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Addition of humic acid accelerates the growth of Euglena pisciformis AEW501 and the accumulation of lipids

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Abstract

As an excellent cell factory rich in polyunsaturated fatty acids, β-1,3-glucan, amino acids, and vitamins, Euglena has gained great attention with high-value products in recent years. In order to determine the optimal cultivation method of Euglena pisciformis AEW501, the effects of phototrophic, mixotrophic, and heterotrophic mode on its growth were investigated, and humic acid (HA) as a growth stimulant was added into the mixotrophic culture system to verify the hypothesis that HA addition could accelerate the growth and lipid accumulation of E. pisciformis AEW501. The results indicated that biomass of mixotrophic mode (330 ± 0.05 mg L−1) was 4.80 times and 7.65 times higher than that of phototrophic and heterotrophic mode, respectively, and mixotrophy was proved as the optimal trophic mode. Moreover. The maximum biomass productivity (34.63 mg L−1 day−1) and the highest lipid content (59.51%) were achieved in 50 mg L−1 HA treatment though there was no significant difference between 10 mg L−1 and 50 mg L−1 treatments. While photosynthetic efficiency of photosystem II, pigments content, cell size, and yield of eicosapentaenoic (EPA) and docosahexaenoic (DHA) in 10 mg L−1 HA treatment were the highest. It could be concluded that HA showed good promotion effects on growth and high-value products accumulation of E. pisciformis AEW501. This research will be helpful for the large-scale cultivation of E. pisciformis AEW501.

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Acknowledgements

This work was supported by the National Natural Science Foundation of China (No. 31971477). The authors thank the Analysis and Testing Center, institute of hydrobiology for the help in the measurement of EPA and DHA.

Funding

This work was supported by the National Natural Science Foundation of China (No:31971477).

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Authors and Affiliations

  1. State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, 430072, People’s Republic of China

    Panpan Fan, Pingping Xu, Yuxuan Zhu, Xiaojie Tu, Gaofei Song, Yanxia Zuo & Yonghong Bi

  2. University of Chinese Academy of Sciences, Beijing, 100049, People’s Republic of China

    Panpan Fan, Pingping Xu & Yuxuan Zhu

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  1. Panpan Fan
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  2. Pingping Xu
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Contributions

PF and YB conceived and designed the study. PF, PX, YZ, and YZ performed the experiments. PF performed experimental data processing, analysis, and writing of the manuscript. XT, GS, and YB reviewed and edited the manuscript.

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Correspondence to Yonghong Bi.

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Fan, P., Xu, P., Zhu, Y. et al. Addition of humic acid accelerates the growth of Euglena pisciformis AEW501 and the accumulation of lipids. J Appl Phycol 34, 51–63 (2022). https://doi.org/10.1007/s10811-021-02623-9

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