Summary
Human sera containing the autoimmune specificities anti-RNP, anti-Sm or anti-La were used to screen a ν gt 11 human cDNA library. Positive clones were isolated and further characterized. To verify the specificity of the antibodies reacting with the cloned antigen, immobilized fusion proteins were used to select epitope specific antibodies. These were then used in immunofluorescence and immunoblotting experiments. The cDNA inserts from nositive clones were cloned into plasmid vectors to facilitate sequencing and mapping of the regions coding for the different autoantigenic epitopes.
References
Lerner MR and Steitz JA (1979). Antibodies to small nuclear RNAs complexed with proteins are produced by patients with systemic lupus erythematosus. P.N.A.S. 76:5495–5499.
Lerner EA et al. (1981). Monoclonal antibodies to nucleic acid containing cellular constituents: probes for molecular biology and autoimmune disease. P.N.A.S. 78:2737–2741. Tan E (1982). Autoantibodies to nuclear antigens (ANA): their immunobiology and medicine. Adv. Immunol. 33: 167–280.
Young A and Davis RW (1983). Yeast RNA polymerase II genes: Isolation with antibody probes, Science 222, 778–782.
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Pettersson, I., Nyman, U., Sharp, G.C. et al. Isolation of cDNA clones expressing the RNP, Sm and La autoantigens. Molecular Biology Reports 12, 156–157 (1987). https://doi.org/10.1007/BF00356874
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DOI: https://doi.org/10.1007/BF00356874