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Stimulation-dependent uptake of an extracellular marker to subcellular fractions of isolated neurohypophysial tissue

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Summary

Slices of ox neurohypophyses and groups of isolated rat neurointermediate lobes were incubated in a medium containing horseradish peroxidase (HRP) and stimulated by high K+ concentrations in the medium. After washing, the tissue was homogenized and subjected to subcellular fractionation in a Percoll/sucrose gradient. HRP was exclusively taken up by particles banding at a low density of the gradient. The HRP containing particles located to this region included vacuoles of a size comparable to secretory vesicles.

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Author notes

  1. M. Gratzl

    Present address: Abteilung Klinische Morphologie, Universität Ulm, Ulm, FRG

  2. J. T. Russell

    Present address: Section on Functional Neurochemistry, Nat. Inst. Child Health & Human Development, Bethesda, Md, USA

Authors and Affiliations

  1. Institute of Medical Physiology C, University of Copenhagen, The Panum Institute, Blegdamsvej 3c, DK-2200, Copenhagen N, Denmark

    M. Gratzl, J. T. Russell & N. A. Thorn

Authors
  1. M. Gratzl
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  2. J. T. Russell
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  3. N. A. Thorn
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Additional information

Acknowledgments. The authors thank Mrs. M. Elis and B. Lynderup for excellent technical assistance and help during preparation of the manuscript. This work was supported by the Deutsche Forschungsgemeinschaft (G. 681/2-1), the Danish Medical Research Council, the NOVO Foundation, the Nordisk Insulin Foundation and the P. Carl Petersen Foundation.

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Gratzl, M., Russell, J.T. & Thorn, N.A. Stimulation-dependent uptake of an extracellular marker to subcellular fractions of isolated neurohypophysial tissue. Experientia 39, 1007–1009 (1983). https://doi.org/10.1007/BF01989775

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  • DOI: https://doi.org/10.1007/BF01989775

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