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Specific binding affinity for DNA of the L phage (Salmonella typhimurium) in extracts of Escherichia coli

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Abstract

The repressor gene c II of the L phage was cloned into plasmid pHC624 and expressed in E. coli. Two separate binding affinities for L phage DNA were identified during fractionation of protein extract of that strain. The activity that salts out in low concentration of ammonium sulphate belonged to the repressor, the activity that salts out in high concentrations of (NH4)2SO4 was proved to be of E. coli origin. Binding sites for the two proteins are located on different fragments of the L phage genome.

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References

  1. Bezděk, M. & Amati, P., 1967. Virology 31: 272–278.

    Google Scholar 

  2. Bezděk, M. & Amati, P., 1968. Virology 36: 701–703.

    Google Scholar 

  3. Boros, I., Póstfai, G. & Venetianer, P., 1984. Gene 30: 257–260.

    Google Scholar 

  4. DeAnda, J., Poteete, A.R. & Sauer, R.T., 1983. J. Biol. Chem. 258: 10536–10558.

    Google Scholar 

  5. Echols, H. & Green, L., 1971. Proc. Natl. Acad. Sci. U.S.A. 68: 2190–2194.

    Google Scholar 

  6. Geider, K. & Hoffman-Berling, H., 1981. Ann. Rev. Biochem. 50: 233–260.

    Google Scholar 

  7. Karlovský, P., Soška, J., Reich, J. & Kuhrová, V., 1984. FEMS Microbiol. Lett. 25: 117–120.

    Google Scholar 

  8. Laine, B. & Sautiere, P., 1984. Biochem. Biophys. Res. commun. 119: 1147–1153.

    Google Scholar 

  9. Lowry, O.H., Rosebrough, N.J., Farr, A.L. & Randall, R.J., 1951. J. Biol. Chem. 193: 263–275.

    Google Scholar 

  10. Maniatis, T., Fritsch, E.F. & Sambrook, J., 1982, In: Molecular Cloning, Cold Spring Harbor Laboratory Cold Spring Harbor, New York, pp. 97–173.

    Google Scholar 

  11. Miller, H.I., 1981. Cell 25: 269–276.

    Google Scholar 

  12. Miller, H.I., & Friedman, D.I., 1980. Cell 20: 711–719.

    Google Scholar 

  13. Miller, H.I. & Nash, H.A., 1981. Nature 290: 523–526.

    Google Scholar 

  14. Ptashne, M., Backman, K., Humayun, M.Z., Jeffrey, A., Maurer, R., Meyer, B. & Sauer, R.T., 1976. Science 194: 156–161.

    Google Scholar 

  15. Schleif, R.F. & Wensing, P.C., 1981. In: P. Mannor (ed.), Practical Methods in Molecular Biology, Springer-Verlag. New York, pp. 89–90.

    Google Scholar 

  16. Scopes, R., 1982. In: Ch.R. Cantor (ed.), Protein Purification. Springer-Verlag, New York, p. 262.

    Google Scholar 

  17. Susskind, M.M. & Botstein, D., 1978. Microbiol. Rev. 42: 385–413.

    Google Scholar 

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Authors and Affiliations

  1. Institute of Biophysics, Czechoslovak Academy of Sciences, Královopolská 135, 612 65, Brno, Czechoslovakia

    Petr Karlovský & Viera Kuhrová

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  1. Petr Karlovský
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  2. Viera Kuhrová
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Karlovský, P., Kuhrová, V. Specific binding affinity for DNA of the L phage (Salmonella typhimurium) in extracts of Escherichia coli. Mol Biol Rep 11, 43–46 (1986). https://doi.org/10.1007/BF00417594

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  • DOI: https://doi.org/10.1007/BF00417594

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