The 2023 MDPI Annual Report has
been released!
 
23 pages, 1237 KiB  
Review
Glass Fibre-Reinforced Composite Materials Used in the Aeronautical Transport Sector: A Critical Circular Economy Point of View
by George-Vаlentin Săftoiu, Cаrolinа Constantin , Аdriаn-Ionuț Nicoаră, George Pelin, Denisа Ficаi and Аnton Ficаi
Sustainability 2024, 16(11), 4632; https://doi.org/10.3390/su16114632 (registering DOI) - 29 May 2024
Abstract
Progress in composite materials has led to their use in applications where improved mechanical and resistance characteristics are required. Most composites are obtained in such a way that they present specific mechanical properties and/or have the role of both a thermal conductor and [...] Read more.
Progress in composite materials has led to their use in applications where improved mechanical and resistance characteristics are required. Most composites are obtained in such a way that they present specific mechanical properties and/or have the role of both a thermal conductor and insulator; these properties are important, specific, specialized, and useful. The advantages of these materials compared to the classic ones are as follows: low weight, high resistance to wear and corrosion, and mechanical characteristics consistent with the subsequent use of the product. The slightly high costs of these materials are justified by their precision, the quality of the products obtained, and the fact that their use leads to increased reliability, maintenance, and, in the cases of the automotive and aeronautical industries, reduced energy consumption. This paper aims to bring to readers’ attention the latest research related to glass fibre-reinforced composite materials in transport-related applications, such as automotive and aeronautic applications, including the manufacturing of unmanned aerial vehicles (UAVs). Considering the long period of use, the recycling and reuse of composite materials used in aeronautical transport is a must considering the environmental aspects and the need of achieving a circular economy. In recent years, considerable efforts have been made to find new alternatives to improve the performance and durability of materials in the aeronautical transport sector. Full article
19 pages, 614 KiB  
Article
On Block g-Circulant Matrices with Discrete Cosine and Sine Transforms for Transformer-Based Translation Machine
by Euis Asriani, Intan Muchtadi-Alamsyah and Ayu Purwarianti
Mathematics 2024, 12(11), 1697; https://doi.org/10.3390/math12111697 (registering DOI) - 29 May 2024
Abstract
Transformer has emerged as one of the modern neural networks that has been applied in numerous applications. However, transformers’ large and deep architecture makes them computationally and memory-intensive. In this paper, we propose the block g-circulant matrices to replace the dense weight [...] Read more.
Transformer has emerged as one of the modern neural networks that has been applied in numerous applications. However, transformers’ large and deep architecture makes them computationally and memory-intensive. In this paper, we propose the block g-circulant matrices to replace the dense weight matrices in the feedforward layers of the transformer and leverage the DCT-DST algorithm to multiply these matrices with the input vector. Our test using Portuguese-English datasets shows that the suggested method improves model memory efficiency compared to the dense transformer but at the cost of a slight drop in accuracy. We found that the model Dense-block 1-circulant DCT-DST of 128 dimensions achieved the highest model memory efficiency at 22.14%. We further show that the same model achieved a BLEU score of 26.47%. Full article
(This article belongs to the Special Issue Applications of Mathematics in Neural Networks and Machine Learning)
19 pages, 5543 KiB  
Article
Long-Term Changes in the Permafrost Temperature and Surface Frost Number in Northeast China
by Wei Shan, Lisha Qiu, Ying Guo, Chengcheng Zhang and Shuai Liu
Atmosphere 2024, 15(6), 652; https://doi.org/10.3390/atmos15060652 (registering DOI) - 29 May 2024
Abstract
The permafrost in Northeast China is experiencing rapid degradation due to the influence of climate change and human activities, profoundly impacting the local ecological environment and engineering construction. Understanding the spatiotemporal dynamics of long-term permafrost in this region is crucial; however, systematic research [...] Read more.
The permafrost in Northeast China is experiencing rapid degradation due to the influence of climate change and human activities, profoundly impacting the local ecological environment and engineering construction. Understanding the spatiotemporal dynamics of long-term permafrost in this region is crucial; however, systematic research on this topic remains scarce. This study combines meteorological station data, MODIS land surface temperature (LST) datasets, and borehole locations to apply the surface frost number (SFn) model. This approach enables the simulation and estimation of the spatial distribution and changes in the area of the surface frost number without vegetation effects (SFnv) and permafrost temperature (PT) in Northeast China from 1971 to 2020. The area of the SFnv > 0.49 within the permafrost region decreased substantially from approximately 44.353 × 104 km2 to 19.909 × 104 km2 between 1971 and 2020, with a notable change in 1988. The area of permafrost calculated using PT < 0 was slightly smaller, declining from 39.388 × 104 km2 to 29.852 × 104 km2. There was also a significant increase in the area with PT ranging from −1 °C to 0 °C, indicating a decline in permafrost stability. Approximately 10.926 × 104 km2 of stable permafrost has been transformed into semi-stable and unstable permafrost. Moreover, from 1982 to 2020, the NDVI was negatively correlated with the area of stable permafrost and positively correlated with the area of transitional or unstable permafrost. Vegetation cover decreased as transitional or unstable permafrost degraded. These findings provide valuable information for permafrost research and engineering development in cold regions, as well as for future planning and adaptation strategies. Full article
(This article belongs to the Section Biosphere/Hydrosphere/Land–Atmosphere Interactions)
19 pages, 3510 KiB  
Article
Biomass Allocation of China’s Forests as Indicated by a Literature-Based Allometry Database
by Yajie Hao, Zhongyi Sun and Zheng-Hong Tan
Forests 2024, 15(6), 942; https://doi.org/10.3390/f15060942 (registering DOI) - 29 May 2024
Abstract
Allometry reflects the quantitative relationship between the allocation of resources among different organs. Understanding patterns of forest biomass allocation is critical to comprehending global climate change and the response of terrestrial vegetation to climate change. By collecting and reorganizing the existing allometric models [...] Read more.
Allometry reflects the quantitative relationship between the allocation of resources among different organs. Understanding patterns of forest biomass allocation is critical to comprehending global climate change and the response of terrestrial vegetation to climate change. By collecting and reorganizing the existing allometric models of tree species in China, we established a database containing over 3000 empirical allometric models. Based on this database, we analyzed the model parameters and the effect of climate on forest biomass allocation under the context of ‘optimal allocation theory’. We showed that (1) the average and median exponent of power functions for above-ground biomass were 2.344 and 2.385, respectively, which significantly deviated from the theoretical prediction of 2.667 by metabolic theory (p < 0.01). (2) The parameters of the allometric model were not constant, and not significantly correlated with temperature, precipitation, latitude, and elevation (p > 0.05), but were more closely related to individual size (p < 0.01). (3) Among different types of forests, the proportion of above-ground biomass in tropical rainforests and subtropical evergreen rainforests was significantly higher than that in temperate forests and boreal forests (p < 0.05). The proportion of trunk and branch biomass allocated to tropical rainforest was significantly higher than that of boreal forest (p < 0.05), while the proportion of root and leaf biomass allocated to tropical rainforest was significantly lower than that of boreal forest (p < 0.05). (4) The abiotic environment plays a crucial role in determining the allocation of plant biomass. The ratio of below-ground/above-ground biomass is significantly and negatively correlated with both temperature and rainfall (p < 0.01), and significantly and positively correlated with altitude and latitude (p < 0.01). This means that as temperature and rainfall increase, there is a decrease in the amount of biomass allocated to below-ground structures such as roots. On the other hand, as altitude and latitude increase, there is an increase in below-ground biomass allocation. These findings highlight the importance of considering the influence of abiotic factors on plant growth and development. Full article
(This article belongs to the Section Forest Ecology and Management)
12 pages, 988 KiB  
Article
Exploring Variability: Inflammation Mediator Levels across Tissues and Time in Poultry Experimentally Infected by the G1a and G6 Genogroups of Infectious Bursal Disease Virus (IBDV)
by Giovanni Franzo, Giorgia Dotto, Caterina Lupini, Matteo Legnardi, Claudia Maria Tucciarone, Francesca Poletto, Elena Catelli, Giulia Graziosi, Mattia Cecchinato and Daniela Pasotto
Animals 2024, 14(11), 1619; https://doi.org/10.3390/ani14111619 (registering DOI) - 29 May 2024
Abstract
Infectious bursal disease virus (IBDV) is a significant burden for poultry production and market due to both direct disease and induced immunosuppression. In the present study, the expression of different cytokines in the bursa of Fabricius and thymus was evaluated during a 28-day-long [...] Read more.
Infectious bursal disease virus (IBDV) is a significant burden for poultry production and market due to both direct disease and induced immunosuppression. In the present study, the expression of different cytokines in the bursa of Fabricius and thymus was evaluated during a 28-day-long experimental infection with two strains classified in the G1a (Classical) and G6 (ITA) genogroups. Although both strains significantly affected and modulated the expression of different molecules, the G6 strain seemed to induce a delayed immune response or suppress it more promptly. A recovery in the expression of several mediators was observed in the G1a-infected group at the end of the study, but not in the G6 one, further supporting a more persistent immunosuppression. This evidence fits with the higher replication level previously reported for the G6 and with the clinical outcome, as this genotype, although subclinical, has often been considered more immunosuppressive. However, unlike other studies focused on shorter time periods after infection, the patterns observed in this paper were highly variable and complex, depending on the strain, tissue, and time point, and characterized by a non-negligible within-group variability. Besides confirming the strain/genogroup effect on immune system modulation, the present study suggests the usefulness of longer monitoring activities after experimental infection to better understand the complex patterns and interactions with the host response. Full article
31 pages, 2161 KiB  
Review
Insights into the Mechanisms of Action of Akkermansia muciniphila in the Treatment of Non-Communicable Diseases
by Honorata Mruk-Mazurkiewicz, Monika Kulaszyńska, Wiktoria Czarnecka, Albert Podkówka, Natalia Ekstedt, Piotr Zawodny, Anna Wierzbicka-Woś, Wojciech Marlicz, Błażej Skupin, Ewa Stachowska, Igor Łoniewski and Karolina Skonieczna-Żydecka
Nutrients 2024, 16(11), 1695; https://doi.org/10.3390/nu16111695 (registering DOI) - 29 May 2024
Abstract
This comprehensive review delineates the extensive roles of Akkermansia muciniphila in various health domains, spanning from metabolic and inflammatory diseases to neurodegenerative disorders. A. muciniphila, known for its ability to reside in the mucous layer of the intestine, plays a pivotal role [...] Read more.
This comprehensive review delineates the extensive roles of Akkermansia muciniphila in various health domains, spanning from metabolic and inflammatory diseases to neurodegenerative disorders. A. muciniphila, known for its ability to reside in the mucous layer of the intestine, plays a pivotal role in maintaining gut integrity and interacting with host metabolic processes. Its influence extends to modulating immune responses and potentially easing symptoms across several non-communicable diseases, including obesity, diabetes, inflammatory bowel disease, and cancer. Recent studies highlight its capacity to interact with the gut–brain axis, suggesting a possible impact on neuropsychiatric conditions. Despite the promising therapeutic potential of A. muciniphila highlighted in animal and preliminary human studies, challenges remain in its practical application due to stability and cultivation issues. However, the development of pasteurized forms and synthetic mediums offers new avenues for its use in clinical settings, as recognized by regulatory bodies like the European Food Safety Authority. This narrative review serves as a crucial resource for understanding the broad implications of A. muciniphila across different health conditions and its potential integration into therapeutic strategies. Full article
(This article belongs to the Section Prebiotics and Probiotics)
18 pages, 4289 KiB  
Article
Ginkgo biloba Sex Identification Methods Using Hyperspectral Imaging and Machine Learning
by Mengyuan Chen, Chenfeng Lin, Yongqi Sun, Rui Yang, Xiangyu Lu, Weidong Lou, Xunfei Deng, Yunpeng Zhao and Fei Liu
Plants 2024, 13(11), 1501; https://doi.org/10.3390/plants13111501 (registering DOI) - 29 May 2024
Abstract
Ginkgo biloba L. is a rare dioecious species that is valued for its diverse applications and is cultivated globally. This study aimed to develop a rapid and effective method for determining the sex of a Ginkgo biloba. Green and yellow leaves representing [...] Read more.
Ginkgo biloba L. is a rare dioecious species that is valued for its diverse applications and is cultivated globally. This study aimed to develop a rapid and effective method for determining the sex of a Ginkgo biloba. Green and yellow leaves representing annual growth stages were scanned with a hyperspectral imager, and classification models for RGB images, spectral features, and a fusion of spectral and image features were established. Initially, a ResNet101 model classified the RGB dataset using the proportional scaling–background expansion preprocessing method, achieving an accuracy of 90.27%. Further, machine learning algorithms like support vector machine (SVM), linear discriminant analysis (LDA), and subspace discriminant analysis (SDA) were applied. Optimal results were achieved with SVM and SDA in the green leaf stage and LDA in the yellow leaf stage, with prediction accuracies of 87.35% and 98.85%, respectively. To fully utilize the optimal model, a two-stage Period-Predetermined (PP) method was proposed, and a fusion dataset was built using the spectral and image features. The overall accuracy for the prediction set was as high as 96.30%. This is the first study to establish a standard technique framework for Ginkgo sex classification using hyperspectral imaging, offering an efficient tool for industrial and ecological applications and the potential for classifying other dioecious plants. Full article
22 pages, 2663 KiB  
Article
Developing Different Test Conditions to Verify the Robustness and Versatility of Robotic Arms Controlled by Evolutionary Algorithms
by Roland Szabo
Electronics 2024, 13(11), 2130; https://doi.org/10.3390/electronics13112130 (registering DOI) - 29 May 2024
Abstract
In this paper, different test cases where robotic arms are tested will be presented. A robotic arm is tested for the gravity effects that can be observed on it. The other robotic arm is tested for how much precision it has by using [...] Read more.
In this paper, different test cases where robotic arms are tested will be presented. A robotic arm is tested for the gravity effects that can be observed on it. The other robotic arm is tested for how much precision it has by using it to learn to write. The other robotic arm is tested on how well it can function as a solar tracker and how precisely it can function as an energy harvester. On the basis of these tests, the robotic arm’s mechanical structure, electronics, and software are put to the test. The software is based on evolutionary software that implements genetic algorithms. The entire command system is also ported to FPGAs (to hardware) to increase speed and response time. Full article
(This article belongs to the Section Industrial Electronics)
20 pages, 2494 KiB  
Review
Cell Migration Assays and Their Application to Wound Healing Assays—A Critical Review
by Chun Yang, Di Yin, Hongbo Zhang, Ildiko Badea, Shih-Mo Yang and Wenjun Zhang
Micromachines 2024, 15(6), 720; https://doi.org/10.3390/mi15060720 (registering DOI) - 29 May 2024
Abstract
In recent years, cell migration assays (CMAs) have emerged as a tool to study the migration of cells along with their physiological responses under various stimuli, including both mechanical and bio-chemical properties. CMAs are a generic system in that they support various biological [...] Read more.
In recent years, cell migration assays (CMAs) have emerged as a tool to study the migration of cells along with their physiological responses under various stimuli, including both mechanical and bio-chemical properties. CMAs are a generic system in that they support various biological applications, such as wound healing assays. In this paper, we review the development of the CMA in the context of its application to wound healing assays. As such, the wound healing assay will be used to derive the requirements on CMAs. This paper will provide a comprehensive and critical review of the development of CMAs along with their application to wound healing assays. One salient feature of our methodology in this paper is the application of the so-called design thinking; namely we define the requirements of CMAs first and then take them as a benchmark for various developments of CMAs in the literature. The state-of-the-art CMAs are compared with this benchmark to derive the knowledge and technological gap with CMAs in the literature. We will also discuss future research directions for the CMA together with its application to wound healing assays. Full article
(This article belongs to the Special Issue Research Progress of Microfluidic Bioseparation and Bioassay)
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16 pages, 902 KiB  
Article
Synthesis and Photovoltaic Performance of β-Amino-Substituted Porphyrin Derivatives
by Ana F. R. Cerqueira, Ana Lucia Pinto, Gabriela Malta, Maria G. P. M. S. Neves, A. Jorge Parola and Augusto C. Tomé
Int. J. Mol. Sci. 2024, 25(11), 5979; https://doi.org/10.3390/ijms25115979 (registering DOI) - 29 May 2024
Abstract
New β-amino-substituted porphyrin derivatives bearing carboxy groups were synthesized and their performance as sensitizers in dye-sensitized solar cells (DSSC) was evaluated. The new compounds were obtained in good yields (63–74%) through nucleophilic aromatic substitution reactions with 3-sulfanyl- and 4-sulfanylbenzoic acids. Although the electrochemical [...] Read more.
New β-amino-substituted porphyrin derivatives bearing carboxy groups were synthesized and their performance as sensitizers in dye-sensitized solar cells (DSSC) was evaluated. The new compounds were obtained in good yields (63–74%) through nucleophilic aromatic substitution reactions with 3-sulfanyl- and 4-sulfanylbenzoic acids. Although the electrochemical studies indicated suitable HOMO and LUMO energy levels for use in DSSC, the devices fabricated with these compounds revealed a low power conversion efficiency (PCE) that is primarily due to the low open-circuit voltage (Voc) and short-circuit current density (Jsc) values. Full article
18 pages, 5368 KiB  
Article
Evaluation of Calcarenite Degradation by X-ray Photoelectron Spectroscopy Analysis inside the Rupestrian Church of San Pietro Barisano (Matera, Southern Italy)
by Francesco Cardellicchio, Maria Assunta Acquavia, Mariangela Curcio and Anna Maria Salvi
Sustainability 2024, 16(11), 4634; https://doi.org/10.3390/su16114634 (registering DOI) - 29 May 2024
Abstract
We report on the XPS analysis of degraded surfaces inside San Pietro Barisano, the rupestrian church carved into the calcarenite rock of ancient Matera, which has been a UNESCO World Heritage Site since 1993. As reported in previous works, the “Sassi” district and [...] Read more.
We report on the XPS analysis of degraded surfaces inside San Pietro Barisano, the rupestrian church carved into the calcarenite rock of ancient Matera, which has been a UNESCO World Heritage Site since 1993. As reported in previous works, the “Sassi” district and the park of rupestrian churches were available as open laboratories for the National Smart Cities SCN_00520 research project dedicated to the sustainable recovery of this remarkable architectural heritage. In that context, XPS functionality was shown to reside in the possibility of analyzing surfaces by feasible sampling, acquiring spectra without any preliminary sample treatment, and processing data using a well-established curve fitting procedure. The obtained results allowed us to identify the degradation products of the investigated surfaces, thus contributing to defining a diagnostic framework for subsequent actions. Accordingly, the samples here considered, collected from the internal wall surfaces of the church, were all analyzed in comparison with the reference calcarenite, and the XPS results were evaluated as a function of local environmental factors and the historical context of the church itself. The final aim was to provide, for each sample, the most representative indicator(s) of biotic and/or abiotic degradation for reliable use, in a multidisciplinary context, in planning care interventions for building heritage. Full article
(This article belongs to the Special Issue Sustainable Conservation of Urban and Cultural Heritage)
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8 pages, 490 KiB  
Commentary
The Case for Pulmonary Metastasectomy—Clinical Practice Narrative Review and Commentary
by Paolo Scanagatta, Gianluca Ancona, Sara Cagnetti, Casimiro Eugenio Giorgetta, Francesco Inzirillo, Eugenio Ravalli, Martina Maiolani and Giuseppe Naldi
Life 2024, 14(6), 702; https://doi.org/10.3390/life14060702 (registering DOI) - 29 May 2024
Abstract
Pulmonary metastasectomy has become a well-established procedure for patients with certain types of solid tumors. Patients are usually scheduled for staged lung metastasectomy in case of primary tumor control, the absence of distant non-lung metastases, and when complete resection is achievable. Nodules are [...] Read more.
Pulmonary metastasectomy has become a well-established procedure for patients with certain types of solid tumors. Patients are usually scheduled for staged lung metastasectomy in case of primary tumor control, the absence of distant non-lung metastases, and when complete resection is achievable. Nodules are removed with precision resection in order to ensure radical resection with minimal margins; this technique permits good oncological results, preserving the surrounding pulmonary parenchyma and causing minimal distortion compared to staplers. When possible, anatomical resections should be avoided since they are not justified by real oncological advantages and, in the majority of cases, sacrifice too much healthy tissue, possibly leading to inoperability in the case of metachronous relapses. Thus, preserving the maximum amount of pulmonary parenchyma is crucial because repeated metastasectomies are possible and frequent, with no theoretical limits to the number of reinterventions. In our multidisciplinary board team, we support the role of pulmonary metastasectomy as a useful curative therapy, with acceptable morbidity and mortality, with indications to be discussed case-by-case. Full article
(This article belongs to the Special Issue Recent Advances in Modern Thoracic Surgery)
12 pages, 902 KiB  
Article
Evaluation of Changes in Activities of Daily Living and Quality of Life of Patients with Bone Metastasis Who Underwent Conservative Therapy through Bone Metastasis Cancer Boards
by Yasumitsu Fujii, Ryo Yoshikawa, Ryoga Kashima, Wataru Saho, Hirokazu Onishi, Tsuyoshi Matsumoto, Risa Harada, Yoshiki Takeoka, Ryoko Sawada, Naomasa Fukase, Hitomi Hara, Kenichiro Kakutani, Toshihiro Akisue and Yoshitada Sakai
Medicina 2024, 60(6), 906; https://doi.org/10.3390/medicina60060906 (registering DOI) - 29 May 2024
Abstract
Background and Objectives: Changes in activities of daily living (ADL) and quality of life (QOL) of patients with bone metastasis who underwent surgical treatment through Bone Metastasis Cancer Boards (BMCBs), a recent multidisciplinary approach for managing bone metastases, have been reported; however, [...] Read more.
Background and Objectives: Changes in activities of daily living (ADL) and quality of life (QOL) of patients with bone metastasis who underwent surgical treatment through Bone Metastasis Cancer Boards (BMCBs), a recent multidisciplinary approach for managing bone metastases, have been reported; however, no reports exist on patients who undergo conservative treatment. In this study, we aimed to evaluate these patients’ ADL and QOL and examine the factors influencing changes in these parameters. Materials and Methods: We retrospectively reviewed 200 patients with bone metastases who underwent conservative therapy through BMCBs between 2013 and 2021. A reassessment was conducted within 2–8 weeks after the initial assessment. Patients’ background and changes in performance status (PS), Barthel Index (BI), EuroQol five-dimension (EQ-5D) scores, and Numerical Rating Scale (NRS) scores were initially assessed. Furthermore, we categorized patients into two groups based on improvements or deteriorations in ADL and QOL and performed comparative analyses. Results: Significant improvements in EQ-5D (0.57 ± 0.02 versus [vs.] 0.64 ± 0.02), NRS max (5.21 ± 0.24 vs. 3.56 ± 0.21), and NRS average (2.98 ± 0.18 vs. 1.85 ± 0.13) scores were observed between the initial assessment and reassessment (all p < 0.001). PS (1.84 ± 0.08 vs. 1.72 ± 0.08) and BI (83.15 ± 1.68 vs. 84.42 ± 1.73) also showed improvements (p = 0.06, and 0.054, respectively). In addition, spinal cord paralysis (odds ratio [OR]: 3.69, p = 0.049; OR: 8.42, p < 0.001), chemotherapy (OR: 0.43, p = 0.02; OR: 0.25, p = 0.007), and NRS average scores (OR: 0.38, p = 0.02; OR: 0.14, p < 0.001) were independent factors associated with ADL and QOL. Conclusions: Patients with bone metastases who underwent conservative treatment through BMCBs exhibited an increase in QOL without a decline in ADL. The presence of spinal cord paralysis, absence of chemotherapy, and poor pain control were associated with a higher risk of deterioration in ADL and QOL. Full article
(This article belongs to the Section Orthopedics)
16 pages, 338 KiB  
Article
Rapid Beam Tracking Using Power Measurement for Terahertz Communications
by Xiaodan He, Changming Zhang, Chi Lu and Xianbin Yu
Electronics 2024, 13(11), 2129; https://doi.org/10.3390/electronics13112129 (registering DOI) - 29 May 2024
Abstract
With abundant bandwidth resources, terahertz communications are considered one of the key technologies to meet the requirement for high data-rate transmission in the future. In order to compensate for the severe propagation loss of terahertz communications, directional antennas with high gain and narrow [...] Read more.
With abundant bandwidth resources, terahertz communications are considered one of the key technologies to meet the requirement for high data-rate transmission in the future. In order to compensate for the severe propagation loss of terahertz communications, directional antennas with high gain and narrow beams are expected to be adopted, making beam tracking significant for robust communications. In this paper, a tracking method based on power measurement is proposed, consisting of beam status monitoring, recognition of the deviation direction, and movement toward the optimal angle. By observing the change in the received signal power, beam misalignment is first checked, and whether the misalignment is out of tracking range is also determined. Then, the deviation direction is recognized by comparing the received power variations in the candidate directions, and the beam angle is adjusted accordingly until it reaches the optimal angle. With a small scanning range, the deviation direction is recognized in a short duration, allowing for rapid beam tracking. Numerical results indicate that the alignment error is competitively low and stable in the proposed beam tracking method, and its technical superiority is particularly dominant in situations involving variable motion at high speeds. Full article
20 pages, 1658 KiB  
Article
Disrupting EGFR–HER2 Transactivation by Pertuzumab in HER2-Positive Cancer: Quantitative Analysis Reveals EGFR Signal Input as Potential Predictor of Therapeutic Outcome
by László Ujlaky-Nagy, János Szöllősi and György Vereb
Int. J. Mol. Sci. 2024, 25(11), 5978; https://doi.org/10.3390/ijms25115978 (registering DOI) - 29 May 2024
Abstract
Pertuzumab (Perjeta®), a humanized antibody binding to the dimerization arm of HER2 (Human epidermal growth factor receptor-2), has failed as a monotherapy agent in HER2 overexpressing malignancies. Since the molecular interaction of HER2 with ligand-bound EGFR (epidermal growth factor receptor) has [...] Read more.
Pertuzumab (Perjeta®), a humanized antibody binding to the dimerization arm of HER2 (Human epidermal growth factor receptor-2), has failed as a monotherapy agent in HER2 overexpressing malignancies. Since the molecular interaction of HER2 with ligand-bound EGFR (epidermal growth factor receptor) has been implied in mitogenic signaling and malignant proliferation, we hypothesized that this interaction, rather than HER2 expression and oligomerization alone, could be a potential molecular target and predictor of the efficacy of pertuzumab treatment. Therefore, we investigated static and dynamic interactions between HER2 and EGFR molecules upon EGF stimulus in the presence and absence of pertuzumab in HER2+ EGFR+ SK-BR-3 breast tumor cells using Förster resonance energy transfer (FRET) microscopy and fluorescence correlation and cross-correlation spectroscopy (FCS/FCCS). The consequential activation of signaling and changes in cell proliferation were measured by Western blotting and MTT assay. The autocorrelation functions of HER2 diffusion were best fitted by a three-component model corrected for triplet formation, and among these components the slowly diffusing membrane component revealed aggregation induced by EGFR ligand binding, as evidenced by photon-counting histograms and co-diffusing fractions. This aggregation has efficiently been prevented by pertuzumab treatment, which also inhibited the post-stimulus interaction of EGFR and HER2, as monitored by changes in FRET efficiency. Overall, the data demonstrated that pertuzumab, by hindering post-stimulus interaction between EGFR and HER2, inhibits EGFR-evoked HER2 aggregation and phosphorylation and leads to a dose-dependent decrease in cell proliferation, particularly when higher amounts of EGF are present. Consequently, we propose that EGFR expression on HER2-positive tumors could be taken into consideration as a potential biomarker when predicting the outcome of pertuzumab treatment. Full article
17 pages, 1777 KiB  
Article
Sustainable Treatment of Swine Wastewater: Optimizing the Culture Conditions of Tetradesmus cf. obliquus to Improve Treatment Efficiency
by Kailong Bai, Wenying Qu, Duo Song, Junfeng Li and Shih-Hsin Ho
Sustainability 2024, 16(11), 4633; https://doi.org/10.3390/su16114633 (registering DOI) - 29 May 2024
Abstract
To enhance the sustainability of microalgae-based swine wastewater treatment, this study aims to address the challenges of low efficiency in treating raw swine wastewater, collection difficulties, and high energy consumption treatment processes. The microalgae with strong environmental tolerance were first screened from swine [...] Read more.
To enhance the sustainability of microalgae-based swine wastewater treatment, this study aims to address the challenges of low efficiency in treating raw swine wastewater, collection difficulties, and high energy consumption treatment processes. The microalgae with strong environmental tolerance were first screened from swine wastewater, and its cultivation conditions were optimized to examine the effect of microalgae treatment on swine wastewater under optimal cultivation conditions. Additionally, the flocculation efficiency and mechanism of microalgae were analyzed. The results showed that Tetradesmus cf. obliquus ZYY1 exhibited the most robust heterotrophic growth. In the BG11 medium supplemented with glucose, the growth rate of T. cf. obliquus ZYY1 under chemoheterotrophic conditions was superior to its growth under photoheterotrophic conditions, reaching its peak with an optimal glucose concentration of 15 g/L. The biomass concentration of T. cf. obliquus ZYY1 in raw wastewater was significantly higher than that in sterilized wastewater, which reached 1.65 ± 0.01 g/L on the 10th day of treatment, with removal efficiencies of -N, -P, and the chemical oxygen demand reached 71.36%, 96.09%, and 93.13%, respectively. After raw wastewater treatment, the flocculation efficiency of T. cf. obliquus ZYY1 reached 97.71 ± 5.81%. This was attributed to the bacteria present in the raw wastewater, which induced T. cf. obliquus ZYY1 to secrete aromatic proteins. This study emphasizes the potential of microalgae as a green technology for sustainable wastewater treatment, offering a practical pathway for environmental protection and resource conservation. Full article
24 pages, 2564 KiB  
Article
Adaptive Quasi-Super-Twisting Sliding Mode Control for Flexible Multistate Switch
by Wenzhong Ma, Xiao Wang, Yusheng Wang, Wenyan Zhang, Hengshuo Li and Yaheng Zhu
Energies 2024, 17(11), 2643; https://doi.org/10.3390/en17112643 (registering DOI) - 29 May 2024
Abstract
The mathematical model of a flexible multistate switch (FMSS) exhibits nonlinear and strong coupling characteristics, whereas traditional power decoupling control makes it difficult to completely decouple the output power. The traditional proportional–integral control parameters are difficult to adjust, and their robustness and dynamic [...] Read more.
The mathematical model of a flexible multistate switch (FMSS) exhibits nonlinear and strong coupling characteristics, whereas traditional power decoupling control makes it difficult to completely decouple the output power. The traditional proportional–integral control parameters are difficult to adjust, and their robustness and dynamic performance are poor, which affects the stability of the voltage of the power distribution network and feeder power. To address these problems, this study first converted the original system into a linear system via coordinate transformation using feedback-accurate linearization to decouple active and reactive currents. Thereafter, a super-twisting sliding mode control (ST-SMC) algorithm was introduced, and an adaptive quasi-super-twisting sliding mode control (AQST-SMC) algorithm comprising the quasi-sliding mode function and adaptive proportional term was proposed. An FMSS double closed-loop controller was designed to achieve improved vibration suppression and convergence speed. A three-port FMSS simulation model was developed using MATLAB/Simulink, and the simulation results show that the proposed control strategy enhances the robustness and dynamic performance of the system. Full article
(This article belongs to the Special Issue Advanced Power Electronics Technology)
25 pages, 1165 KiB  
Article
Multimodal Mass Spectrometry Imaging of an Osteosarcoma Multicellular Tumour Spheroid Model to Investigate Drug-Induced Response
by Sophie M. Pearce, Neil A. Cross, David P. Smith, Malcolm R. Clench, Lucy E. Flint, Gregory Hamm, Richard Goodwin, James I. Langridge, Emmanuelle Claude and Laura M. Cole
Metabolites 2024, 14(6), 315; https://doi.org/10.3390/metabo14060315 (registering DOI) - 29 May 2024
Abstract
A multimodal mass spectrometry imaging (MSI) approach was used to investigate the chemotherapy drug-induced response of a Multicellular Tumour Spheroid (MCTS) 3D cell culture model of osteosarcoma (OS). The work addresses the critical demand for enhanced translatable early drug discovery approaches by demonstrating [...] Read more.
A multimodal mass spectrometry imaging (MSI) approach was used to investigate the chemotherapy drug-induced response of a Multicellular Tumour Spheroid (MCTS) 3D cell culture model of osteosarcoma (OS). The work addresses the critical demand for enhanced translatable early drug discovery approaches by demonstrating a robust spatially resolved molecular distribution analysis in tumour models following chemotherapeutic intervention. Advanced high-resolution techniques were employed, including desorption electrospray ionisation (DESI) mass spectrometry imaging (MSI), to assess the interplay between metabolic and cellular pathways in response to chemotherapeutic intervention. Endogenous metabolite distributions of the human OS tumour models were complemented with subcellularly resolved protein localisation by the detection of metal-tagged antibodies using Imaging Mass Cytometry (IMC). The first application of matrix-assisted laser desorption ionization–immunohistochemistry (MALDI-IHC) of 3D cell culture models is reported here. Protein localisation and expression following an acute dosage of the chemotherapy drug doxorubicin demonstrated novel indications for mechanisms of region-specific tumour survival and cell-cycle-specific drug-induced responses. Previously unknown doxorubicin-induced metabolite upregulation was revealed by DESI-MSI of MCTSs, which may be used to inform mechanisms of chemotherapeutic resistance. The demonstration of specific tumour survival mechanisms that are characteristic of those reported for in vivo tumours has underscored the increasing value of this approach as a tool to investigate drug resistance. Full article
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21 pages, 1088 KiB  
Review
The Antifungal Potential of Niclosamide and Structurally Related Salicylanilides
by Bernhard Biersack
Int. J. Mol. Sci. 2024, 25(11), 5977; https://doi.org/10.3390/ijms25115977 (registering DOI) - 29 May 2024
Abstract
Human mycoses cover a diverse field of fungal diseases from skin disorders to systemic invasive infections and pose an increasing global health problem based on ineffective treatment options, the hampered development of new efficient drugs, and the emergence of resistant fungal strains. Niclosamide [...] Read more.
Human mycoses cover a diverse field of fungal diseases from skin disorders to systemic invasive infections and pose an increasing global health problem based on ineffective treatment options, the hampered development of new efficient drugs, and the emergence of resistant fungal strains. Niclosamide is currently applied for the treatment of worm infections. Its mechanisms of action, which include the suppression of mitochondrial oxidative phosphorylation (also known as mitochondrial uncoupling), among others, has led to a repurposing of this promising anthelmintic drug for the therapy of further human diseases such as cancer, diabetes, and microbial infections. Given the urgent need to develop new drugs against fungal infections, the considerable antifungal properties of niclosamide are highlighted in this review. Its chemical and pharmacological properties relevant for drug development are also briefly mentioned, and the described mitochondria-targeting mechanisms of action add to the current arsenal of approved antifungal drugs. In addition, the activities of further salicylanilide-based niclosamide analogs against fungal pathogens, including agents applied in veterinary medicine for many years, are described and discussed for their feasibility as new antifungals for humans. Preliminary structure–activity relationships are determined and discussed. Various salicylanilide derivatives with antifungal activities showed increased oral bioavailabilities when compared with niclosamide. The simple synthesis of salicylanilide-based drugs also vouchsafes a broad and cost-effective availability for poorer patient groups. Pertinent literature is covered until 2024. Full article
(This article belongs to the Special Issue Antifungal Drug Discovery: Progresses, Challenges, Opportunities)
14 pages, 725 KiB  
Article
Association of Prenatal Dietary Toxicants and Inorganic Arsenic Exposure with Children’s Emotional and Behavioral Problems: ECLIPSES Study
by Xiruo Kou, Josefa Canals, Monica Bulló, Nerea Becerra-Tomás, Cristina Jardí and Victoria Arija
Toxics 2024, 12(6), 398; https://doi.org/10.3390/toxics12060398 (registering DOI) - 29 May 2024
Abstract
Prenatal exposure to dietary toxicants is linked to neurocognitive issues, but its effect on early emotional and behavioral development in children is less clear. To explore the relationship between prenatal intake of As, iAs, Cd, MeHg, Pb, PCDD/Fs, DL-PCBs, and NDL-PCBs and emotional [...] Read more.
Prenatal exposure to dietary toxicants is linked to neurocognitive issues, but its effect on early emotional and behavioral development in children is less clear. To explore the relationship between prenatal intake of As, iAs, Cd, MeHg, Pb, PCDD/Fs, DL-PCBs, and NDL-PCBs and emotional and behavioral issues in four-year-old children. This study included 192 mother–child pairs from the ECLIPSES study, assessing prenatal dietary toxicant exposure through a food-frequency questionnaire and Catalan Food Safety Agency data. Children’s emotional and behavioral scores were evaluated using the Child Behavior Checklist for ages 1.5–5 years. Multivariable regression and logistic models were used, focusing on iAs after finding significant preliminary associations. Increased prenatal dietary intake of iAs was associated with internalizing, externalizing, and attention-deficit/hyperactivity problems. Higher iAs levels (>4.16 μg/day) significantly increased the risk of total problems (OR = 2.94) and specific issues like anxious/depressed (OR = 4.88), anxiety (OR = 3.27), and oppositional defiant problems (OR = 4.30). High iAs consumption correlated with the intake of meat, eggs, cereals, tubers, fruits, and pulses Prenatal dietary iAs exposure is associated with various emotional and behavioral problems in children. Monitoring and reducing iAs levels in food are crucial for public health. Full article
(This article belongs to the Special Issue Toxicology Research of Foodborne Contaminants)
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13 pages, 786 KiB  
Review
Thymic Stromal Lymphopoietin and Tezepelumab in Airway Diseases: From Physiological Role to Target Therapy
by Diego Bagnasco, Laura De Ferrari, Benedetta Bondi, Maria Giulia Candeliere, Marcello Mincarini, Anna Maria Riccio and Fulvio Braido
Int. J. Mol. Sci. 2024, 25(11), 5972; https://doi.org/10.3390/ijms25115972 (registering DOI) - 29 May 2024
Abstract
Thymic stromal lymphopoietin (TSLP), is a protein belonging to a class of epithelial cytokines commonly called alarmins, which also includes IL-25 and IL-33. Functionally, TSLP is a key player in the immune response to environmental insults, initiating a number of downstream inflammatory pathways. [...] Read more.
Thymic stromal lymphopoietin (TSLP), is a protein belonging to a class of epithelial cytokines commonly called alarmins, which also includes IL-25 and IL-33. Functionally, TSLP is a key player in the immune response to environmental insults, initiating a number of downstream inflammatory pathways. TSLP performs its role by binding to a high-affinity heteromeric complex composed of the thymic stromal lymphopoietin receptor (TSLPR) chain and IL-7Rα. In recent years, the important role of proinflammatory cytokines in the etiopathogenesis of various chronic diseases such as asthma, chronic rhinosinusitis with nasal polyposis (CRSwNP), chronic obstructive pulmonary diseases (COPDs), and chronic spontaneous urticaria has been studied. Although alarmins have been found to be mainly implicated in the mechanisms of type 2 inflammation, studies on monoclonal antibodies against TSLP demonstrate partial efficacy even in patients whose inflammation is not definable as T2 and the so-called low T2. Tezepelumab is a human anti-TSLP antibody that prevents TSLP-TSLPR interactions. Several clinical trials are evaluating the safety and efficacy of Tezepelumab in various inflammatory disorders. In this review, we will highlight major recent advances in understanding the functional role of TSLP, its involvement in Th2-related diseases, and its suitability as a target for biological therapies. Full article
(This article belongs to the Special Issue Molecular Advance on the Pathogenesis and Treatment of Asthma)
13 pages, 2839 KiB  
Article
Annual and Seasonal Dynamics of CO2 Emissions in Major Cities of China (2019–2022)
by Yue Zhao, Yuning Feng, Mingyi Du and Klaus Fraedrich
ISPRS Int. J. Geo-Inf. 2024, 13(6), 181; https://doi.org/10.3390/ijgi13060181 (registering DOI) - 29 May 2024
Abstract
To control the growth of CO2 emissions and achieve the goal of carbon peaking, this study carried out a detailed spatio-temporal analysis of carbon emissions in major cities of China on a city-wide and seasonal scale, used carbon emissions as an indicator [...] Read more.
To control the growth of CO2 emissions and achieve the goal of carbon peaking, this study carried out a detailed spatio-temporal analysis of carbon emissions in major cities of China on a city-wide and seasonal scale, used carbon emissions as an indicator to explore the impact of COVID-19 on human activities, and thereby studied the urban resilience of different cities. Our research re-vealed that (i) the seasonal patterns of CO2 emissions in major cities of China could be divided into four types: Long High, Summer High, Winter High, and Fluctuations, which was highly related to the power and industrial sectors. (ii) The annual trends, which were strongly affected by the pan-demic, could be divided into four types: Little Impact, First Impact, Second Impact, and Both Impact. (iii) The recovery speed of CO2 emissions reflected urban resilience. Cities with higher levels of de-velopment had a stronger resistance to the pandemic, but a slower recovery speed. Studying the changes in CO2 emissions and their causes can help to make timely policy adjustments during the economic recovery period after the end of the pandemic, provide more references to urban resilience construction, and provide experience for future responses to large-scale emergencies. Full article
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21 pages, 2442 KiB  
Article
Acoustic Pressure Amplification through In-Duct Sonic Black Holes
by Cédric Maury, Teresa Bravo, Muriel Amielh and Daniel Mazzoni
Appl. Sci. 2024, 14(11), 4699; https://doi.org/10.3390/app14114699 (registering DOI) - 29 May 2024
Abstract
Acoustic detection of machinery defaults from in-duct measurements is of practical importance in many areas, such as the health assessment of turbines in ventilation systems or engine testing in the surface and air transport sectors. This approach is, however, impeded by the low [...] Read more.
Acoustic detection of machinery defaults from in-duct measurements is of practical importance in many areas, such as the health assessment of turbines in ventilation systems or engine testing in the surface and air transport sectors. This approach is, however, impeded by the low signal-to-noise ratio (SNR) observed in such environments. In this study, it is proposed to exploit the slow sound effect of Sonic Black Hole (SBH) ducted silencers to enhance the sensing of incident pulse acoustic signals with low SNR. It is found from transfer matrix and finite element modelling that fully opened SBH silencers with perforated skin interfaces are able to substantially enhance an incident pulse amplitude while channeling an air flow. We demonstrate that the graded depths of the SBH cavities provide rainbow spectral decomposition and amplification of the incident pulse frequency components, provided that impedance matching, slow sound, and critically coupled conditions are met. In-duct experiments showed the ability of a 3D printed SBH silencer to simultaneously enhance acoustic sensing and fully trap the pulse spectral components in the SBH cavities in the presence of a low-speed flow. This study opens up new avenues for the development of dual-purpose silencers designed for acoustic monitoring and noise control in duct systems without obstructing the air flow. Full article
(This article belongs to the Section Acoustics and Vibrations)

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