurred. Medium exchange at 2-day intervals enabled product recovery from spent medium and maintained culture viability. Culture growth was not inhibited by elicitor treatment necessitating sub-culture prior to re-elicitation. Re-elicited cultures displayed an increasing sensitivity (reduced growth rate, higher alkaloid yield) to the elicitor with each successive treatment and did not survive a fourth elicitation.
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Abbreviations
- DW:
-
dry weight
- FW:
-
fresh weight
- 2,4-D:
-
2,4-dichlorophenoxyacetic acid
- SGE:
-
sanguinarine
- DSGE:
-
dihydrosanguinarine
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Communicated by F. Constabel
Publication 29143 from the National Research Council of Canada
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Tyler, R.T., Eilert, U., Rijnders, C.O.M. et al. Semi-continuous production of sanguinarine and dihydrosanguinarine by Papaver somniferum L. cell suspension cultures treated with fungal homogenate. Plant Cell Reports 7, 410–413 (1988). https://doi.org/10.1007/BF00269525
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DOI: https://doi.org/10.1007/BF00269525