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Identification of amino acids involved in the binding of hMIP-1α to CC-CKR1, a MIP-lα receptor found on neutrophils

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Abstract

Human macrophage inflammatory protein-1α (hMIP-1α) and human macrophage inflammatory protein-1β (hMIP-1β) are chemokines involved in a diverse range of immunological effects. Both hMIP-1α and hMIP-1β are involved in the activation of monocytes and THP-1 cells probably through a common receptor(s). However, only hMIP-1α can bind to neutrophils with high affinity, presumably through CC-CKR1 (CKR1). Since the structure of these two proteins is highly conserved, non-conserved amino acids must define the disparate binding patterns that these two proteins exhibit. Measurements of binding, chemotaxis and calcium influx conducted with hMIP-1α and hMIP-1β chimeric proteins and mutants show that two amino acids (37K and 43L) are important in the binding and signaling of hMIP-1α through CKR1. Furthermore, we also show that mutations of the three charged amino acids at the C-terminus of hMIP-1α and hMIP-1β (amino acids 61, 65 and 67), do not adversely affect the binding to THP-1 cells.

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Authors and Affiliations

  1. Neurobiotechnology Center, The Ohio State University, Rightmire Hall, 1060 Carmack Road, Columbus, Ohio, USA

    Jacqueline M. Crisman & Pappachan E. Kolattukudy

  2. Arthur G. James Cancer Hospital and Research Institute, The Ohio State University, 300 West 10th Avenue, Columbus, Ohio, USA

    Jacqueline M. Crisman, Patrick J. Elder & Neil M. Wilkie

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  1. Jacqueline M. Crisman
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  2. Jacqueline M. Crisman
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  3. Patrick J. Elder
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Crisman, J.M., Crisman, J.M., Elder, P.J. et al. Identification of amino acids involved in the binding of hMIP-1α to CC-CKR1, a MIP-lα receptor found on neutrophils. Mol Cell Biochem 195, 245–256 (1999). https://doi.org/10.1023/A:1006901109902

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