Abstract
We performed fed-batch and continuous fermentations to extend the time of maximal nikkomycin production by Streptomyces tendae Tü 901/S 2566. This was achieved by the fed-batch culture technique. Furthermore, high productivity was obtained at slow growth rates in a continuous fermentation process. Different dilution rates with and without carbon limitation were done and the results were compared.
Similar content being viewed by others
References
Becker JM, Covert NL, Shengbagamurthi P, Steinfeld AS, Naider F (1983) Polyoxin D inhibits growth of zoopathogenic fungi. Antimicrob Agents Chemother 23:926–929
Birch A, Häusler A, Hütter R (1990) Genome rearrangement and genetic instability in Streptomyces spp. J Bacteriol 172:4138–4142
Bormann C, Huhn W, Zähner H, Rathmann R, Hahn H, König WA (1985) Metabolic products of microorganisms. 228. New nikkomycins produced by mutants of Streptomyces tendae. J Antibiot 38:9–16
Bu'lock JD, Powell AJ (1965) Secondary metabolism: an explanation in terms of induced enzyme metabolism. Experientia 21:55–56
Bu'lock JD, Detroy RW, Hostalek Z, Munim-al-shakarchi A (1974) Regulation of secondary biosynthesis in Gibberella fujikuroi. Trans Br Mycol Soc 62:377–389
Crüger W, Frommer W, Goelker C, Kaiser JW, Moeschler HF, Salcher O, Schedel M, Wehlmann H (1985) Neue Pflanzenschutzwirkstoffe aus Mikroorganismen. Pflanzenschutznachr Bayer 38:305–348
Dähn U, Hagenmaier H, Höhne H, König WA, Wolf G, Zähner H (1976) Stoffwechselprodukte von Mikroorganismen 154. Mitteilung. Nikkomycin, ein neuer Hemmstoff der Chitinsynthese bei Pilzen. Arch Mikrobiol 107:143–160
Decker H, Zähner H, Heitsch H, König WA, Fiedler HP (1991) Structure-activity relationships of the nikkomycins. J Gen Microbiol 137:1805–1813
Dehler W (1979) Nikkomycine — Studien zur Fermentation und Isolierung. Dissertation, University of Tübingen
Delzer J, Fiedler HP, Müller H, Zähner H, Rathmann R, Ernst K, König WA (1984) New nikkomycins by mutasynthesis and directed fermentation. J Antibiot 37:80–82
Fiedler HP (1984) Screening for new microbial products by high-performance liquid chromatography using photodiode array detector. J Chromatogr 316:487–494
Fiedder HP, Kurth R, Langhärig J, Delzer J, Zähner H (1982) Nikkomycins: microbial inhibitors of chitin synthase. J Chem Technol Biotechnol 32:271–280
Hector R, Zimmer BL, Pappagianis D (1990) Evaluation of nikkomycins X and Z in murine models of coccidioidomycosis, histoplasmosis, and blastomycosis. Antimicrob Agents Chemother 34:587–593
Herbert D, Phibbs JP, Strange RE (1971) Chemical analysis of microbial cells. In: Norris JR, Ribbons DW (eds) Methods in microbiology, vol 5B. Academic Press, London, pp 209–344
Hohenwallner W, Wimmer E (1973) The malachite green micromethod for determination of inorganic phosphate. Clin Chim Acta 45:169–175
Itaya K, Ui M (1966) A new micromethod for the colorimetric determination of inorganic phosphate. Clin Chim Acta 14:361–366
Kurth R (1981) Untersuchungen zur Regulation der Nikkomycinbildung und Optimierung einer pO2-Feeding-Fermentation. Dissertation, University of Tübingen
Lilley G, Clark AE, Lawrence GC (1981) Control of the production of cephamycin C and thienamycin by Streptomyces cattleya NRRL 8057. J Chem Technol Biotechnol 31:127–134
Lohr D, Buschulte T, Gilles ED (1989) Continuous cultivation of Streptomyces tendae in different media. Appl Microbiol Biotechnol 32:274–279
Matteo CC, Cooney CL, Demain AL (1976) Production of gramicidin S synthetases by Bacillus brevis in continuous culture. J Gen Microbiol 96:415–422
Plaga A, Stümpfel J, Fiedler HP (1989) Determination of carbohydrates in fermentation processes by high performance liquid chromatography. Appl Microbiol Biotechnol 32:45–49
Roth M, Noack D (1982) Genetic stability of differentiated functions i in Streptomyces hygroscopicus in relation to conditions of continuous culture. J Gen Microbiol 128:107–114
Schüz TC (1990) Pelletbildung bei Streptomyces tendae Tü 901/S 2566 und verfahrenstechnische Optimierung der Nikkomycin-Fermentation. Dissertation, University of Tübingen
Schüz TC, Fiedler HP, Zähner H, Rieck M, König WA (1992) Metabolic products of microorganisms. 263. Nikkomycin S z ,S x ,So z and So x , new intermediates associated to the nikkomycin biosynthesis of Streptomyces tendae. J Antibiiot 45:199–206
Veelken M, Pape H (1984) Production of nikkomycin by immobilized Streptomyces cells — physiological properties. Appl Microbiol Biotechnol 19:146–152
Walz F (1986) Studien zur Fermentation neuer Nikkomycine mit freien und immobilisierten Zellen. Dissertation, University of Tübingen
Zoebelein G, Kniehase U (1985) Labor-, Gewächshaus- und Freilandversuche zur Wirkung von Nikkomycin gegen Insekten und Milben. Pflanzenschutznachr Bayer 38:203–304
Author information
Authors and Affiliations
Additional information
Correspondence to : T. Schüz
Rights and permissions
About this article
Cite this article
Schüz, T.C., Fiedler, HP. & Zähner, H. Optimized nikkomycin production by fed-batch and continuous fermentation. Appl Microbiol Biotechnol 39, 433–437 (1993). https://doi.org/10.1007/BF00205028
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00205028