Abstract
Microsporum gypseum strains obtained from human and animal cases of dermatophytosis were used to study the septal ontogeny, the germination, and the electron microscopy of the macroaleurioconidia, which are produced so abundantly by this organism.
It was found that the number of septa in a macroaleurioconidium depends upon the stage of development, and that their order of formation remains relatively constant. The macroaleurioconidial cell wall proved to be impressive on electron microscopy. The use of a wetting agent (Tween 80) and negative pressure proved necessary for adequate fixation. Poor penetration of the fixing agent is attributable to the electron-dense encrustations over the entire surface of the macroaleurioconidium.
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References
Blank H, Taplin D, Roth FJ: Electron microscopic observations of the effects of griseofulvin of the dermatophytes. AMA Arch Dermatol 81:69–82, 1960.
Delektorski WW et al.: Die Ultrastruktur des apikalen Segments der Hyphen mancher Dermatophyten. Mykosen 22:119–128, 1979.
Delektorsky VV, Sheklakov ND, Shadyev KhK: Ultrastructure of Microsporum canis in culture and in affected hair. Vestn Dermatol Venerol 6:10–13, 1975.
Findlay GH, Vismer HF (née Roux), Simson IW: Uncontrolled Microsporum gypseum infection in a newborn mammal. Br J Dermatol 85, Suppl 7:87–93, 1971.
Findlay GH, Vismer HF, Dreyer L: Studies on Sporotrichosis. Pathogenicity and morphogenesis in the Transvaal strains of Sporothrix schenckii. Mycopathologia 87:85–93, 1984.
Frey-Wyssling A, Mühlethaler K: Ultrastructural plant cytology with an introduction to molecular biology. Elsevier Publ Comp, Amsterdam, 377 pp, 1965.
Galgóczy J: Dermatophytes: Conidium-ontogeny and classification. Acta Microbiol Acad Sci Hung 22:105–136, 1975.
Ito Y et al.: An electron microscopic observation of Trichophyton violaceum. J Invest Dermatol 48:124–127, 1967.
Ito Y et al.: Further observations on the fine structure of Trichophyton violaceum: Non-pigmented, mutant mycelia. Sabouraudia 7:138–141, 1969.
Ito Y et al.: Surface structure of dermatophytes as seen by the scanning electron microscope. Sabouraudia 7:270–272, 1970.
Leighton TJ, Stock JJ: Heat-induced macroconidia germination in Microsporum gypseum. Appl Microbiol 17:473–475, 1969.
Leighton TJ, Stock JJ: Biochemical changes during fungal sporulation and spore germination. I. Phenyl methyl sulfonyl fluoride inhibition of macroconidial germination in Microsporum gypseum. J Bacteriol 101:931–940, 1970.
Meinhof W: Untersuchungen zur Ultrastruktur von Trichophyton verrucosum Bodin 1902 und Trichophyton schonleinii (Lebert) Langeron and Milochevitch 1930. Arch für klin u exper Dermat 229:265–275, 1967.
Meinhof W: Untersuchungen zur Ultrastruktur von Microsporum gypseum (Bodin 1907), Guiart et Grigoraki 1928. I. Aufbau der Zellwand der Septum und der cytoplasmatischen Membrane. Arch für klin u exper Dermat 228:111–121, 1967a.
Meinhof W: Untersuchungen zur Ultrastruktur von Microsporum gypseum (Bodin 1907), Guiart et Grigoraki 1928. II. Aufbau und Vermehrung der Mitochondrien. Arch für klin u exper Dermat 228:122–135, 1967b.
Moore RT: The ultrastructure of fungal cells. In: Ainsworth GC, Sussman AS (eds) The Fungi, Vol. 1. Academic Press, NY, London, 1965, pp 95–118.
Page WJ, Stock JJ: Regulation and self-inhibition of Microsporum gypseum macroconidia germination. J Bacteriol 108:276–281, 1971.
Page WJ, Stock JJ: Isolation and characterization of Microsporum gypseum lysosomes: Role of lysosomes in macroconidia germination. J Bact 110:354–362, 1972.
Pock-Steen B, Kobayasi T: Ultrastructure of the hyphal wall and septum of Trichophyton mentagrophytes. J Invest Dermatol 55:404–409, 1970.
Reichle RE, Alexander JV: Multiperforate septations, Woronin bodies and septal plugs in Fusarium. J Cell Biol 24:489–496, 1965.
Stoian M: Aspects ultrastructuraux des dermatophytes. Dermatologica 141:95–101, 1970.
Trinci AP: A kinetic study of the growth of Aspergillus nidulans and other fungi. J Gen Microbiol 57:11–24, 1969.
Tsukahara T, Sato A, Okada R: Electron microscopic studies on the cytological structure of Trichophyton mentagrophytes. Jpn J Microbiol 8:83–96, 1964.
Vismer HF, De Beer HA, Dreyer L: Subcutaneous phycomycosis caused by Basidiobolus haptosporus (Drechsler, 1947). S Afr Med J 58:644–647, 1980.
Visset MF: Les formes conidiennes du complexe Microsporum gypseum observees en microscopic electronique a balayage. Sabouraudia 10:191–192, 1972.
Visset MF, Vermeil C: Contribution à la connaissance des fungi keratinophiles de l'ouest de la France V. Etude au microscope electronique a balayage. Mycopath Mycol Appl 49:89–100, 1973.
Werner JH, Jolly HW Jr, Lee JH: Electron microscopic observations of Epidermophyton floccosum. J Invest Dermatol 43:139–143, 1964.
Werner JH, Jolly HW Jr, Spurlock BO: Electron microscopic observations of the fine structure of Microsporum canis. J Invest Dermatol 46:130–134, 1966.
Werner JH et al.: Electron microscopic observations of the fine structure of Microsporum gypseum. J Invest Dermatol 48:481–484, 1967.
Werner HJ, Jolly HW Jr, Carpenter CL Jr: Observations on the fine structure of Microsporum audouinii. J Invest Dermatol 50:276–279, 1968.
Zeidberg LD, Ajello L: Environmental factors influencing the occurrence of Histoplasma capsulatum and Microsporum gypseum in soil. J Bacteriol 68:156–159, 1954.
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This work forms part of a dissertation submitted by H.F.V. for the degree Magister Scientiae in the Department of Botany, University of Pretoria.
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Vismer, H.F., Findlay, G.H. & Eicker, A. The septal ontogeny, germination and electron microscopy of Microsporum gypseum macroaleurioconidia. Mycopathologia 98, 149–164 (1987). https://doi.org/10.1007/BF00437650
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DOI: https://doi.org/10.1007/BF00437650