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Effect of introduced Pseudomonas fluorescens strains on the uptake of nitrogen by wheat from 15N-enriched organic residues

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Abstract

The effects of an antibiotic-producing Pseudomonas fluorescens strain (F113) carrying the marker gene cassette lacZY and a marked, non-producing strain (F113G22) on the uptake of nitrogen from 15N-enriched organic residues incorporated into a sandy soil were investigated in microcosm studies. Strain F113 produces the antibiotic 2,4-diacetylphloroglucinol (DAPG), whilst its modified derivative strain F113G22 has DAPG production deleted by Tn5 mutagenesis. Uptake of nitrogen by wheat (Triticum aestivum) from 15N-enriched organic residues was estimated using stable isotope-ratio mass spectrometry of shoot and root material of 17-day-old plants. In addition, plant growth and active microbial biomass in soil were monitored. In contrast to results obtained in our previous study on pea (Pisum sativum), it was found that in wheat, inoculation with either strain F113 or F113G22 decreased the proportion of nitrogen derived from 15N-labelled organic residues incorporated into soil as compared to non-inoculated controls. It is therefore suggested that these strains decreased mineralization of organic residues in the rhizosphere of wheat, making less inorganic N (15N) available for plant uptake. The results of this study indicate that the effects of introduced Pseudomonas fluorescens strains on nitrogen mineralization in the rhizosphere are plant-species dependent, and highlight the importance of testing microbial inocula on a range of plant species.

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Authors and Affiliations

  1. School of Biological Sciences, University of Surrey, Guildford, Surrey, GU2 5XH, UK

    M.J. Brimecombe, F.A.A.M. De Leij & J.M. Lynch

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  1. M.J. Brimecombe
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  2. F.A.A.M. De Leij
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  3. J.M. Lynch
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Brimecombe, M., De Leij, F. & Lynch, J. Effect of introduced Pseudomonas fluorescens strains on the uptake of nitrogen by wheat from 15N-enriched organic residues. World Journal of Microbiology and Biotechnology 15, 417–423 (1999). https://doi.org/10.1023/A:1008999112121

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