Abstract
The frequency of ciliary beat was measured by laser light-scattering spectroscopy in cultures of ciliated cells of the rabbit oviduct. Measurements performed by this new method agree with those obtained by high speed cinematography. When beating cilia are illuminated by a laser beam, the scattered light shows a frequency modulation due to the oscillatory motion of cilia. The spectral structure of the scattered light depends on the frequency and time-space coherence of ciliary beat. This paper reports the experimental validation of this technique and the theoretical basis for obtaining the frequency and coherence of ciliary beat from the autocorrelation function of the spectrum of light scattered from moving cilia. Fiber optic light transmission could permit the extension of this method to assess ciliary activityin situ for applications in animal experimentation and clinical studies.
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Supported by USPHS Grant HL 20192-01 and Contract N01 HD 3-2788.
Supported by Fellowship HL 05147-18 from USPHS.
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Lee, W.I., Verdugo, P. Ciliary activity by laser light-scattering spectroscopy. Ann Biomed Eng 5, 248–259 (1977). https://doi.org/10.1007/BF02407872
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DOI: https://doi.org/10.1007/BF02407872