Summary
The repair of double-strand gaps (DSGs) in the plasmid DNA of radiosensitive mutants of Saccharomyces cerevisiae has been analyzed. The proportion of repair events that resulted in complete plasmid DNA DSG recovery was close to 100% in Rad+ cells. Mutation rad55 does not influence the efficiency and preciseness of DSG repair. The mutant rad57, which is capable of recombinational DNA DSB repair, resulted in no DSG recovery. Mutation rad53 substantially inhibits the efficiency of DSG repair but does not influence the precision of repair. Plasmid DNA DSG repair is completely blocked by mutations rad50 and rad54.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Altherr MR, Quinn LA, Kado CI, Rodrigues RL (1983) In: Lurquin PF, Klienhofs A (ed) Genetic engineering in eukaryotes. Washington, p 33–36
Budd M, Mortimer RK (1982) Mutat Res 103:19–24
Chernoff YO, Kidgotko OV, Demberelijn O, Luchnikova IL, Soldatov SP, Glaser VM, Gordenin DA (1984) Curr Genet 9:31–37
Frankenberg-Schwager M, Frankenberg D, Blocher D, Adamczyk C (1980) Int J Radiol Biol 37:207–212
Game JC, Mortimer RK (1974) Mutat Res 24:281–292
Glaser VM, Samadashivili MP, Vishnevetskaya OV, Soldatov SP, Shestakov SV (1985) Mutat Res 147:296–297
Gordenin DA, Trofimova MV, Shaburova ON, Pavlov Yi, Chernoff YO, Chekuolene YU, Proscyavichus YY, Sasnauskas KV, Janulaitis AA (1988) Mol Gen Genet 213:388–393
Kunes S, Botstein D, Fox MS (1985) J Mol Biol 184:375–387
Maniatis T, Fritsch EF, Sambrook J (1982) Molecular cloning. A laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York
Orr-Weaver TL, Szostak JW, Rothstein RJ (1981) Proc Natl Acad Sci USA 78:6354–6358
Orr-Weaver TL, Szostak JW (1983) Proc Natl Acad Sci USA 80:4417–4421
Perera JR, Glasunov AV, Glaser VM, Boreiko AV (1988) Mol Gen Genet 213:421–424
Petin VG, Kabakova NM (1981) Mutat Res 82:285–294
Toh-E A, Tada S, Oshima Y (1982) J Bacteriol 151:1980–1990
Saeki T, Nashida I, Nakai S (1980) Mutat Res 73:251–265
Vishnevetskaya OYu, Luchnik AN, Arutunova LS, Shestakov SV (1983) Genetika 19:26–32
White C, Sedgwick S (1985) Mol Gen Genet 201:99–106
Author information
Authors and Affiliations
Additional information
Communicated by P. P. Slonimski
Rights and permissions
About this article
Cite this article
Glaser, V.M., Glasunov, A.V., Tevzadze, G.G. et al. Genetic control of plasmid DNA double-strand gap repair in yeast, Saccharomyces cerevisiae . Curr Genet 18, 1–5 (1990). https://doi.org/10.1007/BF00321107
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00321107